Axio Scan.Z1

The Axio Scan.Z1 is an automated slide scanner for brightfield, POL and fast fluorescence imaging.

Features

  • Feeder with 100 slide capacity, 4 standard slides per mounting frame
  • 25x mounting frames for 4 slides 76x26 mm provided (100 slides per batch)
  • 1 X Mounting frame for 2 slides 76x52 mm for Axio Scan.Z1
  • Fluorescence illumination via long-lifetime, fast and ultra-stable LEDs (385, 430, 475, 555, 590, 630, 735 nm): Solid-State Light Source Colibri 7, Type FR-R[G/Y]BV-UV
    • Far Red (735nm) for excitation of Cy7 and similar dyes
    • Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes
    • Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes
    • Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes
    • Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar dyes
    • Violet (430nm) for excitation of eCFP, Lucifer Yellow, Alexa 430 and similar dyes 
    • UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes
Axio Scan Z.1 machine at UTS
  • Quad-band filter for DAPI, GFP, Cy3, Cy5 and Cy7 and tri-band filter for CFP and mCherry:
    • Filter Set 112 HE LED (E) for use with the illumination system Colibri 7. Suitable for fluorescent dyes like DAPI, FITC, DsRed, Cy5 and Cy7 with excitation wavelengths 385, 475, 555, 630 and 735 nm. Contains beam splitter PBS 405 + 493 + 575 + 654 + 761 and emission filter PBP 425/30 + 514/31 + 592/25 + 681/45 + 785/38.
    • Filter set 91 HE LED (E) for use with the illumination system Colibri 7. Suitable for fluorescent dyes like CFP, YFP and mCherry with excitation wavelengths 430, 511 and 590 nm. Contains beam splitter TBS 450 + 538 + 610 and emission filter TBP 467/24 + 555/25 + 687/145.
  • Ring Aperture Contrast (RAC) for gentle fluorescence automatic tissue detection and focusing.
  • 2 x High-end cameras for brightfield in colour and high-sensitivity monochrome for fluorescence:
    • Camera Set 3CCD Hitachi HV-F203SCL for Axio Scan.Z1
    • Camera Set Orca Flash 4.0 V3 for Axio Scan.Z1
  • Objectives, high-resolution 5x, 10x, 20x, 20x POL and 40x corr (dry)
    • Objective Fluar 5x/0.25 M27 420130-9900-000
    • Objective Plan-Apochromat 10x/0.45 M27 420640-9900-000
    • Objective Plan-Apochromat 20x/0.8 M27 420650-9902-000
    • Objective N-Achroplan 20x/0.45 Pol M27 420953-9901-000
    • Objective Plan-Apochromat 40x/0.95 Corr M27 420660-9970-000
A complex software interface displays various file management tools and image processing controls.

  • High-end PC workstation with ZEN Blue Software, including integrated image analysis:

    • ZEN 2.6 slidescan basic includes already the functionality of the modules Measurement, Z Stack, Tiles & Positions, Multi Channel, Extended Focus, Autofocus and Experiment Designer.

  • Off-line ZEN software license for off-line image analysis

    • Machine Learning Image Analysis (Pixel Based Segmentation) 

    • ZEN Module Intellesis Hardware License Key Simple and user-friendly tool for image segmentation using pixel classification using supervised machine-learning methods, e.g., the user will provide annotated images to train a model. 

Fluorescent microscopy image showing red and green-stained cells against a black background. The split-screen displays identical high-contrast cellular patterns.