Nikon A1R

The Nikon A1R is an inverted confocal microscope with two standard PMT detectors and two standard GAaSP detectors. A separate 32 channel spectral detector, and transmitted light PMT detector are also available. The system is inside an environmental chamber with temperature, CO2 and humidity control.

Microscope

  • Inverted TiE Stand
  • Motorized x-y stage
  • Piezo z stage
  • Perfect Focusing System

Nikon objectives

The MIF has a range of Nikon objectives that can be installed on Nikon microscopes. Please contact the MIF team if you need a specific objective installed on a microscope prior to your imaging session.

 

Objectives NA Tech WD/mm Imm. PFS Material Ref.
4X Plan Apo 𝝺 0.2 BF 20 Air Y MRD00045
4X Plan Fluor DL F 0.13 PhL 16.4 Air N MRH20041
10X Plan Fluor DLL 0.3 Ph1 16 Air Y MRH10101
10X Plan Apochromat 𝝺 0.45 DIC 4 Air Y MRD00105
20x Plan Apo lambda DM 0.75 Ph2 1 Air Y MRD00205
20X Apo 𝝺S 0.95   0.95 Water Y MRD77200
20X Plan Fluor DM ELWD 0.45 Ph1 8.20 - 6.90 Air Y MRH08230
20X S Plan Fluor LWD 0.7   2.3 - 1.3 Air Y MRH08250
40X Plan Apochromat 𝝺DM  0.95 Ph2 0.21 Air Y MRD30405
40X S Plan Fluor ADM ELWD 0.6 Ph2 3.60 - 2.80 Air Y MRH48230
40X Apo 𝝺S chromatic correction:405-850nm 1.25   0.18 Water Y MRD77400
40X Plan Fluor H N2 1.3 DIC .24 Oil Y MRH01401
60X APlan Apochromat VC 1.2   0.29 Water Y MRD07602
60X Plan Apochromat 𝝺DM 1.4 DIC 0.13 Oil Y MRD31605
100X Plan Apochromat 𝝺  1.45   0.13 Oil Y MRD01905
100X Plan Apo 𝝺DM 1.45 Ph3 0.13 Oil Y MRD31905

Lasers

  • 405, 457, 514, 488, 561, 640 nm
     

Scanners

  • Galvano scanner up to 24 Hz
  • Resonant scanner up to 420 Hz (512 x 512)
     
Nikon A1R image processsed with denoise
Mouse kidney section labelled with DAPI (cyan), AlexaFluor 488 WGA (green) and AlexaFluor 568 Phalloidin (magenta) and acquired with a 20x/0.7 objective.

Software

NIS Elements including FRET module, JOBS for automated acquisition and real time analysis workflows, NIS.ai denoise and deconvolution algorithms for image processing.