The Olympus BX53 upright manual microscope is equipped for brightfield, phase contrast, DIC, polarised light and fluorescence, suitable for imaging slides.
Microscope
- BX53 upright stand
- Manual xyz stage
- Coded nosepiece for electronic communication with software for active objective position
Objectives
| Series Name | Magnification | NA | Working distance (mm) | Cover glass thickness (mm) | Immersion | Imaging Modes |
|---|---|---|---|---|---|---|
| UPLXAPO Plan Apochromat | 4X | 0.16 | 13 | - | Dry | BF, DIC4, POL, FL |
| 10X | 0.4 | 3.1 | 0.17 | Dry | BF, DIC10, POL, FL | |
| 20X | 0.8 | 0.6 | 0.17 | Dry | BF, DIC20, POL, FL | |
| 40X | 0.95 | 0.18 | 0.11 - 0.23 | Dry | BF, DIC40, POL, FL | |
| 40X | 0.75 | 0.18 | 0.17 | Dry | BF, PH2, POL, FL | |
| 100X | 1.45 | 0.13 | 0.17 | Oil | BF, PH3, POL, FL |
Light source
Olympus U-LGPS solid state LED and LDP (laser-diode-pumped phosphor).
Filters
| Filter | Excitation (nm) | Emission (nm) | Dichroic Mirror (nm) |
|---|---|---|---|
| DAPI (U-FBNA) | 360 - 370 | 420 - 460 | 410 |
| FITC (U-FGNA) | 470 - 495 | 510 - 550 | 505 |
| TRITC (U-FUNA) | 540 - 550 | 575 - 625 | 570 |
| Cy5 (U-FF) | 604 - 644 | 672 - 712 | 660 |
Detector
- DP75 12.37-megapixel colour CMOS camera with 22 fps (4096 x 3000) and up to 60 fps (1920 x 1080). Pixel shift acquisition allows 8192 x 6000 resolution.
Software
CellSens Standard software for image acquisition and analysis. Process manager includes Extended Focus Imaging for combined Z-stacks and Multiple Image Alignment for large stitched imaging.