Inverted wide field fluorescence microscope
- Phase contrast and DIC objectives with Perfect Focus System™
- A Nikon Qi2 16.2 megapixel monochrome CCD camera as well and a EMCCD cameras suitable for live-cell imaging.
- Environmental chamber with both temperature and CO_2 control.
- Filter sets include: mCherry (HC Red), GFP, YFP, Texas Red, Cy5.5, DAPI, UV and TRITC.
Objectives
| Objectives | NA | Tech | WD/mm | Imm. | PFS | Material Ref. |
|---|---|---|---|---|---|---|
| 4X Plan Apo 𝝺 | 0.2 | BF | 20 | Air | Y | MRD00045 |
| 10X Plan Fluor DLL | 0.3 | Ph1 | 16 | Air | Y | MRH10101 |
| 20X S Plan Fluor ELWD | 0.45 | Ph1 | 8.20-6.90 | Air | Y | MRH48230 |
| 40X S Plan Fluor ELWD | 0.6 | Ph2 | 3.60-2.80 | Air | Y | MRH48230 |
| 100X Plan Apo VC | 1.4 | DIC | 0.13 | Oil | Y | MRD01901 |
| 100X Plan Apo 𝝺 | 1.45 | Ph3 | 0.13 | Oil | Y | MRD31905 |
Standard Filters Cubes
| Name | Ex | Em | Position |
|---|---|---|---|
| DAPI | 340-380 | 435-485 | 2 |
| CFPHQ | 420-445 | 460-510 | |
| GFP-B | 460-500 | 510-560 | 6 |
| Texas Red | 540-580 | 600-660 | |
| CFL-mCherry | 550-590 | 608-683 | 3 |
| mCherryHQ | 550-590 | 607-682 | |
| Cy5.5 | 635-675 | 695-735 |
Polychroics
BGRFR quad (position 4)
| Name | Ex | Em |
|---|---|---|
| DAPI | 380-395 | 405-470 |
| FITC | 472-500 | 505-530 |
| TRITC | 545-575 | 585-625 |
| Cy5 | 640-650 | 672-730 |
CFP-YFP dual (position 5)
| Name | Ex | Em |
|---|---|---|
| CFP | 400-434 | 450-480 |
| YFP | 490-515 | 525-570 |