Laser scanning confocal inverted microscope.
- Humidified environmental temperature and CO2 control
| Lens | NA | Tech | WD/mm | Imm | pFS |
|---|---|---|---|---|---|
| 20X S Plan Fluor ELWD | 0.45 | Ph1 | Air | ||
| 20X Plan Apo 𝝺 | 0.75 | Ph2 | Air | ||
| 60X Plan Apo 𝝺 | 1.4 | DIC | Oil | ||
| 40X S Plan Fluor ELWD | 0.6 | Ph2 | Air | ||
| 100X Plan Apo | 1.4 | DIC | Oil |
Lasers
| Excitation/mm | Fluorophores |
|---|---|
| 405 | DAPI, Hoechst |
| 488 | TRITC |
| 561 | FITC |
| 637 | Cy5 |
| 458 | CFP |
| 514 | YFP |
NB: the CFP-YFP laser pair are on a separate light path.
Detectors
| Channel | Type | Emission/Nm | |||
|---|---|---|---|---|---|
| 1 | Standard | 425-475 | |||
| 2 | GaAsP | 425-475 | 465-500 | 425-475 | 500-550 |
| 3 | GsAsP | 525-555 | 570-620 | 650- | |
| 4 | Standard | 665-735 |
- Spectral 405-750nm, up to 32 bins with resolutions of 20, 10, 6 or 1 nm
- Transmitted light