Dr Yinghui Chen is a Postdoctoral Research Associate of the ARC research hub for Integrated Devices for End-user Analysis at Low Levels (IDEAL). She is a the core member of the Institute for Biomedical Materials and Devices (IBMD) at the University of Technology Sydney.
Mar.2019: Doctor of Philosophy, University of Technology Sydney
July.2014: Master of Medicine, Liaoning University of Traditional Chinese Medicine
July.2012: Bachelor of Medicine, Liaoning University of Traditional Chinese Medicine
Honours and Awards
2018: Science HDR Student Paper of the Month in December 2017
2015: PhD scholarship of IBMD, UTS
2014: International Macquarie University Research Excellence Scholarship
2013: Second runner-up of thesis in Pharmaceutical Postgraduates’ Academic Forum in North China
2013: Excellent report prize in Pharmaceutical Postgraduates’ Academic Forum in North China
2011: Accessit in the “Merro Cup” Knowledge and Skill of Traditional Chinese Medicine as the representative for Liaoning University of Traditional Chinese Medicine
2011: Forth prize scholarship of Liaoning University of Traditional Chinese Medicine
2011: First prize scholarship of Liaoning University of Traditional Chinese Medicine
2010: Third award in competition of material medica identification of School of Pharmacy, Liaoning University of Traditional Chinese Medicine
2010: Second prize scholarship of Liaoning University of Traditional Chinese Medicine
2010: Forth prize scholarship of Liaoning University of Traditional Chinese Medicine
2009: First prize scholarship in Liaoning University of Traditional Chinese Medicine
2009: Forth prize scholarship in Liaoning University of Traditional Chinese Medicine
Australian Nanotechnolgoy Network
Reviewing services for journals
Analytical Chemistry, Chemistry of Materials, Trends in Analytical Chemistry, Advanced Functional Materials
- Prostate cancer diagnosis
- Nano-biosensor development
- Live cell imaging
- Nanoparticles' surface modification and bioconjugation
© 2020 Elsevier B.V. Lanthanide-activated nanoconstructs (LANCs) have attracted a great attention in optical multiplexing. This review, is the integrated conclusion of reported LANCs being applied as signal codes in optical multiplexing during the past decade. An introduction of the basic concepts and theories about optical multiplexing technique is provided initially. On top of that, the optical advantages of LANCs is comprehensively summarized to illuminate the superiority of LANCs over conventional materials in optical multiplexing. It particularly focuses on the near-infrared (NIR) light excited upconversion (UC) and downshifting (DS) nanomaterials, concerning their diverse and narrow excitation and emission wavelengths, tunable emission lifetime (μs-ms range) and intensity. Subsequently, the exploitation of UC- or DS-emitting LANCs as encoding tools in multiplexing based on various optical parameters is described in detail, including emission wavelength, intensity (ratiometric), temporal lifetime (τ), phase angle and excitation power. Notably, these parameters can not only work by themselves to be encoders, but also ally with each other to remarkably enrich the encoding capability of LANCs. In addition, the attractive dual-modal DS and UC emission-based multiplexing is emphasized. Finally, a summary of the challenges faced by LANCs-based optical multiplexing and a discussion of their future development is given.
Chen, Y, D'Amario, C, Gee, A, Duong, HTT, Shimoni, O & Valenzuela, SM 2020, 'Dispersion stability and biocompatibility of four ligand-exchanged NaYF4: Yb, Er upconversion nanoparticles.', Acta Biomaterialia, vol. 102, pp. 384-393.View/Download from: Publisher's site
Surface modification to obtain high dispersion stability and biocompatibility is a key factor for bio-application of upconversion nanoparticles (UCNPs). A systematic study of UCNPs modified with four hydrophilic molecules separately, comparing their dispersion stability in biological buffers and cellular biocompatibility is reported here. The results show that carboxyl-functionalized UCNPs (modified by 3,4-dihydrocinnamic acid (DHCA) or poly(monoacryloxyethyl phosphate (MAEP)) with negative surface charge have superior even-distribution in biological buffers compared to amino-functionalized UCNPs (modified by (aminomethyl)phosphonic (AMPA) or (3-Aminopropyl)triethoxysilane (APTES)) with positive surface charge. Subsequent investigation of cellular interactions revealed high levels of non-targeted cellular uptake of the particles modified with either of the three small molecules (AMPA, APTES, DHCA) and high levels of cytotoxicity when used at high concentrations. The particles were seen to be trapped as particle-aggregates within the cellular cytoplasm, leading to reduced cell viability and cell proliferation, along with dysregulation of the cell cycle as assessed by DNA content measurements. The dramatically reduced proportion of cells in G1 phase and the slightly increased proportion in G2 phase indicates inhibition of M phase, and the appearance of sub-G1 phase reflects cell necrosis. In contrast, MAEP-modified UCNPs are bio-friendly with increased dispersion stability in biological buffers, are non-cytotoxic, with negligible levels of non-specific cellular uptake and no effect on the cell cycle at both low and high concentrations. MAEP-modified UCNPs were further functionalized with streptavidin for intracellular microtubule imaging, and showed clear cytoskeletal structures via their upconversion luminescence. STATEMENT OF SIGNIFICANCE: Upconversion nanoparticles (UCNP) are an exciting potential nanomaterial for bio-applications. Their anti-Stokes luminescence...
Zhou, Y, Chen, Y, He, H, Liao, J, Duong, HTT, Parviz, M & Jin, D 2019, 'A homogeneous DNA assay by recovering inhibited emission of rare earth ions-doped upconversion nanoparticles', Journal of Rare Earths, vol. 37, no. 1, pp. 11-18.View/Download from: Publisher's site
© 2018 Chinese Society of Rare Earths Robust and easy-to-use kits specific for a particular DNA sequence are desirable for early detection of diseases. However, the major challenge with these tests is often the background fluorescence artifacts arising from biological species due to employing UV and visible range of light. Here, we have reported a near-infrared (NIR) fluorescence “turn-on” kit based on rare earth ions doped nanoparticles, upconversion nanoparticles (UCNPs), and gold nanoparticles (AuNPs), which forms a fluorescence-quencher pair, brought together by a hairpin structure through the formation of double-stranded DNA (dsDNA), with quenched upconversion luminescence. In the presence of analytes, the molecular beacon opens to push AuNPs away from UCNPs, with a distance longer than the efficient quenching distance, so that the inhibited upconversion emission will be restored. We demonstrated that this assay provides a homogeneous, facile, simple and highly selective HIV-1 based DNA detection system with restore efficiency up to 85%, and the detection limit of 5 nm.
Chen, Y, Duong, HTT, Wen, S, Mi, C, Zhou, Y, Shimoni, O, Valenzuela, SM & Jin, D 2018, 'Exonuclease III-Assisted Upconversion Resonance Energy Transfer in a Wash-Free Suspension DNA Assay.', Analytical Chemistry, vol. 90, no. 1, pp. 663-668.View/Download from: Publisher's site
Sensitivity is the key in optical detection of low-abundant analytes, such as circulating RNA or DNA. The enzyme Exonuclease III (Exo III) is a useful tool in this regard; its ability to recycle target DNA molecules results in markedly improved detection sensitivity. Lower limits of detection may be further achieved if the detection background of autofluorescence can be removed. Here we report an ultrasensitive and specific method to quantify trace amounts of DNA analytes in a wash-free suspension assay. In the presence of target DNA, the Exo III recycles the target DNA by selectively digesting the dye-tagged sequence-matched probe DNA strand only, so that the amount of free dye removed from the probe DNA is proportional to the number of target DNAs. Remaining intact probe DNAs are then bound onto upconversion nanoparticles (energy donor), which allows for upconversion luminescence resonance energy transfer (LRET) that can be used to quantify the difference between the free dye and tagged dye (energy acceptor). This scheme simply avoids both autofluorescence under infrared excitation and many tedious washing steps, as the free dye molecules are physically located away from the nanoparticle surface, and as such they remain "dark" in suspension. Compared to alternative approaches requiring enzyme-assisted amplification on the nanoparticle surface, introduction of probe DNAs onto nanoparticles only after DNA hybridization and signal amplification steps effectively avoids steric hindrance. Via this approach, we have achieved a detection limit of 15 pM in LRET assays of human immunodeficiency viral DNA.
Duong, HTT, Chen, Y, Tawfik, SA, Wen, S, Parviz, M, Shimoni, O & Ab, DJ 2018, 'Systematic investigation of functional ligands for colloidal stable upconversion nanoparticles†', RSC Advances, vol. 8, no. 9, pp. 4842-4849.View/Download from: Publisher's site
© The Royal Society of Chemistry 2018. Despite intense efforts on surface functionalization to generate hydrophilic upconversion nanoparticles (UCNPs), long-term colloidal stability in physiological buffers remains a major concern. Here we quantitatively investigate the competitive adsorption of phosphate, carboxylic acid and sulphonic acid onto the surface of UCNPs and study their binding strength to identify the best conjugation strategy. To achieve this, we designed and synthesized three di-block copolymers composed of poly(ethylene glycol) methyl ether acrylate and a polymer block bearing phosphate, carboxylic or sulphonic acid anchoring groups prepared by an advanced polymerization technique, Reversible Addition Fragmentation Chain Transfer (RAFT). Analytical tools provide the evidence that phosphate ligands completely replaced all the oleic acid capping molecules on the surface of the UCNPs compared with incomplete ligand exchange by carboxylic and sulphonic acid groups. Meanwhile, simulated quantitative adsorption energy measurements confirmed that among the three functional groups, the calculated adsorption strength for phosphate anchoring ligands is higher which is in good agreement with experimental results regarding the best colloidal stability, especially in phosphate buffer solution. This finding suggests that polymers with multiple anchoring negatively charged phosphate moieties provide excellent colloidal stability for lanthanide ion-doped luminescent nanoparticles for various potential applications.
He, H, Howard, CB, Chen, Y, Wen, S, Lin, G, Zhou, J, Thurecht, KJ & Jin, D 2018, 'Bispecific Antibody-Functionalized Upconversion Nanoprobe.', Analytical chemistry, vol. 90, no. 5, pp. 3024-3029.View/Download from: Publisher's site
Upconversion nanoparticles (UCNPs) are new optical probes for biological applications. For specific biomolecular recognition to be realized for diagnosis and imaging, the key lies in developing a stable and easy-to-use bioconjugation method for antibody modification. Current methods are not yet satisfactory regarding conjugation time, stability, and binding efficiency. Here, we report a facile and high-yield approach based on a bispecific antibody (BsAb) free of chemical reaction steps. One end of the BsAb is designed to recognize methoxy polyethylene glycol-coated UCNPs, and the other end of the BsAb is designed to recognize the cancer antigen biomarker. Through simple vortexing, BsAb-UCNP nanoprobes form within 30 min and show higher (up to 54%) association to the target than that of the traditional UCNP nanoprobes in the ELISA-like assay. We further demonstrate its successful binding to the cancer cells with high efficiency and specificity for background-free fluorescence imaging under near-infrared excitation. This method suggests a general approach broadly suitable for functionalizing a range of nanoparticles to specifically target biomolecules.
Aixia, J & Qiuhong, L 2015, 'Pharmacokinetics, tissue distribution and excretion study of tetrandrine in rats', Journal of Chinese Pharmaceutical Sciences, vol. 24, no. 8.View/Download from: Publisher's site
Lu, J, Chen, Y, Liu, D, Ren, W, Lu, Y, Shi, Y, Piper, JA, Paulsen, IT & Jin, D 2015, 'One-step Protein Conjugation to Upconversion Nanoparticles', Analytical Chemistry, vol. 87, no. 20, pp. 10406-10413.View/Download from: Publisher's site
The emerging upconversion nanoparticles offer a fascinating library of ultrasensitive luminescent probes for a range of biotechnology applications from biomarker discovery to single molecule tracking, early disease diagnosis, deep tissue imaging, and drug delivery and therapies. The effective bioconjugation of inorganic nanoparticles to the molecule-specific proteins, free of agglomeration, nonspecific binding, or biomolecule deactivation, is crucial for molecular recognition of target molecules or cells. The current available protocols require multiple steps which can lead to low probe stability, specificity, and reproducibility. Here we report a simple and rapid protein bioconjugation method based on a one-step ligand exchange using the DNAs as the linker. Our method benefits from the robust DNA–protein conjugates as well as from multiple ions binding capability. Protein can be preconjugated via an amino group at the 3′ end of a synthetic DNA molecule, so that the 5′ end phosphoric acid group and multiple phosphate oxygen atoms in the phosphodiester bonds are exposed to replace the oleic acid ligands on the surface of upconversion nanoparticles due to their stronger chelating capability to lanthanides. We demonstrated that our method can efficiently pull out the upconversion nanoparticles from organic solvent into an aqueous phase. The upconversion nanoparticles then become hydrophilic, stable, and specific biomolecules recognition. This allows us to successfully functionalize the upconversion nanoparticles with horseradish peroxidise (HRP) for catalytic colorimetric assay and for streptavidin (SA)–biotin immunoassays.
Chen, Y, Xu, Q, Zhang, W, Li, R, Wang, Y, Xue, H, Yin, J, Lu, D, Ying, X & Kang, T 2014, 'HPLC DETERMINATION OF VITEXIN-4 ''-O-GLUCOSIDE IN MOUSE PLASMA AND TISSUES AFTER ORAL AND INTRAVENOUS ADMINISTRATION', JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, vol. 37, no. 7, pp. 1052-1064.View/Download from: Publisher's site
Wei, W, Ying, X, Zhang, W, Chen, Y, Leng, A, Jiang, C & Liu, J 2014, 'Effects of vitexin-2 ''-O-rhamnoside and vitexin-4 ''-O-glucoside on growth and oxidative stress-induced cell apoptosis of human adipose-derived stem cells', JOURNAL OF PHARMACY AND PHARMACOLOGY, vol. 66, no. 7, pp. 988-997.View/Download from: Publisher's site
Cai, S, Chen, Y, Zhang, W & Ying, X 2013, 'Comparative study on the excretion of vitexin-4 ''-O-glucoside in mice after oral and intravenous administration by using HPLC', BIOMEDICAL CHROMATOGRAPHY, vol. 27, no. 11, pp. 1375-1379.View/Download from: Publisher's site
Chen, Y, Zhang, W, Li, D, Ai, J, Meng, Y, Ying, X & Kang, T 2013, 'Hepatic and gastrointestinal first-pass effects of vitexin-4-O-glucoside in rats', JOURNAL OF PHARMACY AND PHARMACOLOGY, vol. 65, no. 10, pp. 1500-1507.View/Download from: Publisher's site
Xue, H, Li, Y, Zhang, W, Lu, D, Chen, Y, Yin, J, Meng, Y, Ying, X & Kang, T 2013, 'Pharmacokinetic study of isoquercitrin in rat plasma after intravenous administration at three different doses', BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 49, no. 3, pp. 435-441.View/Download from: Publisher's site