Sun, Y, Zhang, W, Wang, B, Xu, X, Chou, J, Shimoni, O, Ung, AT & Jin, D 2018, 'A supramolecular self-assembly strategy for upconversion nanoparticle bioconjugation.', Chemical communications (Cambridge, England), vol. 54, no. 31, pp. 3851-3854.View/Download from: UTS OPUS or Publisher's site
An efficient surface modification for upconversion nanoparticles (UCNPs) is reported via supramolecular host-guest self-assembly. Cucurbituril (CB) can provide a hydrophilic surface and cavities for most biomolecules. High biological efficiency, activity and versatility of the approach enable UCNPs to be significantly applied in bio-imaging, early disease detection, and bio-sensing.
Jin, D, Xi, P, Wang, B, Zhang, L, Enderlein, J & van Oijen, AM 2018, 'Nanoparticles for super-resolution microscopy and single-molecule tracking.', Nature methods, vol. 15, no. 6, pp. 415-423.View/Download from: UTS OPUS or Publisher's site
We review the use of luminescent nanoparticles in super-resolution imaging and single-molecule tracking, and showcase novel approaches to super-resolution imaging that leverage the brightness, stability, and unique optical-switching properties of these nanoparticles. We also discuss the challenges associated with their use in biological systems, including intracellular delivery and molecular targeting. In doing so, we hope to provide practical guidance for biologists and continue to bridge the fields of super-resolution imaging and nanoparticle engineering to support their mutual advancement.
Wang, DJ, Di, XJ, Wang, BM, Wang, F, Guo, ZY & Jin, DY 2018, 'Advances in single particle tracking in living cells', Chinese Optics, vol. 11, no. 3, pp. 281-295.View/Download from: UTS OPUS or Publisher's site
© 2018, China Science Publishing & Media LTD. All right reserved. Single particle tracking(SPT) technique locates and tracks individual fluorescent or scattering particles within a cell with the help of microscope system. Based on the ability of real-time monitoring of the complex and highly dynamic changes in tissue structure within living cells and the ability to provide dynamic relationships between structure and function, SPT has important applications in cell biology. In this review,the mechanism of SPT and its application on cells are summarized. Firstly, the dynamics of SPT are introduced, including single particle localization, trajectory reconstruction and analysis. Then the optical materials and instruments that SPT technology focuses on at the present stage are described. Finally, the application of SPT in cell membrane, intracellular signaling pathway, molecular transport mechanism, genetic information expression, and viral infection mechanism are proposed. In addition, the advance of SPT technology are prospected in this paper.
Chen, C, Wang, F, Wen, S, Su, QP, Wu, MCL, Liu, Y, Wang, B, Li, D, Shan, X, Kianinia, M, Aharonovich, I, Toth, M, Jackson, SP, Xi, P & Jin, D 2018, 'Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles.', Nature communications, vol. 9, no. 1.View/Download from: UTS OPUS or Publisher's site
Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spatial alignment and temporal synchronization of high power (femtosecond) lasers, which is limited by the inefficiency of the probes. Here, we report that upconversion nanoparticles (UCNPs) can unlock a new mode of near-infrared emission saturation (NIRES) nanoscopy for deep tissue super-resolution imaging with excitation intensity several orders of magnitude lower than that required by conventional MPM dyes. Using a doughnut beam excitation from a 980 nm diode laser and detecting at 800 nm, we achieve a resolution of sub 50 nm, 1/20th of the excitation wavelength, in imaging of single UCNP through 93 μm thick liver tissue. This method offers a simple solution for deep tissue super resolution imaging and single molecule tracking.
Wang, F, Wen, S, He, H, Wang, B, Zhou, Z, Shimoni, O & Jin, D 2018, 'Microscopic inspection and tracking of single upconversion nanoparticles in living cells', Light: Science and Applications, vol. 7, no. 4.View/Download from: UTS OPUS or Publisher's site
© 2018 The Author(s). Nanoparticles have become new tools for cell biology imaging, sub-cellular sensing, super-resolution imaging, and drug delivery. Long-term 3D tracking of nanoparticles and their intracellular motions have advanced the understanding of endocytosis and exocytosis as well as of active transport processes. The sophisticated operation of correlative optical-electron microscopy and scientific-grade cameras is often used to study intercellular processes. Nonetheless, most of these studies are still limited by the insufficient sensitivity for separating a single nanoparticle from a cluster of nanoparticles or their aggregates8. Here we report that our eyes can track a single fluorescent nanoparticle that emits over 4000 photons per 100 milliseconds under a simple microscope setup. By tracking a single nanoparticle with high temporal, spectral and spatial resolution, we show the measurement of the local viscosity of the intracellular environment. Moreover, beyond the colour domain and 3D position, we introduce excitation power density as the fifth dimension for our eyes to simultaneously discriminate multiple sets of single nanoparticles.