Tanaka, A, Allam, VSRR, Simpson, J, Tiberti, N, Shiels, J, To, J, Lund, M, Combes, V, Weldon, S, Taggart, C, Dalton, JP, Phipps, S, Sukkar, MB & Donnelly, S 2018, 'The parasitic 68-mer peptide FhHDM-1 inhibits mixed granulocytic inflammation and airway hyperreactivity in experimental asthma', JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 141, no. 6, pp. 2316-2319.View/Download from: UTS OPUS or Publisher's site
Wong, SL, To, J, Santos, J, Allam, VSRR, Dalton, JP, Djordjevic, SP, Donnelly, S, Padula, MP & Sukkar, MB 2018, 'Proteomic Analysis of Extracellular HMGB1 Identifies Binding Partners and Exposes Its Potential Role in Airway Epithelial Cell Homeostasis.', Journal of Proteome Research, vol. 17, no. 1, pp. 33-45.View/Download from: UTS OPUS or Publisher's site
The release of damage-associated molecular patterns (DAMPs) by airway epithelial cells is believed to play a crucial role in the initiation and development of chronic airway conditions such as asthma and chronic obstructive pulmonary disease (COPD). Intriguingly, the classic DAMP high-mobility group box-1 (HMGB1) is detected in the culture supernatant of airway epithelial cells under basal conditions, indicating a role for HMGB1 in the regulation of epithelial cellular and immune homeostasis. To gain contextual insight into the potential role of HMGB1 in airway epithelial cell homeostasis, we used the orthogonal and complementary methods of high-resolution clear native electrophoresis, immunoprecipitation, and pull-downs coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) to profile HMGB1 and its binding partners in the culture supernatant of unstimulated airway epithelial cells. We found that HMGB1 presents exclusively as a protein complex under basal conditions. Moreover, protein network analysis performed on 185 binding proteins revealed 14 that directly associate with HMGB1: amyloid precursor protein, F-actin-capping protein subunit alpha-1 (CAPZA1), glyceraldehyde-3 phosphate dehydrogenase (GAPDH), ubiquitin, several members of the heat shock protein family (HSPA8, HSP90B1, HSP90AA1), XRCC5 and XRCC6, high mobility group A1 (HMGA1), histone 3 (H3F3B), the FACT (facilitates chromatin transcription) complex constituents SUPT1H and SSRP1, and heterogeneous ribonucleoprotein K (HNRNPK). These studies provide a new understanding of the extracellular functions of HMGB1 in cellular and immune homeostasis at the airway mucosal surface and could have implications for therapeutic targeting.
Annapurna, VV, Hemalatha, R, Raviteja, A, Ramaraju, AVS, Narendra Babu, K, Thirupathaiah, Y, Mohd, S, Harishankar, N & Balakrishna, N 2015, 'Selective cecal bacterial changes mediate the adverse effects associated with high palmolein or high starch diets: Prophylactic role of flax oil', International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, pp. 89-95.
© 2015, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved. Objective: Studies on the dynamics of gut bacteria in relation to metabolic adverse effects induced by high palmolein or high starch diets and in relation to health benefits of uncommon foods are lacking. Our aim was to assess under controlled conditions, the impact of vegetable based palmitic acid rich, high fat diet or a high starch diet on various metabolic parameters in relation to selective gut bacterial alterations in rats and also to see the effect of flaxseed oil supplementation on these parameters. Methods: Wistar Rats were fed for 4 mo either a control diet(CT) or a 30% high fat diet (HF) or HF diet with flax oil supplemented at two different doses (HFF1 and HFF2) or a 78% high starch diet (HC) after which they were sacrificed and analyzed for selective cecal bacteria, hematology, immune function and body composition. Results: High palmolein diet fed rats showed a decrease in colony forming units of lactobacillus, enterococci, streptococci bacteria and an increase in enterobacteriaceae in the cecum unlike HC fed rats. While high palmolein diet was found to impair immunity and increase inflammation, high starch diet affected body composition and lipid profile. Supplementing the flax seed oil ameliorated most of the adverse effects of high palmolein diet. Conclusions: Independent of energy intakes both high palmolein and high starch intakes have differential adverse effects. It can be envisaged that the adverse effects of feeding palmolein are mediated through immune impairment and inflammatory response, which in turn are associated with altered gut bacteria profile; and flax oil was found to have a prophylactic role in controlling these adverse effects. This study emphasizes the need to evaluate immunological as well as bacterial profile while assessing the safety of dietary fats in addition to traditional methods.
Jonnalagadda, VG, Ram Raju, AVS, Pittala, S, Shaik, A & Selkar, NA 2014, 'The prelude on novel receptor and ligand targets involved in the treatment of diabetes mellitus.', Advanced pharmaceutical bulletin, vol. 4, no. 3, pp. 209-217.View/Download from: Publisher's site
Metabolic disorders are a group of disorders, due to the disruption of the normal metabolic process at a cellular level. Diabetes Mellitus and Tyrosinaemia are the majorly reported metabolic disorders. Among them, Diabetes Mellitus is a one of the leading metabolic syndrome, affecting 5 to 7 % of the population worldwide and mainly characterised by elevated levels of glucose and is associated with two types of physiological event disturbances such as impaired insulin secretion and insulin resistance. Up to now, various treatment strategies are like insulin, alphaglucosidase inhibitors, biguanides, incretins were being followed. Concurrently, various novel therapeutic strategies are required to advance the therapy of Diabetes mellitus. For the last few decades, there has been an extensive research in understanding the metabolic pathways involved in Diabetes Mellitus at the cellular level and having the profound knowledge on cell-growth, cell-cycle, and apoptosis at a molecular level provides new targets for the treatment of Diabetes Mellitus. Receptor signalling has been involved in these mechanisms, to translate the information coming from outside. To understand the various receptors involved in these pathways, we must have a sound knowledge on receptors and ligands involved in it. This review mainly summarises the receptors and ligands which are involved the Diabetes Mellitus. Finally, researchers have to develop the alternative chemical moieties that retain their affinity to receptors and efficacy. Diabetes Mellitus being a metabolic disorder due to the glucose surfeit, demands the need for regular exercise along with dietary changes.
Allam, V, Lam, M, Sukkar, M & Bourke, JE 2017, 'Role Of Pattern Recognition Receptors In The Regulation Of Intrapulmonary Airway Contraction Following Short-Term Cigarette Smoke Exposure In Mice', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, International Conference of the American-Thoracic-Society (ATS), AMER THORACIC SOC, Washington, DC.
Allam, VSRR, Tanaka, A, Dalton, J, Sukkar, M & Donnelly, S 2017, 'A NOVEL PEPTIDE FROM THE LIVER FLUKE FASCIOLA HEPATICA INHIBITS THE MIXED GRANULOCYTIC AIRWAY INFLAMMATION IN A MOUSE MODEL OF ALLERGIC ASTHMA', RESPIROLOGY, WILEY, pp. 81-81.