Ahadi, A, Brennan, S, Kennedy, P, Hutvagner, G & Tran, N 2016, 'Long non-coding RNAs harboring miRNA seed regions are enriched in prostate cancer exosomes', Scientific Reports, vol. 6, pp. 1-14.View/Download from: Publisher's site
Long non-coding RNAs (lncRNAs) form the largest transcript class in the human transcriptome. These
lncRNA are expressed not only in the cells, but they are also present in the cell-derived extracellular
vesicles such as exosomes. The function of these lncRNAs in cancer biology is not entirely clear, but they
appear to be modulators of gene expression. In this study, we characterize the expression of lncRNAs
in several prostate cancer exosomes and their parental cell lines. We show that certain lncRNAs are
enriched in cancer exosomes with the overall expression signatures varying across cell lines. These
exosomal lncRNAs are themselves enriched for miRNA seeds with a preference for let-7 family members
as well as miR-17, miR-18a, miR-20a, miR-93 and miR-106b. The enrichment of miRNA seed regions in
exosomal lncRNAs is matched with a concomitant high expression of the same miRNA. In addition, the
exosomal lncRNAs also showed an over representation of RNA binding protein binding motifs. The two
most common motifs belonged to ELAVL1 and RBMX. Given the enrichment of miRNA and RBP sites on
exosomal lncRNAs, their interplay may suggest a possible function in prostate cancer carcinogenesis
Habib, R, Brennan, S, Tan, M, Larsen, SR & Martiniello-Wilks, R 2015, 'UNDERSTANDING AND HARNESSING MESENCHYMAL STEM CELL HOMING TO METASTATIC PROSTATE CANCER', JOURNAL OF GENE MEDICINE, 9th Biennial Meeting of the Australasian-Gene-and-Cell-Therapy-Society (AGCTS), WILEY-BLACKWELL, Univ Melbourne, Univ Coll, Parkville, AUSTRALIA, pp. 200-201.
Brennan, S, Tran, N, Clayton, A, Webber, J, Cozzi, P & Martiniello-Wilks, R 2015, 'EXOSOMAL MICRORNA: POTENTIAL URINARY AND PLASMA BASED BIOMARKERS FOR THE DIAGNOSIS AND PROGNOSIS OF PROSTATE CANCER', JOURNAL OF GENE MEDICINE, 9th Biennial Meeting of the Australasian-Gene-and-Cell-Therapy-Society (AGCTS), WILEY-BLACKWELL, Univ Melbourne, Univ Coll, Parkville, AUSTRALIA, pp. 203-204.