I am an independent Principal Investigator and Lecturer at the University of Technology Sydney. My expertise and passion is in the areas of cell biophysics and cell engineering. I envisage a future where we understand and treat the physics of disease at the cellular level. I have been a postdoc at MIT (USA) and ETH Zurich (Switzerland), and have completed a PhD at the Max Planck Institute and TU Dresden (Germany). I have gained rich experience from an incredible set of mentors: Tony Hyman, Daniel Muller, Bob Langer, Klavs Jensen and their networks. My goal is to establish systems that address key global challenges in biomedical science and technology to help those suffering from chronic disease around the world.
AWARDS, PRIZES, & FELLOWSHIPS:
- 2016: Life Sciences Research Foundation (LSRF) Postdoctoral Fellowship (sponsored by Good Ventures)
- 2016: Sir Keith Murdoch Fellowship, American Australian Association
- 2016: Broadnext10 catalytic steps grant recipient, Broad Institute
- 2014: HFSP long-term postdoctoral fellowship (declined)
- 2014: Swiss NSF advanced postdoc mobility fellowship
- 2014: RIKEN foreign post doctoral researcher fellowship (declined)
- 2012: PhD grade: Summa Cum Laude, TU Dresden, Germany (high grade possible)
Can supervise: YES
- Intracellular Delivery
Low-Cost Low-Toxicity Cancer Treatments
Medical Devices and Diagnostics
Stewart, MP, Helenius, J, Toyoda, Y, Ramanathan, SP, Muller, DJ & Hyman, AA 2019, 'Publisher Correction: Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding.', Nature, vol. 571, no. 7764, pp. E5-E5.View/Download from: Publisher's site
Change history: In Fig. 1b and c of this Letter, the inset times in the DIC and GFP microscopy images should be in minutes ('min') instead of seconds ('s'). This has not been corrected online.
Stewart, MP, Langer, R & Jensen, KF 2018, 'Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts', CHEMICAL REVIEWS, vol. 118, no. 16, pp. 7409-7531.View/Download from: Publisher's site
Ding, X, Stewart, MP, Sharei, A, Weaver, JC, Langer, RS & Jensen, KF 2017, 'High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption', NATURE BIOMEDICAL ENGINEERING, vol. 1, no. 3.View/Download from: Publisher's site
Toyoda, Y, Cattin, CJ, Stewart, MP, Poser, I, Theis, M, Kurzchalia, TV, Buchholz, F, Hyman, AA & Müller, DJ 2017, 'Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding.', Nature Communications, vol. 8, no. 1, pp. 1-11.View/Download from: Publisher's site
To divide, most animal cells drastically change shape and round up against extracellular confinement. Mitotic cells facilitate this process by generating intracellular pressure, which the contractile actomyosin cortex directs into shape. Here, we introduce a genome-scale microcantilever- and RNAi-based approach to phenotype the contribution of > 1000 genes to the rounding of single mitotic cells against confinement. Our screen analyzes the rounding force, pressure and volume of mitotic cells and localizes selected proteins. We identify 49 genes relevant for mitotic rounding, a large portion of which have not previously been linked to mitosis or cell mechanics. Among these, depleting the endoplasmic reticulum-localized protein FAM134A impairs mitotic progression by affecting metaphase plate alignment and pressure generation by delocalizing cortical myosin II. Furthermore, silencing the DJ-1 gene uncovers a link between mitochondria-associated Parkinson's disease and mitotic pressure. We conclude that mechanical phenotyping is a powerful approach to study the mechanisms governing cell shape.
Stewart, MP, Lorenz, A, Dahlman, J & Sahay, G 2016, 'Challenges in carrier-mediated intracellular delivery: moving beyond endosomal barriers', WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY, vol. 8, no. 3, pp. 465-478.View/Download from: Publisher's site
Stewart, MP, Sharei, A, Ding, X, Sahay, G, Langer, R & Jensen, KF 2016, 'In vitro and ex vivo strategies for intracellular delivery', NATURE, vol. 538, no. 7624, pp. 183-192.View/Download from: Publisher's site
Cattin, CJ, Dueggelin, M, Martinez-Martin, D, Gerber, C, Mueller, DJ & Stewart, MP 2015, 'Mechanical control of mitotic progression in single animal cells', PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 112, no. 36, pp. 11258-11263.View/Download from: Publisher's site
Ramanathan, SP, Helenius, J, Stewart, MP, Catlin, CJ, Hyman, AA & Muller, DJ 2015, 'Cdk1-dependent mitotic enrichment of cortical myosin II promotes cell rounding against confinement', NATURE CELL BIOLOGY, vol. 17, no. 2, pp. 148-+.View/Download from: Publisher's site
Sorce, B, Escobedo, C, Toyoda, Y, Stewart, MP, Cattin, CJ, Newton, R, Banerjee, I, Stettler, A, Roska, B, Eaton, S, Hyman, AA, Hierlemann, A & Mueller, DJ 2015, 'Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement', NATURE COMMUNICATIONS, vol. 6.View/Download from: Publisher's site
Toyoda, Y, Erkut, C, Pan-Montojo, F, Boland, S, Stewart, MP, Mueller, DJ, Wurst, W, Hyman, AA & Kurzchalia, TV 2014, 'Products of the Parkinson's disease-related glyoxalase DJ-1, D-lactate and glycolate, support mitochondrial membrane potential and neuronal survival', BIOLOGY OPEN, vol. 3, no. 8, pp. 777-784.View/Download from: Publisher's site
Stewart, MP, Hodel, AW, Spielhofer, A, Cattin, CJ, Mueller, DJ & Helenius, J 2013, 'Wedged AFM-cantilevers for parallel plate cell mechanics', METHODS, vol. 60, no. 2, pp. 186-194.View/Download from: Publisher's site
Stewart, MP, Toyoda, Y, Hyman, AA & Mueller, DJ 2012, 'Tracking mechanics and volume of globular cells with atomic force microscopy using a constant-height clamp', NATURE PROTOCOLS, vol. 7, no. 1, pp. 143-154.View/Download from: Publisher's site
Stewart, MP, Helenius, J, Toyoda, Y, Ramanathan, SP, Muller, DJ & Hyman, AA 2011, 'Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding', NATURE, vol. 469, no. 7329, pp. 226-230.View/Download from: Publisher's site
Toyoda, Y, Stewart, MP, Hyman, AA & Müller, DJ 2011, 'Atomic force microscopy to study mechanics of living mitotic mammalian cells', Japanese Journal of Applied Physics.View/Download from: Publisher's site
While biochemical pathways within mitotic cells have been intensively studied, the mechanics of dividing cells is only poorly understood. In our recent report, an experimental system combining fluorescence and atomic force microscopy was set up to study dynamics of mitotic rounding of mammalian cells. We show that cells have a rounding pressure that increases upon mitotic entry. Using specific inhibitors or perturbations, we revealed biological processes required for force generation that underpin the cell rounding shape change during mitosis. The significance of the finding and an outlook are discussed. © 2011 The Japan Society of Applied Physics.
- Massachusetts Institute of Technology
- ETH Zurich
- IIT India