Fouani, L, Huang, MLH, Cole, L, Jansson, PJ, Kovacevic, Z & Richardson, DR 2020, 'During mitosis ZEB1 "switches" from being a chromatin-bound epithelial gene repressor, to become a microtubule-associated protein', Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1867, no. 7.View/Download from: Publisher's site
© 2020 Elsevier B.V. Microtubules are polymers of α/β-tubulin, with microtubule organization being regulated by microtubule-associated proteins (MAPs). Herein, we describe a novel role for the epithelial gene repressor, zinc finger E-box-binding homeobox 1 (ZEB1), that "switches" from a chromatin-associated protein during interphase, to a MAP that associates with α-, β- and γ-tubulin during mitosis. Additionally, ZEB1 was also demonstrated to associate with γ-tubulin at the microtubule organizing center (MTOC). Using confocal microscopy, ZEB1 localization was predominantly nuclear during interphase, with α/β-tubulin being primarily cytoplasmic and the association between these proteins being minimal. However, during the stages of mitosis, ZEB1 co-localization with α-, β-, and γ-tubulin was significantly increased, with the association commonly peaking during metaphase in multiple tumor cell-types. ZEB1 was also observed to accumulate in the cleavage furrow during cytokinesis. The increased interaction between ZEB1 and α-tubulin during mitosis was also confirmed using the proximity ligation assay. In contrast to ZEB1, its paralog ZEB2, was mainly perinuclear and cytoplasmic during interphase, showing some co-localization with α-tubulin during mitosis. Considering the association between ZEB1 with α/β/γ-tubulin during mitosis, studies investigated ZEB1's role in the cell cycle. Silencing ZEB1 resulted in a G2-M arrest, which could be mediated by the up-regulation of p21Waf1/Cip1 and p27Kip1 that are known downstream targets repressed by ZEB1. However, it cannot be excluded the G2/M arrest observed after ZEB1 silencing is not due to its roles as a MAP. Collectively, ZEB1 plays a role as a MAP during mitosis and could be functionally involved in this process.
Kalam, SN, Cole, L, Lindsay, L & Murphy, CR 2020, 'Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells.', Reproduction (Cambridge, England), vol. 160, no. 4, pp. 533-546.View/Download from: Publisher's site
Luminal uterine epithelial cells (UEC) have a surge in vesicular activity during early uterine receptivity. It has been predicted these vesicles exit the UEC via exocytosis resulting in secretion and membrane trafficking. The present study investigated the changes in SNARE proteins VAMP2 (v-SNARE) and syntaxin 3 (t-SNARE) localisation and abundance in UECs during early pregnancy in the rat. We found VAMP2 and syntaxin 3 are significantly higher on day 5.5 compared to day 1 of pregnancy. On day 5.5, VAMP2 is perinuclear and syntaxin 3 is concentrated in the apical cytoplasm compared to a cytoplasmic localisation on day 1. This change in localisation and abundance show VAMP2 and syntaxin 3 are involved in vesicular movement and membrane trafficking in UECs during early pregnancy. This study also investigated the influence of cytoskeletal disruption of microtubules and actin filaments on VAMP2 and syntaxin 3 in UECs grown in vitro, since microtubules and actin influence vesicle trafficking. As expected, this study found disruption to microtubules with colchicine and actin with cytochalasin D impacted VAMP2 and syntaxin 3 localisation. These results suggest VAMP2 and syntaxin 3 are involved in the timely trafficking of vesicular membranes to the apical surface in UECs during early pregnancy, as are of microtubules and actin.
Abboud, M, Rybchyn, MS, Liu, J, Ning, Y, Gordon-Thomson, C, Brennan-Speranza, TC, Cole, L, Greenfield, H, Fraser, DR & Mason, RS 2017, 'The effect of parathyroid hormone on the uptake and retention of 25-hydroxyvitamin D in skeletal muscle cells', JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 173, pp. 173-179.View/Download from: Publisher's site
Hu, P, Thinschmidt, JS, Caballero, S, Adamson, S, Cole, L, Chan-Ling, T & Grant, MB 2015, 'Loss of survival factors and activation of inflammatory cascades in brain sympathetic centers in type 1 diabetic mice', AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, vol. 308, no. 8, pp. E688-E698.View/Download from: Publisher's site
Abboud, M, Gordon-Thomson, C, Hoy, AJ, Balaban, S, Rybchyn, MS, Cole, L, Su, Y, Brennan-Speranza, TC, Fraser, DR & Mason, RS 2014, 'Uptake of 25-hydroxyvitamin D by muscle and fat cells', JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 144, pp. 232-236.View/Download from: Publisher's site
Tse, N, Morsch, M, Ghazanfari, N, Cole, L, Visvanathan, A, Leamey, C & Phillips, WD 2014, 'The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy', JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no. 94.View/Download from: Publisher's site
Abboud, M, Puglisi, DA, Davies, BN, Rybchyn, M, Whitehead, NP, Brock, KE, Cole, L, Gordon-Thomson, C, Fraser, DR & Mason, RS 2013, 'Evidence for a Specific Uptake and Retention Mechanism for 25-Hydroxyvitamin D (25OHD) in Skeletal Muscle Cells', ENDOCRINOLOGY, vol. 154, no. 9, pp. 3022-3030.View/Download from: Publisher's site
Mansour, H, McColm, JR, Cole, L, Weible, M, Korlimbinis, A & Chan-Ling, T 2013, 'Connexin 30 Expression and Frequency of Connexin Heterogeneity in Astrocyte Gap Junction Plaques Increase with Age in the Rat Retina', PLOS ONE, vol. 8, no. 3.View/Download from: Publisher's site
Song, EJ, Gordon-Thomson, C, Cole, L, Stern, H, Halliday, GM, Damian, DL, Reeve, VE & Mason, RS 2013, '1 alpha,25-Dihydroxyvitamin D-3 reduces several types of UV-induced DNA damage and contributes to photoprotection', JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 136, pp. 131-138.View/Download from: Publisher's site
Raviraj, V, Zhang, H, Chien, H-Y, Cole, L, Thompson, EW & Soon, L 2012, 'Dormant but migratory tumour cells in desmoplastic stroma of invasive ductal carcinomas', CLINICAL & EXPERIMENTAL METASTASIS, vol. 29, no. 3, pp. 273-292.View/Download from: Publisher's site
Xiang, X, Gui, H, King, NJC, Cole, L, Wang, H, Xie, Q & Bao, S 2012, 'IL-22 and non-ELR-CXC chemokine expression in chronic hepatitis B virus-infected liver', IMMUNOLOGY AND CELL BIOLOGY, vol. 90, no. 6, pp. 611-619.View/Download from: Publisher's site
Barton, DA, Cole, L, Collings, DA, Liu, DYT, Smith, PMC, Day, DA & Overall, RL 2011, 'Cell-to-cell transport via the lumen of the endoplasmic reticulum', PLANT JOURNAL, vol. 66, no. 5, pp. 806-817.View/Download from: Publisher's site
Gervasio, OL, Phillips, WD, Cole, L & Allen, DG 2011, 'Caveolae respond to cell stretch and contribute to stretch-induced signaling', JOURNAL OF CELL SCIENCE, vol. 124, no. 21, pp. 3581-3590.View/Download from: Publisher's site
Paul, NA, Cole, L, de Nys, R & Steinberg, PD 2006, 'Ultrastructure of the gland cells of the red alga Asparagopsis armata (Bonnemaisoniaceae)', JOURNAL OF PHYCOLOGY, vol. 42, no. 3, pp. 637-645.View/Download from: Publisher's site
Hyde, GJ, Davies, DS, Cole, L & Ashford, AE 2003, 'Retention of fluorescent probes during aldehyde-free anhydrous freeze-substitution', JOURNAL OF MICROSCOPY, vol. 210, pp. 125-130.View/Download from: Publisher's site
Hyde, GJ, Davies, D, Cole, L & Ashford, AE 2002, 'Regulators of GTP-binding proteins cause morphological changes in the vacuole system of the filamentous fungus, Pisolithus tinctorius', CELL MOTILITY AND THE CYTOSKELETON, vol. 51, no. 3, pp. 133-146.View/Download from: Publisher's site
Trautman, DA, Hinde, R, Cole, L, Grant, A & Quinnell, R 2002, 'Visualisation of the symbiosome membrane surrounding cnidarian algal cells', Symbiosis, vol. 32, no. 2, pp. 133-145.
Virtually nothing is known of the role of the cnidarian symbiosome, primarily due to the difficulty in visualising its membrane. We used the fluorescent dye MDY-64 to stain symbiosome membranes surrounding algae of the anthozoan Zoanthus robustus. MDY-64 did not stain cultured symbiotic dinoflagellates, confirming that this dye binds to a membrane of host cell origin. Another fluorescent dye, amino-chloromethylcoumarin (CMAC) stained the cytoplasm of both endoderm cells and algal cells from the zoanthid, a coral and an anemone. By drawing a suspension of endoderm cells from Z. robustus back and forth (5-7 times) through a hypodermic needle, we obtained approximately 73% of the algae in intact symbiosomes, with only 6% of the algae remaining in intact endoderm cells, and 21% free of both endoderm cell and symbiosome. About 15 additional passages of the cells through the needle removed the symbiosome membranes, leaving approximately 85% of the algae free of all host cell material. Use of detergents to remove the endoderm cell plasma membrane damaged both the symbiosome and algal membranes. Transmission electron microscopy showed variable numbers of membranes surrounding the algae. The ability to isolate dinoflagellate cells with and without symbiosome membranes will allow studies of the role of this membrane.
Pareek, M, Cole, L & Ashford, AE 2001, 'Variations in structure of aerial and submerged rhizomorphs of Armillaria luteobubalina indicate that they may be organs of absorption', MYCOLOGICAL RESEARCH, vol. 105, pp. 1377-1387.View/Download from: Publisher's site
Cole, L, Davies, D, Hyde, GJ & Ashford, AE 2000, 'Brefeldin A affects growth, endoplasmic reticulum, golgi bodies, tubular vacuole system, and secretory pathway in Pisolithus tinctorius', FUNGAL GENETICS AND BIOLOGY, vol. 29, no. 2, pp. 95-106.View/Download from: Publisher's site
Cole, L, Davies, D, Hyde, GJ & Ashford, AE 2000, 'ER-Tracker dye and BODIPY-brefeldin A differentiate the endoplasmic reticulum and Golgi bodies from the tubular-vacuole system in living hyphae of Pisolithus tinctorius', JOURNAL OF MICROSCOPY-OXFORD, vol. 197, pp. 239-248.
Hyde, GJ, Davies, D, Perasso, L, Cole, L & Ashford, AE 1999, 'Microtubules, but not actin microfilaments, regulate vacuole motility and morphology in hyphae of Pisolithus tinctorius', CELL MOTILITY AND THE CYTOSKELETON, vol. 42, no. 2, pp. 114-124.
Hyde, GJ, Davies, D, Perasso, L, Cole, L & Ashford, AE 1999, 'Microtubules, but not actin microfilaments, regulate vacuole motility and morphology in hyphae of Pisolithus tinctorius', Cell Motility and the Cytoskeleton, vol. 42, no. 2, pp. 114-124.View/Download from: 3.3.co;2-e">Publisher's site
Cole, L, Dewey, FM & Hawes, CR 1998, 'Immunocytochemical studies of the infection mechanisms of Botrytis fabae - II. Host cell wall breakdown', NEW PHYTOLOGIST, vol. 139, no. 4, pp. 611-622.View/Download from: Publisher's site
Cole, L, Dewey, FM & Hawes, CR 1998, 'Immunocytochemical studies of the infection mechanisms of Botrytis fabae I. The fungal extracellular matrix in penetration and post-penetration processes', NEW PHYTOLOGIST, vol. 139, no. 4, pp. 597-609.View/Download from: Publisher's site
Cole, L, Orlovich, DA & Ashford, AE 1998, 'Structure, function, and, motility of vacuoles in filamentous fungi', FUNGAL GENETICS AND BIOLOGY, vol. 24, no. 1-2, pp. 86-100.View/Download from: Publisher's site
Kearns, A, Cole, L, Hawes, CR & Evans, DE 1998, 'Establishment of low extracellular pH is essential for uptake of the fluorescent anionic dye hydroxypyrenetrisulfonate by suspension-cultured carrot cells', PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 36, no. 12, pp. 879-887.View/Download from: Publisher's site
Cole, L, Hyde, GJ & Ashford, AE 1997, 'Uptake and compartmentalisation of fluorescent probes by Pisolithus tinctorius hyphae: evidence for an anion transport mechanism at the tonoplast but not for fluid-phase endocytosis', PROTOPLASMA, vol. 199, no. 1-2, pp. 18-29.View/Download from: Publisher's site
Cole, L, Dewey, FM & Hawes, CR 1996, 'Infection mechanisms of Botrytis species: Pre-penetration and pre-infection processes of dry and wet conidia', MYCOLOGICAL RESEARCH, vol. 100, pp. 277-286.View/Download from: Publisher's site
SatiatJeunemaitre, B, Cole, L, Bourett, T, Howard, R & Hawes, C 1996, 'Brefeldin A effects in plant and fungal cells: Something new about vesicle trafficking?', JOURNAL OF MICROSCOPY-OXFORD, vol. 181, pp. 162-177.View/Download from: Publisher's site
Cole, L, Coleman, J, Kearns, A, Morgan, G & Hawes, C 1991, 'The organic anion transport inhibitor, probenecid, inhibits the transport of Lucifer Yellow at the plasma membrane and the tonoplast in suspension-cultured plant cells', Journal of Cell Science, vol. 99, no. 3, pp. 545-555.
In this paper we report on the uptake of the membrane-impermeant fluorescent probe Lucifer Yellow CH (LY-CH) into the vacuolar system of plant cell suspension cultures. LY-CH is internalised into vacuoles of maize cells at a faster 'rate' than carrot cells and in each case, the probe is also trapped at the cell wall. In the presence of the uricosuric drug probenecid, the vacuolar uptake of LY-CH by carrot and maize cells is inhibited and in some cells internalisation of probe is blocked at the plasma membrane. In electroporated carrot cells, LY-CH is sequestered slowly from the cytoplasm into vacuoles by a probenecid-inhibitable transport process. These results are compared with the effects of probenecid on the sequestration of LY-CH from the cytoplasm into the lysosomal system of fibroblasts. In view of the above findings and recent evidence for the putative uptake of LY- CH by fluid-phase endocytosis in plant cells, the possibility that LY-CH is transported across plant membranes via probenecid inhibitable organic anion transporters is discussed.
COLE, L, HAWES, C & DEWEY, FM 1991, 'IMMUNOCYTOCHEMICAL LOCALIZATION OF A SPECIFIC ANTIGEN IN CELL-WALLS OF PENICILLIUM-ISLANDICUM', MYCOLOGICAL RESEARCH, vol. 95, pp. 1369-1374.View/Download from: Publisher's site
Cole, L, Coleman, J, Evans, D & Hawes, C 1990, 'Internalisation of fluorescein isothiocyanate and fluorescein isothiocyanate-dextran by suspension-cultured plant cells', Journal of Cell Science, vol. 96, no. 4, pp. 721-730.
The uptake of pure non-conjugated fluorescein isothiocyanate (FITC) and of the membrane-impermeant probe FITC-dextran into suspension-cultured carrot cells and protoplasts has been investigated. Commercial samples of a 70K (K=103 M(r)) FITC-dextran were shown to contain contaminant FITC and/or its degradation products, which were rapidly internalised into the vacuolar system of both cells and protoplasts. However, purified samples of the 70K FITC-dextran were taken up into the vacuoles of cells but not protoplasts after a 1 h incubation period. This apparent difference in the ability of cells and protoplasts to internalise FITC-dextrans was confirmed using samples of both commercial and purified 20K FITC-dextran as putative endocytotic probes. Both confocal and conventional fluorescence microscopy of FITC-treated cells have shown that FITC was internalised into similar intracellular compartments as was observed in cells treated with three-times purified 70K FITC-dextran. Thus, FITC was a useful fluorophore for rapidly labelling both the putative endocytotic compartments and the pleiomorphic vacuolar system of carrot cells. Kinetic studies indicated that FITC entered the cell by diffusion in the form of the neutral molecule. We have shown that treatment of cells or protoplasts with the drug Probenecid reversibly inhibited the uptake of FITC from the cytoplasm into the vacuole. In addition, the uptake of FITC into isolated vacuoles was enhanced in the presence of Mg-ATP.
HAWES, C, COLEMAN, J, EVANS, D & COLE, L 1989, 'RECENT ADVANCES IN THE STUDY OF PLANT COATED VESICLES', CELL BIOLOGY INTERNATIONAL REPORTS, vol. 13, no. 1, pp. 119-128.View/Download from: Publisher's site
Chien, HY, Fok, S, Raviraj, V, Lee, J, Cole, L, Guy Lyons, J & Soon, L 2011, 'Matrix scaffolds in cancer cell biology; Physiological representation and nanoscale characterisation' in Nanomedicine and Cancer, pp. 142-159.
© 2012 by Taylor & Francis Group, LLC. All rights reserved. In vitro cultures such as 2-dimensional (D) cell culture, semi-3D, 3D, and in vivo mouse models represent sub-approximations to native conditions that had led to the development of successful translational applications. However, it is also becoming clear that not all in vivo situations are adequately represented and for a subset of patients, their disease conditions and corresponding therapeutic measures may not have been properly addressed. It is essential to match experimental approaches with in vivo environmental conditions for cellular studies. Some of these include the use of clustered cells called spheroids and single tumour cells embedded in matrices with differing densities and architecture. These configurations mimic, for example, desmoplastic tumours in breast cancers. Concomitantly, appropriate analytical tools that can accommodate samples in their native conditions and/or enable nanostructural studies are critical to evaluate the adherence of novel in vitro models to physiological conditions.
Dewey, FM & Cole, L 1996, 'Monoclonal antibody based assays for detection and quantification of Botrytis cinerea', DIAGNOSIS AND IDENTIFICATION OF PLANT PATHOGENS, 4th International Symposium of the European-Foundation-for-Plant-Pathology, SPRINGER, BONN, GERMANY, pp. 91-94.