Can supervise: YES
Chan, H, Soderstrom, B & Skoglund, U 2020, 'Spo0J and SMC are required for normal chromosome segregation in Staphylococcus aureus', MICROBIOLOGYOPEN, vol. 9, no. 4.View/Download from: Publisher's site
Soderstrom, B, Chan, H & Daley, DO 2019, 'Super-resolution images of peptidoglycan remodelling enzymes at the division site of Escherichia coli.', Current genetics, vol. 65, no. 1, pp. 99-101.View/Download from: Publisher's site
Bacterial cells need to divide. This process requires more than 30 different proteins, which gather at the division site. It is widely assumed that these proteins assemble into a macromolecular complex (the divisome), but capturing the molecular layout of this complex has proven elusive. Super-resolution microscopy can provide spatial information, down to a few tens of nanometers, about how the division proteins assemble into complexes and how their activities are co-ordinated. Herein we provide insight into recent work from our laboratories, where we used super-resolution gSTED nanoscopy to explore the molecular organization of FtsZ, FtsI and FtsN. The resulting images show that all three proteins form discrete densities organised in patchy pseudo-rings at the division site. Significantly, two-colour imaging highlighted a radial separation between FtsZ and FtsN, indicating that there is more than one type of macromolecular complex operating during division. These data provide a first glimpse into the spatial organisation of PG-synthesising enzymes during division in Gram-negative bacteria.
Soderstrom, B, Badrutdinov, A, Chan, H & Skoglund, U 2018, 'Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells', NATURE COMMUNICATIONS, vol. 9.View/Download from: Publisher's site