I am an NHMRC Peter Doherty Early Career Fellow and a core member of the Institute for Biomedical Materials and Devices (IBMD) at the University of Technology Sydney. Before joining UTS, I completed my Master Degree at the University of Ulm (Germany) and my PhD degree at the Leibniz Institute for Solid State and Materials Research Dresden (IFW Dresden e.V., Germany).
My research vision is to develop new technologies for bioanalysis and discoveries at different scales via advances in micro- and nanoscale science. I aim to develop new methods, micro-engineered materials and systems to advance biomedical research at new levels, with unmatched performance characteristics in sensitivity, selectivity, throughput, resolution and dimensions.
Specifically, my current research can be described as follows:
(1) Extracellular vesicles (EVs) represent the future generation of biomarkers. They have been found important in cell communication. Understanding the role of EVs in cell communication is critical to provide insight into disease progression, which could improve disease prediction and management. EVs are featured with significant heterogeneity, which hamper their downstream study of their biological functions and applications. This issue is compounded by the lack of capability to track their cellular origins. Towards filling this gap, my research focuses on developing new Bio-MEMS tools to enable single-cell EVs profiling.
(2) Functional microparticles have broad applications as biomolecule and cell carriers, sensors and can provide controlled microenvironments to interface with biological systems for tissue engineering and microscopy. My research in this direction focuses on synthesizing novel functional microparticles via microfluidic/lab-on-chip technologies.
My research is recognised by several honours and awards, including the NHMRC Peter Doherty Australian Biomedical Fellowship in 2018, Ehrenfried Walther von Tschirnhaus Medal in 2017, Summa Cum Laude (the highest honour) for the doctoral dissertation, a prestigious 2015 National Award for Outstanding Chinese Students Abroad.
I manage the ARC microfabrication facility at UTS, and am the associate investigator of the ARC Research Hub for IDEAL (Integrated Devices for End-user Analysis at Low levels).
Grants, Honours and Awards
- 2019 Associate Investigator, ARC Research Hub for Integrated Devices for End-user Analysis at Low levels (IDEAL)
- 2018 Rising Star Award, Australian Association of Chinese Biomedical Scientists
- 2018 NHMRC Early Career Fellowship, National Health and Medical Research Council
- 2018 UTS Research Travel Fund, University of Technology Sydney
- 2017 the Ehrenfried Walther von Tschirnhaus Medal, Leibniz Institute for Solid State and Materials Research Dresden, Germany
- 2016 Summa Cum Laude for PhD thesis, Chemnitz University of Technology, Germany
- 2015 Chinese Government Award for Outstanding Students Abroad, China Scholarship Council
- 2009 Baden-württemberg Scholarship, Baden-Württemberg Stiftung, Germany
- 2008 He Yaoguang Prize, Southeast University Education Foundation, China
- 2008 Student Research Training Project (SRTP) Excellence, School of Materials Science and Engineering, Southeast University, China
- 2006 Excellent Student, Southeast University, China
- 2006 President Prize, Southeast University, China
Reviewing services for journals
- Advanced Materials (Wiley), Advanced Materials Technologies (Wiley), Light Science and Applications (NPG), Biosensors and Bioelectronics (Elsevier), Lab on Chip (RSC), Nanoscale (RSC), Applied Physics Letter (AIP), Biomicrofluidics (AIP), Microfluidics and Nanofluidics (Springer), Scientific Reports (NPG), RSC Advances (RSC), Journal of Micromechanics and Microengineering (IOP), and many more ...
- Reviewer board member of Micromachines (MDPI)
Reviewing services for grants
- National Health and Medical Research Council (NHMRC) Ideas grant review panel
- American Chemical Society
- International Society of Advanced Cytometry
Can supervise: YES
- Microfluidics/Lab on a Chip
- Biosensors and bioelectronics for high-throughput screening and point-of-care applications
- Soft functional materials for wearable and implantable applications
- Biomedical polymers 42025 - Lecture on heterogeneous polymerizations
Fang, G, Lu, H, Law, A, Gallego-Ortega, D, Jin, D & Lin, G 2019, 'Gradient-sized control of tumor spheroids on a single chip', LAB ON A CHIP, vol. 19, no. 24, pp. 4093-4103.View/Download from: Publisher's site
Mi, C, Zhou, J, Wang, F, Lin, G & Jin, D 2019, 'Ultrasensitive Ratiometric Nanothermometer with Large Dynamic Range and Photostability', Chemistry of Materials, vol. 31, no. 22, pp. 9480-9487.View/Download from: Publisher's site
Copyright © 2019 American Chemical Society. Thermally responsive fluorescent nanoparticles can be constructed to allow robust, rapid, and noninvasive temperature measurements. Furthermore, due to their tiny size, they can be used to detect temperature changes at the nanoscale. In this way, such sensors are ideally suited to emerging applications including intracellular temperature sensing and microelectronics failure diagnostics. Despite their potential, current nanothermometers still suffer from limited sensitivity, dynamic range, and stability. By introducing thermal enhanced anti-Stokes emission from a pair of lanthanide ions, ytterbium and neodymium, we show an increase of more than 1 order of magnitude in both the sensitivity and the dynamic range when compared to conventional ytterbium and erbium-codoped nanothermometers. Here, we report heterogeneous temperature-responsive nanoparticles with a new record of sensitivity (9.6%/K at room temperature and above 2.3%/K at elevated temperatures up to 413 K) that can be used for ratiometric thermometry. The heterogeneous nanostructure design shows that the thermal responses can be fine-tuned by the controlled growth of nanoparticles. The stability of the ultrasensitive nanothermometers has enabled long-term noncontact monitoring of local heat dissipation of a microelectronic device.
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Optical nanomaterials have been widely used in anticounterfeiting applications. There have been significant developments powered by recent advances in material science, printing technologies, and the availability of smartphone-based decoding technology. Recent progress in this field is surveyed, including the availability of optical reflection, absorption, scattering, and luminescent nanoparticles. It is demonstrated that advances in the design and synthesis of lanthanide-doped upconversion nanoparticles will lead to the next generation of anticounterfeiting technologies. Their tunable optical properties and optical responses to a range of external stimuli allow high-security level information encoding. Challenges in the scale-up synthesis of nanomaterials, engineering of assessorial devices for smart-phone-based decryption, and alignment to the potential markets which will lead to new directions for research, are discussed.
He, H, Howard, CB, Chen, Y, Wen, S, Lin, G, Zhou, J, Thurecht, KJ & Jin, D 2018, 'Bispecific Antibody-Functionalized Upconversion Nanoprobe.', Analytical chemistry, vol. 90, no. 5, pp. 3024-3029.View/Download from: UTS OPUS or Publisher's site
Upconversion nanoparticles (UCNPs) are new optical probes for biological applications. For specific biomolecular recognition to be realized for diagnosis and imaging, the key lies in developing a stable and easy-to-use bioconjugation method for antibody modification. Current methods are not yet satisfactory regarding conjugation time, stability, and binding efficiency. Here, we report a facile and high-yield approach based on a bispecific antibody (BsAb) free of chemical reaction steps. One end of the BsAb is designed to recognize methoxy polyethylene glycol-coated UCNPs, and the other end of the BsAb is designed to recognize the cancer antigen biomarker. Through simple vortexing, BsAb-UCNP nanoprobes form within 30 min and show higher (up to 54%) association to the target than that of the traditional UCNP nanoprobes in the ELISA-like assay. We further demonstrate its successful binding to the cancer cells with high efficiency and specificity for background-free fluorescence imaging under near-infrared excitation. This method suggests a general approach broadly suitable for functionalizing a range of nanoparticles to specifically target biomolecules.
He, H, Liu, B, Wen, S, Liao, J, Lin, G, Zhou, J & Jin, D 2018, 'Quantitative Lateral Flow Strip Sensor Using Highly Doped Upconversion Nanoparticles.', Analytical chemistry, vol. 90, no. 21, pp. 12356-12360.View/Download from: UTS OPUS or Publisher's site
Paper-based lateral flow assays, though being low-cost and widely used for rapid in vitro diagnostics, are indicative and do not provide sufficient sensitivity for the detection and quantification of low abundant biomarkers for early stage cancer diagnosis. Here, we design a compact device to create a focused illumination spot with high irradiance, which activates a range of highly doped 50 nm upconversion nanoparticles (UCNPs) to produce orders of magnitude brighter emissions. The device employs a very low-cost laser diode, simplified excitation, and collection optics and permits a mobile phone camera to record the results. Using highly erbium ion (Er3+)-doped and thulium ion (Tm3+)-doped UCNPs as two independent reporters on two-color lateral flow strips, new records of limit of detection (LOD), 89 and 400 pg/mL, have been achieved for the ultrasensitive detection of prostate specific antigen (PSA) and ephrin type-A receptor 2 (EphA2) biomarkers, respectively, without crosstalk. The technique and device presented in this work suggests a broad scope of low-cost, rapid, and quantitative lateral flow assays in early detection of bioanalytes.
Ren, W, Wen, S, Tawfik, SA, Su, QP, Lin, G, Ju, LA, Ford, MJ, Ghodke, H, van Oijen, AM & Jin, D 2018, 'Anisotropic functionalization of upconversion nanoparticles.', Chemical science, vol. 9, no. 18, pp. 4352-4358.View/Download from: UTS OPUS or Publisher's site
Despite significant advances toward accurate tuning of the size and shape of colloidal nanoparticles, the precise control of the surface chemistry thereof remains a grand challenge. It is desirable to conjugate functional bio-molecules onto the selected facets of nanoparticles owing to the versatile capabilities rendered by the molecules. We report here facet-selective conjugation of DNA molecules onto upconversion nanoparticles via ligand competition reaction. Different binding strengths of phosphodiester bonds and phosphate groups on DNA and the surfactant molecules allow one to create heterogeneous bio-chemistry surface for upconversion nanoparticles. The tailored surface properties lead to the formation of distinct self-assembly structures. Our findings provide insight into the interactions between biomolecules and nanoparticles, unveiling the potential of using nanoparticles as fundamental building blocks for creating self-assembled nano-architectures.
Ren, W, Zhou, Y, Lin, G, Wen, S, He, H, Liu, D & Jin, D 2018, 'DNA-mediated Anisotropic Silica Coating of Upconversion Nanoparticles', Chemical Communications, vol. 54, no. 52.View/Download from: UTS OPUS or Publisher's site
Lin, G, Makarov, D & Schmidt, OG 2017, 'Magnetic sensing platform technologies for biomedical applications.', Lab on a Chip, vol. 17, no. 11, pp. 1884-1912.View/Download from: UTS OPUS or Publisher's site
Detection and quantification of a variety of micro- and nanoscale entities, e.g. molecules, cells, and particles, are crucial components of modern biomedical research, in which biosensing platform technologies play a vital role. Confronted with the drastic global demographic changes, future biomedical research entails continuous development of new-generation biosensing platforms targeting even lower costs, more compactness, and higher throughput, sensitivity and selectivity. Among a wide choice of fundamental biosensing principles, magnetic sensing technologies enabled by magnetic field sensors and magnetic particles offer attractive advantages. The key features of a magnetic sensing format include the use of commercially available magnetic field sensing elements, e.g. magnetoresistive sensors which bear huge potential for compact integration, a magnetic field sensing mechanism which is free from interference by complex biomedical samples, and an additional degree of freedom for the on-chip handling of biochemical species rendered by magnetic labels. In this review, we highlight the historical basis, routes, recent advances and applications of magnetic biosensing platform technologies based on magnetoresistive sensors.
Miao, S, He, S, Liang, M, Lin, G, Cai, B & Schmidt, OG 2017, 'Microtubular Fuel Cell with Ultrahigh Power Output per Footprint.', Advanced Materials, vol. 29, no. 34.View/Download from: UTS OPUS or Publisher's site
A novel realization of microtubular direct methanol fuel cells (µDMFC) with ultrahigh power output is reported by using "rolled-up" nanotechnology. The microtube (Pt-RuO2 -RUMT) is prepared by rolling up Ru2 O layers coated with magnetron-sputtered Pt nanoparticles (cat-NPs). The µDMFC is fabricated by embedding the tube in a fluidic cell. The footprint of per tube is as small as 1.5 × 10-4 cm2 . A power density of ≈257 mW cm-2 is obtained, which is three orders of magnitude higher than the present microsized DFMCs. Atomic layer deposition technique is applied to alleviate the methanol crossover as well as improve stability of the tube, sustaining electrolyte flow for days. A laminar flow driven mechanism is proposed, and the kinetics of the fuel oxidation depends on a linear-diffusion-controlled process. The electrocatalytic performance on anode and cathode is studied by scanning both sides of the tube wall as an ex situ working electrode, respectively. This prototype µDFMC is extremely interesting for integration with micro- and nanoelectronics systems.
Song, W, Lin, G, Ge, J, Fassbender, J & Makarov, D 2017, 'Encoding Microreactors with Droplet Chains in Microfluidics.', ACS Sensors, vol. 2, no. 12, pp. 1839-1846.View/Download from: UTS OPUS or Publisher's site
Droplet-based high throughput biomolecular screening and combinatorial synthesis entail a viable indexing strategy to be developed for the identification of each microreactor. Here, we propose a novel indexing scheme based on the generation of droplet sequences on demand to form unique encoding droplet chains in fluidic networks. These codes are represented by multiunit and multilevel droplets packages, with each code unit possessing several distinct signal levels, potentially allowing large encoding capacity. For proof of concept, we use magnetic nanoparticles as the encoding material and a giant magnetoresistance (GMR) sensor-based active sorting system supplemented with an optical detector to generate and decode the sequence of one exemplar sample droplet reactor and a 4-unit quaternary magnetic code. The indexing capacity offered by 4-unit multilevel codes with this indexing strategy is estimated to exceed 104, which holds great promise for large-scale droplet-based screening and synthesis.
Lin, G, Karnaushenko, DD, Bermudez, GSC, Schmidt, OG & Makarov, D 2016, 'Magnetic Suspension Array Technology: Controlled Synthesis and Screening in Microfluidic Networks', SMALL, vol. 12, no. 33, pp. 4553-4562.View/Download from: Publisher's site
Lin, G, Karnaushenko, DD, Bermúdez, GSC, Schmidt, OG & Makarov, D 2016, 'Droplet Microfluidics: Magnetic Suspension Array Technology: Controlled Synthesis and Screening in Microfluidic Networks (Small 33/2016)', Small, vol. 12, no. 33, p. 4580.View/Download from: Publisher's site
Karnaushenko, D, Ibarlucea, B, Lee, S, Lin, G, Baraban, L, Pregl, S, Melzer, M, Makarov, D, Weber, WM, Mikolajick, T, Schmidt, OG & Cuniberti, G 2015, 'Flexible Electronics: Light Weight and Flexible High-Performance Diagnostic Platform (Adv. Healthcare Mater. 10/2015)', Advanced Healthcare Materials, vol. 4, no. 10, pp. 1419-1419.View/Download from: Publisher's site
Karnaushenko, D, Ibarlucea, B, Lee, S, Lin, G, Baraban, L, Pregl, S, Melzer, M, Makarov, D, Weber, WM, Mikolajick, T, Schmidt, OG & Cuniberti, G 2015, 'Light Weight and Flexible High-Performance Diagnostic Platform', ADVANCED HEALTHCARE MATERIALS, vol. 4, no. 10, pp. 1517-1525.View/Download from: Publisher's site
Lin, G, Fomin, VM, Makarov, D & Schmidt, OG 2015, 'Supervised discriminant analysis for droplet micro-magnetofluidics', MICROFLUIDICS AND NANOFLUIDICS, vol. 19, no. 2, pp. 457-464.View/Download from: Publisher's site
Lin, G, Makarov, D & Schmidt, OG 2015, 'Strong Ferromagnetically-Coupled Spin Valve Sensor Devices for Droplet Magnetofluidics', SENSORS, vol. 15, no. 6, pp. 12526-12538.View/Download from: Publisher's site
Lin, G, Makarov, D, Medina-Sanchez, M, Guix, M, Baraban, L, Cuniberti, G & Schmidt, OG 2015, 'Magnetofluidic platform for multidimensional magnetic and optical barcoding of droplets', LAB ON A CHIP, vol. 15, no. 1, pp. 216-224.View/Download from: Publisher's site
Melzer, M, Karnaushenko, D, Lin, G, Baunack, S, Makarov, D & Schmidt, OG 2015, 'Direct Transfer of Magnetic Sensor Devices to Elastomeric Supports for Stretchable Electronics', ADVANCED MATERIALS, vol. 27, no. 8, pp. 1333-+.View/Download from: Publisher's site
Melzer, M, Karnaushenko, D, Lin, G, Baunack, S, Makarov, D & Schmidt, OG 2015, 'Stretchable Electronics: Direct Transfer of Magnetic Sensor Devices to Elastomeric Supports for Stretchable Electronics (Adv. Mater. 8/2015)', Advanced Materials, vol. 27, no. 8, pp. 1306-1306.View/Download from: Publisher's site
Streubel, R, Han, L, Im, M-Y, Kronast, F, Roessler, UK, Radu, F, Abrudan, R, Lin, G, Schmidt, OG, Fischer, P & Makarov, D 2015, 'Manipulating Topological States by Imprinting Non-Collinear Spin Textures', SCIENTIFIC REPORTS, vol. 5.View/Download from: Publisher's site
Lin, G, Makarov, D, Melzer, M, Si, W, Yan, C & Schmidt, OG 2014, 'A highly flexible and compact magnetoresistive analytic device', LAB ON A CHIP, vol. 14, no. 20, pp. 4050-4058.View/Download from: Publisher's site
Si, W, Moench, I, Yan, C, Deng, J, Li, S, Lin, G, Han, L, Mei, Y & Schmidt, OG 2014, 'A Single Rolled-Up Si Tube Battery for the Study of Electrochemical Kinetics, Electrical Conductivity, and Structural Integrity', ADVANCED MATERIALS, vol. 26, no. 47, pp. 7973-+.View/Download from: Publisher's site
Melzer, M, Lin, G, Makarov, D & Schmidt, OG 2012, 'Stretchable Spin Valves on Elastomer Membranes by Predetermined Periodic Fracture and Random Wrinkling', Advanced Materials, vol. 24, no. 48, pp. 6468-6472.View/Download from: UTS OPUS or Publisher's site
The first highly stretchable and sensitive spin valve sensor on elastomeric membranes are demonstrated. The sensor elements exhibit stable GMR behavior up to tensile strains of 29% in in situ stretching experiments and show no fatigue over 500 loading cycles. This remarkable stretchability is achieved by a predetermined periodic fracture mechanism that creates a meander-like pattern upon stretching.
Application fields such as smart skin1 and flexible or stretchable consumer electronics2–6 require a wide range of electronic components that are shapeable into nonplanar geometries after fabrication. Ideally, they should also be elastic and withstand many cycles of deformations without degrading in performance. Mechanical compliancy of thin films of naturally stiff materials can be achieved by different morphology transitions,6 that transfer large deformations of the substrate into small strains in the functional film, e.g., by wrinkling7, 8 or lithographically defined meander structures.9, 10 However, exploiting the full bandwidth and application potential of modern electronics requires implementation of stretchability into all types of electronic devices.
For instance, there is rapid progress in fabrication of interconnects11 and opto-electronic devices12 with stretchabilities up to several tens of percents. During the last years, magnetic13, 14 as well as magnetoresistive15–18 structures were fabricated and characterized on bendable polymeric substrates. Recently, stretchable magneto-electronics has been introduced by the fabrication of a wrinkled magnetic sensor based on [Co(Py)/Cu] multilayers19, 20 revealing a giant magnetoresistive (GMR) effect; Py stands for the permalloy, Ni81Fe19 alloy. However, only moderate stretchability of the magnetic sensor of up to about 4% was achieved. Furthermore, the sensitivity of stretchable magnetic sensors to small magnetic fields, as required for applications such as wearable electronics,1 has to be substantially enhanced....
Makarov, D, Melzer, M, Karnaushenko, D, Lin, G, Monch, I & Schmidt, OG 2015, 'Shapeable magnetic sensorics', 2015 IEEE International Magnetics Conference, INTERMAG 2015.View/Download from: Publisher's site
© 2015 IEEE. The flourishing and eagerness of portable consumer electronics necessitates functional elements to be lightweight, flexible, and even wearable (Figure 1) , fulfilling the needs of soft robotics, medical implants, imperceptible and transient electronics [2-4]. Next generation flexible appliances aim to become fully autonomous and will require ultra-thin and flexible navigation modules, body tracking and relative position monitoring systems. Key building blocks of navigation and position tracking devices are the magnetic field sensors.
Nickel, F, Gottlob, DM, Krug, IP, Doganay, H, Cramm, S, Kaiser, AM, Lin, G, Makarov, D, Schmidt, OG & Schneider, CM 2012, 'Time-resolved magnetic imaging in an aberration-corrected, energy-filtered photoemission electron microscope', ULTRAMICROSCOPY, 8th International Workshop on Low Energy Electron Microscopy and Photoemission Electron Microscopy (LEEM/PEEM), ELSEVIER SCIENCE BV, Hong Kong, PEOPLES R CHINA, pp. 54-62.View/Download from: Publisher's site
Minomic International Ltd. for prostate cancer diagnostics