Professor Dianne Jolley joined UTS as Dean of the Faculty of Science in December 2018.
Dianne is an environmental chemist and toxicologist with expertise in senior management, executive boards, and industry engagement, with experience in project management in STEMM research and teaching, collaborating with multidisciplinary partners in government, industry and higher education.
Dianne’s research contributes to understanding contaminant toxicity and the development of environmental guidelines that assist in the regulation and remediation of contamination. For Dianne, and the over 40 research students she has supervised in her career, national and international collaborations with partners in academia, industry and government are critical to achieving research outcomes and impact. In 2016 Dianne was awarded the Environment Medal from the Royal Australian Chemical Institute (RACI), was recognised as a Women of Impact, and was awarded the Vice Chancellor’s award for Excellence in Research Supervision at the University of Wollongong. Dianne was a member of a team awarded the 2018 Outstanding Achievement in Research Partnership and Impact, Vice Chancellors Award (for sustained Antarctic Research between the UOW with the Australian Antarctic Division and CSIRO). Dianne is a fellow of Royal Australian Chemical Institute, member of Science Technology Australia, and the international Society of Environmental Toxicology and Chemistry.
Can supervise: YES
Ngo, LK, Price, HL, Bennett, WW, Teasdale, PR & Jolley, DF 2020, 'DGT and selective extractions reveal differences in arsenic and antimony uptake by the white icicle radish (Raphanus sativus)', Environmental Pollution, vol. 259.View/Download from: UTS OPUS or Publisher's site
© 2019 Increasing soil contamination of arsenic (As) and antimony (Sb) is posing a serious concern to human health. Due to insufficient studies on Sb, the biogeochemical behaviour and plant uptake of Sb are assumed to be similar to that of As. As part of extensive research unravelling As and Sb biogeochemistry and plant uptake, the diffusive gradients in thin films (DGT) technique and sequential extraction procedure (SEP) were applied to evaluate As and Sb uptake by the white icicle radish (Raphanus sativus) cultivated in diluted cattle dip soils contaminated with As only and diluted mining soils contaminated with both As and Sb under agricultural conditions. Labile As and Sb in these soils measured by DGT (CDGT), soil solution (Csol), and SEP (CSEP-labile), were compared with As and Sb bioaccumulation in R. sativus tissues. Regardless of contamination sources and measurement techniques, the results showed that As was consistently more labile than Sb although total As concentrations in two soil types were lower than total Sb. Labile As in cattle dip soils was higher than that in mining soils, although there were no significant differences in soil As concentrations. The analysis of R. sativus tissues revealed that the overall As bioaccumulation was 4.5-fold higher than for Sb, and that As translocation to shoots was limited. In contrast, considerable Sb translocation to shoots was observed. The As and Sb bioaccumulation were strongly correlated with their CSEP-labile, CDGT, and Csol (R2 = 0.87–0.99), demonstrating the effectiveness of these techniques in predicting As and Sb in the white icicle radish. Compared with the cherry bell radish previously studied, the white icicle radish exhibited higher bioaccumulation factors (BAF) for Sb, but lower BAF for As, and lower translocation of As and Sb to shoots, providing understanding of how As and Sb are accumulated by radish cultivars.
Gissi, F, Reichelt-Brushett, AJ, Chariton, AA, Stauber, JL, Greenfield, P, Humphrey, C, Salmon, M, Stephenson, SA, Cresswell, T & Jolley, DF 2019, 'The effect of dissolved nickel and copper on the adult coral Acropora muricata and its microbiome.', Environmental pollution (Barking, Essex : 1987), vol. 250, pp. 792-806.View/Download from: UTS OPUS or Publisher's site
The potential impacts of mining activities on tropical coastal ecosystems are poorly understood. In particular, limited information is available on the effects of metals on scleractinian corals which are foundation species that form vital structural habitats supporting other biota. This study investigated the effects of dissolved nickel and copper on the coral Acropora muricata and its associated microbiota. Corals collected from the Great Barrier Reef were exposed to dissolved nickel (45, 90, 470, 900 and 9050 μg Ni/L) or copper (4, 11, 32 and 65 μg Cu/L) in flow through chambers at the National Sea Simulator, Townsville, Qld, Australia. After a 96-h exposure DNA metabarcoding (16S rDNA and 18S rDNA) was undertaken on all samples to detect changes in the structure of the coral microbiome. The controls remained healthy throughout the study period. After 36 h, bleaching was only observed in corals exposed to 32 and 65 μg Cu/L and very high nickel concentrations (9050 μg Ni/L). At 96 h, significant discolouration of corals was only observed in 470 and 900 μg Ni/L treatments, the highest concentrations tested. While high concentrations of nickel caused bleaching, no changes in the composition of their microbiome communities were observed. In contrast, exposure to copper not only resulted in bleaching, but altered the composition of both the eukaryote and bacterial communities of the coral's microbiomes. Our findings showed that these effects were only evident at relatively high concentrations of nickel and copper, reflecting concentrations observed only in extremely polluted environments. Elevated metal concentrations have the capacity to alter the microbiomes which are inherently linked to coral health.
Koppel, DJ, Adams, MS, King, CK & Jolley, DF 2019, 'Diffusive Gradients in Thin Films Can Predict the Toxicity of Metal Mixtures to Two Microalgae: Validation for Environmental Monitoring in Antarctic Marine Conditions.', Environmental toxicology and chemistry, vol. 38, no. 6, pp. 1323-1333.View/Download from: UTS OPUS or Publisher's site
Anthropogenic contamination in the Antarctic near-shore marine environment is a challenge for environmental managers because of its isolation, high costs associated with monitoring and remediation activities, and the current lack of Antarctic-specific ecotoxicological data. The present study investigated the application of diffusive gradients in thin films (DGT) with a Chelex-100 binding resin for metal contaminant assessment in Antarctic marine conditions. Diffusion coefficients for cadmium (Cd), copper (Cu), nickel (Ni), lead (Pb), and zinc (Zn), determined at 1 °C, ranged between 2.1 and 2.6×10-6 cm2 /s and were up to 32% lower than those derived by theoretical calculations. Competition of metals on the DGT binding resin was observed at subsaturation concentrations, reducing the effective capacity for metal uptake by approximately 60%. The lability of the dissolved (0.45 µm filterable) Cd, Cu, Pb, and Zn metal fraction to DGT was generally >90% and unaffected by the presence of the Antarctic marine microalga Phaeocystis antarctica. Both DGT and dissolved metal concentrations gave equivalent mixture toxicity predictions in independent action and concentration addition models to P. antarctica and Cryothecomonas armigera; that is, predictions using DGT-labile concentrations also showed antagonism to P. antarctica, which agrees with previously determined mixture interactivity. The benefits of DGT over traditional sampling techniques (i.e., discrete water sampling) include lower method detection limits (MDLs), in situ assessment, and time-averaged concentrations which capture pulses of contamination typical of the Antarctic near-shore marine environment. The present study provides MDLs and recommended minimum deployment times to guide field deployments in Antarctica. Environ Toxicol Chem 2019;38:1323-1333. © 2019 SETAC.
Koppel, DJ, Adams, MS, King, CK & Jolley, DF 2019, 'Preliminary study of cellular metal accumulation in two Antarctic marine microalgae - implications for mixture interactivity and dietary risk.', Environmental pollution (Barking, Essex : 1987), vol. 252, no. Pt B, pp. 1582-1592.View/Download from: UTS OPUS or Publisher's site
Localised sites in Antarctica are contaminated with mixtures of metals, yet the risk this contamination poses to the marine ecosystem is not well characterised. Recent research showed that two Antarctic marine microalgae have antagonistic responses to a mixture of five common metals (Koppel et al., 2018a). However, the metal accumulating potential and risk to secondary consumers through dietary exposure are still unknown. This study investigates cellular accumulation following exposure to a mixture of cadmium, copper, nickel, lead, and zinc for the Antarctic marine microalgae, Phaeocystis antarctica and Cryothecomonas armigera. In both microalgae, cellular cadmium, copper, and lead concentrations increased with increasing exposures while cellular nickel and zinc did not. For both microalgae, copper in the metal mixture drives inhibition of growth rate with R2 values > -0.84 for all cellular fractions in both species and the observed antagonism was likely caused by zinc competition, having significantly positive partial regressions. Metal accumulation to P. antarctica and C. armigera is likely to be toxic to consumer organisms, with low exposure concentrations resulting in cellular concentrations of 500 and 1400 × 10-18 mol Zn cell-1 and 160 and 320 × 10-18 mol Cu cell-1, respectively.
Koppel, DJ, King, CK, Brown, KE, Price, GAV, Adams, MS & Jolley, DF 2019, 'Assessing the Risk of Metals and Their Mixtures in the Antarctic Nearshore Marine Environment with Diffusive Gradients in Thin-Films', Environmental Science and Technology.View/Download from: UTS OPUS or Publisher's site
© 2019 American Chemical Society. Robust environmental assessments and contaminant monitoring in Antarctic near-shore marine environments need new techniques to overcome challenges presented by a highly dynamic environment. This study outlines an approach for contaminant monitoring and risk assessment in Antarctic marine conditions using diffusive gradients in thin-films (DGT) coupled to regionally specific ecotoxicology data and environmental quality standards. This is demonstrated in a field study where DGT samplers were deployed in the near-shore marine environment of East Antarctica around the operational Casey station and the abandoned Wilkes station to measure the time-averaged biologically available fraction of metal contaminants. The incorporation of DGT-labile concentrations to reference toxicity mixture models for three Antarctic organisms predicted low toxic effects (<5% effect to the growth or development of each organism). The comparison of metal concentrations to the Australian and New Zealand default water quality guideline values (WQGVs) showed no marine site exceeding the WQGVs for 95% species protection. However, all sites exceeded the 99% WQGVs due to copper concentrations that are likely of geogenic origin (i.e., not from anthropogenic sources). This study provides evidence supporting the use of the DGT technique to monitor contaminants and assess their environmental risk in the near-shore marine environment of Antarctica.
Long, M, Holland, A, Planquette, H, González Santana, D, Whitby, H, Soudant, P, Sarthou, G, Hégaret, H & Jolley, DF 2019, 'Effects of copper on the dinoflagellate Alexandrium minutum and its allelochemical potency.', Aquatic toxicology (Amsterdam, Netherlands), vol. 210, pp. 251-261.View/Download from: UTS OPUS or Publisher's site
The dinoflagellate Alexandrium minutum produces toxic compounds, including paralytic shellfish toxins, but also some unknown extracellular toxins. Although copper (Cu) is an essential element, it can impair microalgal physiology and increase their toxic potency. This study investigated the effect of different concentrations of dissolved Cu (7 nM, 79 nM and 164 nM) on A. minutum allelochemical potency, here defined as negative effects of a protist on competing protists through the release of chemicals. This was studied in relation to its physiology. The effects of Cu were assessed on A. minutum growth, reactive oxygen species level, photosynthesis proxies, lipid metabolism, exudation of dissolved organic compounds, allelochemical potency and on the associate free bacterial community of A. minutum. Only the highest Cu exposure (164 nM) inhibited and delayed the growth of A. minutum, and only in this treatment did the allelochemical potency significantly increase, when the dissolved Cu concentration was still toxic. Within the first 7 days of the high Cu treatment, the physiology of A. minutum was severely impaired with decreased growth and photosynthesis, and increased stress responses and free bacterial density per algal cell. After 15 days, A. minutum partially recovered from Cu stress as highlighted by the growth rate, reactive oxygen species level and photosystem II yields. This recovery could be attributed to the apparent decrease in background dissolved Cu concentration to a non-toxic level, suggesting that the release of exudates may have partially decreased the bioavailable Cu fraction. Overall, A. minutum appeared quite tolerant to Cu, and this work suggests that the modifications in the physiology and in the exudates help the algae to cope with Cu exposure. Moreover, this study shows the complex interplay between abiotic and biotic factors that can influence the dynamic of A. minutum blooms. Modulation in allelochemical potency of A. minutum by Cu may hav...
Macoustra, G, Holland, A, Stauber, J & Jolley, DF 2019, 'Effect of Various Natural Dissolved Organic Carbon on Copper Lability and Toxicity to the Tropical Freshwater Microalga Chlorella sp.', Environmental science & technology, vol. 53, no. 5, pp. 2768-2777.View/Download from: UTS OPUS or Publisher's site
This study adds further critical information to the limited body of knowledge on the ameliorative ability of Australian dissolved organic carbon (DOC), reinforcing the importance of DOC source and concentration as significant factors controlling the risk copper poses to organisms in freshwater systems. The ameliorative ability of five unstudied DOCs on the chronic toxicity of copper to the tropical alga Chlorella sp. was compared. Sensitivity to copper varied dramatically; effect concentrations at the 50 percent effect level (EC50) increased by up to 22-fold in the high DOC treatment compared to controls and more than 2-fold between DOCs at the same concentration. The analytical techniques, diffusive gradients in thin films (DGT) and Chelex column, were used to understand whether differences in copper toxicity could be explained by copper lability. Labile copper mirrored the trends seen in the toxicity tests; lability decreased with increasing DOC concentration and varied between DOCs at the same concentration. The equilibrium model, WHAM VII, was also used to better understand the role of the free copper ion on the toxicity observed. Disagreement between EC50 values derived using the WHAM-predicted free Cu2+ concentrations and agreement between DGT-labile and the maximum dynamic concentration ( cmaxdyn) suggest free copper is not the sole contributor to toxicity and that the source of the specific DOCs also plays a role.
Stone, S, Adams, MS, Stauber, JL, Jolley, DF & Warne, MSJ 2019, 'Development and application of a multispecies toxicity test with tropical freshwater microalgae.', Environmental pollution (Barking, Essex : 1987), vol. 250, pp. 97-106.View/Download from: UTS OPUS or Publisher's site
Microalgae are commonly used in ecotoxicity testing due to their ease of culturing and rapid cell division rates. These tests generally utilise a single species of algae; however, microalgae occur in the environment as complex communities of multiple species. To date, routine multispecies toxicity tests using tropical microalgae have not been available. This study investigated four tropical freshwater microalgal species for use in a chronic multispecies toxicity test based on the population growth (cell division) rate: Pediastrum duplex, Monoraphidium arcuatum, Nannochloropsis-like sp. and Chlorella sp. 12. Flow cytometric analysis identified the different fluorescence and light scattering properties of each algal species and quantified each species within multispecies mixtures. Following optimisation of test media nutrients and pH, a toxicity testing protocol was developed with P. duplex, M. arcuatum and Nannochloropsis-like sp. There were no significant differences in growth rates of each alga when tested over 72 h as single species or in multispecies mixtures. Atrazine and imazapic, two herbicides with different modes of action, were used to assess the sensitivity of the multispecies toxicity test. Atrazine was toxic to all species with 72-h IC10 values of 7.2, 63 and 280 μg/L for P. duplex, M. arcuatum and Nannochloropsis-like sp. respectively, while imazapic was not toxic to any species at concentrations up to 1100 μg/L. The toxicity of atrazine and imazapic to each microalgal species in the multispecies toxicity test was the same as that determined from single-species toxicity tests indicating that the presence of these microalgae in a mixture did not affect the toxicity of these two herbicides. This study is the first to develop a multispecies tropical microalgal toxicity test for application in freshwaters. This time- and cost-effective tool can be utilised to generate data to assist environmental decision making and to undertake risk assessments of conta...
Gaw, S, Harford, A, Pettigrove, V, Sevicke-Jones, G, Manning, T, Ataria, J, Cresswell, T, Dafforn, KA, Leusch, FDL, Moggridge, B, Cameron, M, Chapman, J, Coates, G, Colville, A, Death, C, Hageman, K, Hassell, K, Hoak, M, Gadd, J, Jolley, DF, Karami, A, Kotzakoulakis, K, Lim, R, McRae, N, Metzeling, L, Mooney, T, Myers, J, Pearson, A, Saaristo, M, Sharley, D, Stuthe, J, Sutherland, O, Thomas, O, Tremblay, L, Wood, W, Boxall, ABA, Rudd, MA & Brooks, BW 2019, 'Towards Sustainable Environmental Quality: Priority Research Questions for the Australasian Region of Oceania', INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT, vol. 15, no. 6, pp. 917-935.View/Download from: Publisher's site
Amato, ED, Marasinghe Wadige, CPM, Taylor, AM, Maher, WA, Simpson, SL & Jolley, DF 2018, 'Field and laboratory evaluation of DGT for predicting metal bioaccumulation and toxicity in the freshwater bivalve Hyridella australis exposed to contaminated sediments.', Environmental pollution (Barking, Essex : 1987), vol. 243, no. Pt B, pp. 862-871.View/Download from: UTS OPUS or Publisher's site
The diffusive gradients in thin films (DGT) technique has shown to be a useful tool for predicting metal bioavailability and toxicity in sediments, however, links between DGT measurements and biological responses have often relied on laboratory-based exposures and further field evaluations are required. In this study, DGT probes were deployed in metal-contaminated (Cd, Pb, Zn) sediments to evaluate relationships between bioaccumulation by the freshwater bivalve Hyridella australis and DGT-metal fluxes under both laboratory and field conditions. The DGT-metal flux measured across the sediment/water interface (±1 cm) was useful for predicting significant cadmium and zinc bioaccumulation, irrespective of the type of sediment and exposure. A greater DGT-Zn flux measured in the field was consistent with significantly higher zinc bioaccumulation, highlighting the importance of performing metal bioavailability assessments in situ. In addition, DGT fluxes were useful for predicting the potential risk of sub-lethal toxicity (i.e., lipid peroxidation and lysosomal membrane damage). Due to its ability to account for multiple metal exposures, DGT better predicted bioaccumulation and toxicity than particulate metal concentrations in sediments. These results provide further evidence supporting the applicability of the DGT technique as a monitoring tool for sediment quality assessment.
Angel, BM, Goodwyn, K, Jolley, DF & Simpson, SL 2018, 'The use of time-averaged concentrations of metals to predict the toxicity of pulsed complex effluent exposures to a freshwater alga.', Environmental pollution (Barking, Essex : 1987), vol. 238, pp. 607-616.View/Download from: UTS OPUS or Publisher's site
Intermittent, fluctuating and pulsed contaminant discharges may result in organisms receiving highly variable toxicant exposures. This study investigated the toxicity of continuous and pulsed exposures of a complex, neutralised drainage water (NDW) and dissolved copper-spiked dilute NDW to the green alga, Pseudokirchneriella subcapitata. The effects of single pulses of between 1 and 48 h duration and continuous exposures (72 h) on algal growth rate inhibition were compared on a time-averaged concentration (TAC) basis. Algal growth rates generally recovered to control levels within 24-48 h of the pulse removal. Continuous exposures to NDW resulted in similar or marginally higher toxicity to the algae when compared to pulsed exposures of equivalent TAC (% NDW). The toxicity of the NDW was attributed mostly to the metals, with the major cations potentially causing effects that are both additive (direct toxicity) and antagonistic (lower bioavailability of trace metals). For dissolved copper in dilute NDW, the pulsed exposures caused slightly higher toxicity than continuous exposures of equivalent dissolved copper TAC, with much of the difference explained by differences in labile copper concentrations between treatments. The results indicate that water quality guideline values for toxicants derived from continuous chronic exposures may be relaxed for pulsed exposures by a factor related to the TAC with the intent to provide an adequately protective but not overly-conservative outcome. The study highlights the influence that natural water quality parameters such as water hardness and DOC can have metal speciation and toxicity, and indicates that these parameters are particularly important for site-specific water quality guideline value derivation where, on a TAC basis, pulsed exposures may be more toxic than continuous exposures typically used in guideline value derivation.
Egodawatta, LP, Macoustra, GK, Ngo, LK & Jolley, DF 2018, 'As and Sb are more labile and toxic to water spinach (Ipomoea aquatica) in recently contaminated soils than historically co-contaminated soils.', Environmental science. Processes & impacts, vol. 20, no. 5, pp. 833-844.View/Download from: UTS OPUS or Publisher's site
Elevated concentrations of As and Sb impact environmental quality and human health. In this study total and bioavailable As and Sb were measured from recently and historically contaminated soils and the phytotoxicity of these soils was evaluated with Ipomoea aquatica (35-d exposure from germination) using biomass, length of plant tissues and photosynthetic efficiency. As and Sb were both present within the soil (co-contaminated). The bioavailable As and Sb in soils were determined by a Sequential Extraction Procedure (SEP) and compared to total soil concentrations and bioaccumulation in the edible parts of I. aquatica. For both As and Sb, bioavailable concentrations increased proportionally with the total soil concentrations and greater bioavailability in recently contaminated soil was observed. Tissue dry mass and length drastically reduced with increasing total and SEP-bioavailable As and Sb soil concentrations. The total soil concentration was a less sensitive measure of the phytotoxicity of As and Sb than the bioavailable fraction. Shoot length was inhibited by 50% (EC50) at bioavailable As concentrations of 80-96 mg kg-1 in both recently and historically contaminated soils; however, bioavailable Sb EC50 for shoot length was achieved at lower bioavailable concentrations, 96 (42-219) and 12 (7-19) mg kg-1 in recently contaminated soils and historically contaminated soils, respectively. Shoot biomass was inhibited by 50% (EC50) at bioavailable As concentrations of 11 (4-30) and 49 (37-65) mg kg-1 in recently and historically contaminated soils, respectively whereas this occurred at much lower bioavailable Sb concentrations, 2-5 mg kg-1 in both recently and historically contaminated soils. Aging is important in contaminated soils, it decreases the lability of As and Sb and hence their bioavailability to agricultural plants, thus posing a lower risk of exposure of these metalloids to humans through agricultural plants grown in contaminated soils.
Enge, TG, Ecroyd, H, Jolley, DF, Yerbury, JJ, Kalmar, B & Dosseto, A 2018, 'Assessment of metal concentrations in the SOD1G93A mouse model of amyotrophic lateral sclerosis and its potential role in muscular denervation, with particular focus on muscle tissue.', Molecular and cellular neurosciences, vol. 88, pp. 319-329.View/Download from: UTS OPUS or Publisher's site
BACKGROUND:Amyotrophic lateral sclerosis (ALS) is among the most common of the motor neuron diseases, and arguably the most devastating. During the course of this fatal neurodegenerative disorder, motor neurons undergo progressive degeneration. The currently best-understood animal models of ALS are based on the over-expression of mutant isoforms of Cu/Zn superoxide dismutase 1 (SOD1); these indicate that there is a perturbation in metal homeostasis with disease progression. Copper metabolism in particular is affected in the central nervous system (CNS) and muscle tissue. METHODS:This present study assessed previously published and newly gathered concentrations of transition metals (Cu, Zn, Fe and Se) in CNS (brain and spinal cord) and non-CNS (liver, intestine, heart and muscle) tissues from transgenic mice over-expressing the G93A mutant SOD1 isoform (SOD1G93A), transgenic mice over-expressing wildtype SOD1 (SOD1WT) and non-transgenic controls. RESULTS:Cu accumulates in non-CNS tissues at pre-symptomatic stages in SOD1G93A tissues. This accumulation represents a potentially pathological feature that cannot solely be explained by the over-expression of mSOD1. As a result of the lack of Cu uptake into the CNS there may be a deficiency of Cu for the over-expressed mutant SOD1 in these tissues. Elevated Cu concentrations in muscle tissue also preceded the onset of symptoms and were found to be pathological and not be the result of SOD1 over-expression. CONCLUSIONS:It is hypothesized that the observed Cu accumulations may represent a pathologic feature of ALS, which may actively contribute to axonal retraction leading to muscular denervation, and possibly significantly contributing to disease pathology. Therefore, it is proposed that the toxic-gain-of-function and dying-back hypotheses to explain the molecular drivers of ALS may not be separate, individual processes; rather our data suggests that they are parallel processes.
Gissi, F, Stauber, JL, Binet, MT, Trenfield, MA, Van Dam, JW & Jolley, DF 2018, 'Assessing the chronic toxicity of nickel to a tropical marine gastropod and two crustaceans.', Ecotoxicology and environmental safety, vol. 159, pp. 284-292.View/Download from: UTS OPUS or Publisher's site
The mining and processing of nickel ores from tropical regions contributes 40% of the global supply. The potential impact of these activities on tropical marine ecosystems is poorly understood. Due to the lack of ecotoxicity data for tropical marine species, there is currently no available water quality guideline value for nickel that is specific to tropical species. In this study, we investigated the toxicity of nickel to three tropical marine invertebrates, the gastropod Nassarius dorsatus, the barnacle Amphibalanus amphitrite, and the copepod Acartia sinjiensis. All toxicity tests used chronic endpoints, namely larval growth, metamorphosis (transition from nauplii to cyprid larvae) and larval development for the snail, barnacle and copepod respectively. Toxicity tests were carried out under environmentally relevant conditions (i.e. 27-30ᵒC, salinity 34-36‰, pH 8.1-8.4). Copper was also tested for quality assurance purposes and to allow for comparisons with previous studies. The copepod was the most sensitive species to nickel, with development inhibited by 10% (EC10) at 5.5 (5.0-6.0) µg Ni/L (95% confidence limits (CL)). Based on EC10 values, the gastropod and barnacle showed similar sensitivities to nickel with growth and metamorphosis inhibited by 10% at 64 (37-91) µg Ni/L and 67 (53-80) µg Ni/L, respectively. Based on existing data available in the literature, the copepod A. sinjiensis is so far the most sensitive tropical marine species to nickel. This study has provided high quality data which will contribute to the development of a water quality guideline value for nickel in tropical marine waters. A species sensitivity distribution of chronic nickel toxicity used the data generated in this paper supplemented by available literature data, comprising 12 species representing 6 taxonomic groups. A 5% hazard concentration (HC5) was determined as 8.2 µg/L Ni.
Holland, A, Stauber, J, Wood, CM, Trenfield, M & Jolley, DF 2018, 'Dissolved organic matter signatures vary between naturally acidic, circumneutral and groundwater-fed freshwaters in Australia.', Water research, vol. 137, pp. 184-192.View/Download from: UTS OPUS or Publisher's site
Dissolved organic matter (DOM) plays important roles in both abiotic and biotic processes within aquatic ecosystems, and these in turn depend on the quality of the DOM. We collected and characterized chromophoric DOM (CDOM) from different Australian freshwater types (circumneutral, naturally acidic and groundwater-fed waterways), climatic regions and seasons. CDOM quality was characterized using absorbance and fluorescence spectroscopy. Excitation emission scans followed by parallel factor (PARAFAC) analysis showed that CDOM was characterized by three main components: protein-like, fulvic-like and humic-like components commonly associated with various waters globally in the Openfluor database. Principal component analysis showed that CDOM quality varied between naturally acidic, circumneutral and groundwater-fed waters, with unique CDOM quality signatures shown for each freshwater type. CDOM quality also differed significantly within some sites between seasons. Clear differences in dominant CDOM components were shown between freshwater types. Naturally acidic waters were dominated by highly aromatic (as indicated by the specific absorbance co-efficient (SAC340) and the specific UV absorbance (SUVA254) values which ranged between 31 and 50 cm2 mg-1 and 3.9-5.7 mg C-1 m-1 respectively), humic-like CDOM of high molecular weight (as indicated by abs254/365 which ranged from 3.8 to 4.3). In contrast, circumneutral waters were dominated by fulvic-like CDOM of lower aromaticity (SAC340: 7-21 cm2 mg-1 and SUVA254: 1.5-3.0 mg C-1 m-1) and lower molecular weight (abs254/365 5.1-9.3). The groundwater-fed site had a higher abundance of protein-like CDOM, which was the least aromatic (SAC340: 2-5 cm2 mg-1 and SUVA254: 0.58-1.1 mg C-1 m-1). CDOM was generally less aromatic, of a lower molecular weight and more autochthonous in nature during the summer/autumn sampling compared to winter/spring. Significant relationships were shown between various CDOM quality parameters and pH....
Koppel, DJ, Adams, MS, King, CK & Jolley, DF 2018, 'Chronic toxicity of an environmentally relevant and equitoxic ratio of five metals to two Antarctic marine microalgae shows complex mixture interactivity.', Environmental pollution (Barking, Essex : 1987), vol. 242, no. Pt B, pp. 1319-1330.View/Download from: UTS OPUS or Publisher's site
Metal contaminants are rarely present in the environment individually, yet environmental quality guidelines are derived from single-metal toxicity data. Few metal mixture studies have investigated more than binary mixtures and many are at unrealistically high effect concentrations to freshwater organisms. This study investigates the toxicity of five metals (Cd, Cu, Ni, Pb, and Zn) to the Antarctic marine microalgae Phaeocystis antarctica and Cryothecomonas armigera. Two mixtures were tested: (i) an equitoxic mixture of contaminants present at their single-metal EC10 concentrations, and (ii) an environmental mixture based on the ratio metal concentrations in a contaminated Antarctic marine bay. Observed toxicity, as chronic population growth rate inhibition, was compared to Independent Action (IA) and Concentration Addition (CA) predictions parameterised to use EC10 values. This allowed for the inclusion of metals with low toxicities. The biomarkers chlorophyll a fluorescence, cell size and complexity, and intracellular lipid concentrations were assessed to investigate possible mechanisms behind metal-mixture interactions. Both microalgae had similar responses to the equitoxic mixture: non-interactive by IA and antagonistic by CA. Toxicity from the environmental mixture was antagonistic by IA to P. antarctica; however, to C. armigera it was concentration-dependent with antagonism at low toxicities and synergism at high toxicities by both IA and CA. Differences in dissolved organic carbon production and detoxification mechanisms may be responsible for these responses and warrants further investigation. This study shows that mixture toxicity interactions can be ratio, species, and concentration dependent. The responses of the microalgae to different mixture ratios highlight the need to assess toxicity at environmentally realistic metal ratios. Parameterising IA and CA reference models to use EC10s allowed for the inclusion of metals at low effect concentrations, whi...
Long, M, Tallec, K, Soudant, P, Lambert, C, Le Grand, F, Sarthou, G, Jolley, D & Hégaret, H 2018, 'A rapid quantitative fluorescence-based bioassay to study allelochemical interactions from Alexandrium minutum.', Environmental pollution (Barking, Essex : 1987), vol. 242, no. Pt B, pp. 1598-1605.View/Download from: UTS OPUS or Publisher's site
Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods. Allelochemical interactions are usually quantified through population growth inhibition or lytic-activity based bioassays using a secondary target organism. However these bioassays require time (for growth or microalgal counts) and/or are based on lethal effects. The use of pulse amplitude modulation (PAM) fluorometry has been widely used to assess the impact of environmental stressors on phytoplankton but rarely for allelochemical interactions. Here we evaluated the use of PAM and propose a rapid chlorophyll fluorescence based bioassay to quantify allelochemical BECs released from Alexandrium minutum. We used the ubiquitous diatom Chaetoceros muelleri as a target species. The bioassay, based on sub-lethal effects, quantifies allelochemical activity from different samples (filtrates, extracts in seawater) within a short period of time (2 h). This rapid bioassay will help investigate the role of allelochemical interactions in Alexandrium bloom establishment. It will also further our understanding of the potential relationship between allelochemical activities and other cytotoxic activities from BECs. While this bioassay was developed for the species A. minutum, it may be applicable to other species producing allelochemic...
Long, M, Tallec, K, Soudant, P, Le Grand, F, Donval, A, Lambert, C, Sarthou, G, Jolley, DF & Hégaret, H 2018, 'Allelochemicals from Alexandrium minutum induce rapid inhibition of metabolism and modify the membranes from Chaetoceros muelleri', Algal Research, vol. 35, pp. 508-518.View/Download from: UTS OPUS or Publisher's site
© 2018 Elsevier B.V. Allelochemical interactions are likely to be a contributing factor explaining the success of large blooms of the harmful marine dinoflagellate Alexandrium, however, the physiological mechanisms of allelochemical interactions remain poorly described. Here we investigated the sub-lethal effects (on an hourly scale) of a filtrate containing allelochemicals from Alexandrium minutum on the physiology of the common diatom Chaetoceros muelleri. The filtrate induced deleterious effects to the diatom physiology within only 30 min of exposure. Esterase activity and photosynthesis were drastically inhibited, with up to 34% of the population being metabolically inactive and up to 30% reduction in photosystem II quantum yield when exposed to the filtrate. In addition, intracellular reactive oxygen species increased by 26% in response to allelochemical exposure. C. muelleri pigment and lipid analyses indicated that the photosystem II was inhibited, with photoinhibition-like responses (activation of xanthophyll cycles, and changes in associated lipids) upregulated to mitigate the toxic effects of allelochemicals. Changes in the proportions of membrane lipid classes and increased membrane fatty acids saturation by 9% may be an attempt to maintain membrane integrity and associated enzyme activity, or could be the result of deleterious effects on membranes. An 8% decrease in cellular storage lipids (triglycerides) revealed a mobilization of energy suggesting an energetic cost for the diatom to counteract the allelochemical effects. We hypothesize that the rapid alteration of physiological functions such as photosynthesis and some enzymatic activities may result from direct damage on external membranes. Overall this study describes the sub-lethal mechanisms and provides useful biomarkers to understand the role of allelochemical interactions and associated ecological processes in structuring plankton communities.
Remaili, TM, Simpson, SL, Bennett, WW, King, JJ, Mosley, LM, Welsh, DT & Jolley, DF 2018, 'Assisted natural recovery of hypersaline sediments: salinity thresholds for the establishment of a community of bioturbating organisms.', Environmental science. Processes & impacts, vol. 20, no. 9, pp. 1244-1253.View/Download from: UTS OPUS or Publisher's site
Hypersaline sediments derived from poor land management or the decommissioning of large-scale salt production contribute to the long-term degradation of aquatic environments. Obstacles impeding remediation of these environments include salt crusts restricting benthic recolonisation, hypersalinity-induced toxicity to organisms, and disruption of biogeochemical cycles. Remediation often focuses on engineered solutions, despite sediment-biota interactions often playing a crucial role in improving long-term remediation and restoration of contaminated areas. The presence of extensive bioturbating communities can assist with flushing of excess salt ions, and the reduction of excess nutrients. Here we investigated the tolerance limits that may impede benthic organism recolonisation of hypersaline sediments. Bioassays on dilutions of a hypersaline sediment (∼400 psu (practical salinity units)) and extracted porewaters were used to assess the acute and chronic tolerances of a range of benthic species. Amphipod, copepod and shrimp species were the least tolerant to hypersalinity; bivalve and gastropod species displayed intermediate tolerance; and crab and polychaete species were the most tolerant, i.e. able to endure prolonged exposure in waters at ≥60 psu. Avoidance tests found many species avoid salinities >50 psu. Short-term endurance tests (time to death) indicated thresholds in the 52-70 psu range through tidal cycle exposures of 6 h (semi-diurnal), 12 h (diurnal), 24 h and 48 h (prolonged). Amphipod reproduction and shrimp larvae development bioassays had EC30's of 46 psu and EC50's in the 54-65 psu range, indicating potential to maintain populations at salinities up to 65 psu. These results will assist in designing successful monitored natural recovery strategies for salt ponds that may supplement the initial engineered approaches.
Remaili, TM, Yin, N, Bennett, WW, Simpson, SL, Jolley, DF & Welsh, DT 2018, 'Contrasting effects of bioturbation on metal toxicity of contaminated sediments results in misleading interpretation of the AVS-SEM metal-sulfide paradigm.', Environmental science. Processes & impacts, vol. 20, no. 9, pp. 1285-1296.View/Download from: UTS OPUS or Publisher's site
In undisturbed, metal-contaminated marine sediments, porewater metal concentrations are generally low due to their associations with strong binding phases such as organic matter, Fe/Mn (oxy)hydroxides and sulfides. Bioturbating fauna can alter redox conditions and, therefore, metal binding, potentially leading to increased metal bioavailability and subsequent toxicity to inhabiting organisms. Here we assessed the impacts of bioturbation (by bivalves and large amphipod species) on sediment biogeochemistry, metal bioaccumulation and toxicity to a smaller amphipod species in a metal contaminated sediment with low and high acid volatile sulfide (AVS) concentrations. Active bioturbation lowered metal toxicity to reproduction in the sediment with low-AVS (from 90% toxic (non-bioturbated) to 50% toxic (bioturbated)). This corresponded with lower dissolved metal concentrations in the overlying water column and lower metal bioaccumulation. Conversely, toxicity increased due to bioturbation in the sediment with high-AVS (40% toxic (non-bioturbated) to 80% toxic (bioturbated)), coinciding with sulfide oxidation, metal release and greater metal bioaccumulation. The results indicate that the AVS-SEM paradigm (commonly used to estimate the risks of adverse effects to benthic organisms in metal-contaminated sediments) may result in incorrect assessment outcomes in cases where bioturbating organisms rework and oxidize the sediment, or for those sediments where AVS has accumulated due to the inability of larger bioturbating benthic organisms to establish populations.
Angel, BM, Simpson, SL, Granger, E, Goodwyn, K & Jolley, DF 2017, 'Time-averaged concentrations are effective for predicting chronic toxicity of varying copper pulse exposures for two freshwater green algae species', ENVIRONMENTAL POLLUTION, vol. 230, pp. 787-797.View/Download from: Publisher's site
Enge, TG, Ecroyd, H, Jolley, DF, Yerbury, JJ & Dosseto, A 2017, 'Longitudinal assessment of metal concentrations and copper isotope ratios in the G93A SOD1 mouse model of amyotrophic lateral sclerosis', METALLOMICS, vol. 9, no. 2, pp. 161-174.View/Download from: Publisher's site
Gissi, F, Stauber, J, Reichelt-Brushett, A, Harrison, PL & Jolley, DF 2017, 'Inhibition in fertilisation of coral gametes following exposure to nickel and copper', ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, vol. 145, pp. 32-41.View/Download from: Publisher's site
Koppel, DJ, Gissi, F, Adams, MS, King, CK & Jolley, DF 2017, 'Chronic toxicity of five metals to the polar marine microalga Cryothecomonas armigera - Application of a new bioassay', ENVIRONMENTAL POLLUTION, vol. 228, pp. 211-221.View/Download from: Publisher's site
Mason, TJ, French, K & Jolley, DF 2017, 'Functional Richness and Identity Do Not Strongly Affect Invasibility of Constructed Dune Communities', PLOS ONE, vol. 12, no. 1.View/Download from: UTS OPUS or Publisher's site
Remaili, TM, Simpson, SL & Jolley, DF 2017, 'Effects of enhanced bioturbation intensities on the toxicity assessment of legacy-contaminated sediments', ENVIRONMENTAL POLLUTION, vol. 226, pp. 335-345.View/Download from: Publisher's site
Adams, MS, Dillon, CT, Vogt, S, Lai, B, Stauber, J & Jolley, DF 2016, 'Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 50, no. 16, pp. 8827-8839.View/Download from: Publisher's site
Amato, ED, Simpson, SL, Remaili, TM, Spadaro, DA, Jarolimek, CV & Jolley, DF 2016, 'Assessing the Effects of Bioturbation on Metal Bioavailability in Contaminated Sediments by Diffusive Gradients in Thin Films (DGT)', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 50, no. 6, pp. 3055-3064.View/Download from: Publisher's site
Enge, TG, Field, MP, Jolley, DF, Ecroyd, H, Kim, MH & Dosseto, A 2016, 'An automated chromatography procedure optimized for analysis of stable Cu isotopes from biological materials', JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, vol. 31, no. 10, pp. 2023-2030.View/Download from: Publisher's site
Gillmore, ML, Golding, LA, Angel, BM, Adams, MS & Jolley, DF 2016, 'Toxicity of dissolved and precipitated aluminium to marine diatoms', AQUATIC TOXICOLOGY, vol. 174, pp. 82-91.View/Download from: Publisher's site
Gissi, F, Stauber, JL, Binet, MT, Golding, LA, Adams, MS, Schlekat, CE, Garman, ER & Jolley, DF 2016, 'A review of nickel toxicity to marine and estuarine tropical biota with particular reference to the South East Asian and Melanesian region', ENVIRONMENTAL POLLUTION, vol. 218, pp. 1308-1323.View/Download from: Publisher's site
Jolley, DF, Wilson, SR, Kelso, C, O'Brien, G & Mason, CE 2016, 'Analytical Thinking, Analytical Action: Using Prelab Video Demonstrations and e-Quizzes To Improve Undergraduate Preparedness for Analytical Chemistry Practical Classes', JOURNAL OF CHEMICAL EDUCATION, vol. 93, no. 11, pp. 1855-1862.View/Download from: Publisher's site
Ngo, LK, Pinch, BM, Bennett, WW, Teasdale, PR & Jolley, DF 2016, 'Assessing the uptake of arsenic and antimony from contaminated soil by radish (Raphanus sativus) using DGT and selective extractions', ENVIRONMENTAL POLLUTION, vol. 216, pp. 104-114.View/Download from: Publisher's site
Remaili, TM, Simpson, SL, Amato, ED, Spadaro, DA, Jarolimek, CV & Jolley, DF 2016, 'The impact of sediment bioturbation by secondary organisms on metal bioavailability, bioaccumulation and toxicity to target organisms in benthic bioassays: Implications for sediment quality assessment', ENVIRONMENTAL POLLUTION, vol. 208, pp. 590-599.View/Download from: Publisher's site
Amato, ED, Simpson, SL, Belzunce-Segarra, MJ, Jarolimek, CV & Jolley, DF 2015, 'Metal Fluxes from Porewaters and Labile Sediment Phases for Predicting Metal Exposure and Bioaccumulation in Benthic Invertebrates', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 49, no. 24, pp. 14204-14212.View/Download from: Publisher's site
Angel, BM, Simpson, SL, Chariton, AA, Stauber, JL & Jolley, DF 2015, 'Time-averaged copper concentrations from continuous exposures predicts pulsed exposure toxicity to the marine diatom, Phaeodactylum tricornutum: Importance of uptake and elimination', AQUATIC TOXICOLOGY, vol. 164, pp. 1-9.View/Download from: Publisher's site
Belzunce-Segarra, MJ, Simpson, SL, Amato, ED, Spadaro, DA, Hamilton, IL, Jarolimek, CV & Jolley, DF 2015, 'The mismatch between bioaccumulation in field and laboratory environments: Interpreting the differences for metals in benthic bivalves', ENVIRONMENTAL POLLUTION, vol. 204, pp. 48-57.View/Download from: Publisher's site
Gissi, F, Adams, MS, King, CK & Jolley, DF 2015, 'A robust bioassay to assess the toxicity of metals to the antarctic marine microalga Phaeocystis antarctica', ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 34, no. 7, pp. 1578-1587.View/Download from: Publisher's site
Khaksar, M, Jolley, DF, Sekine, R, Vasilev, K, Johannessen, B, Donner, E & Lombi, E 2015, 'In Situ Chemical Transformations of Silver Nanoparticles along the Water-Sediment Continuum', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 49, no. 1, pp. 318-325.View/Download from: Publisher's site
Macoustra, GK, King, CK, Wasley, J, Robinson, SA & Jolley, DF 2015, 'Impact of hydrocarbons from a diesel fuel on the germination and early growth of subantarctic plants', ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS, vol. 17, no. 7, pp. 1238-1248.View/Download from: Publisher's site
Nydahl, AC, King, CK, Wasley, J, Jolley, DF & Robinson, SA 2015, 'Toxicity of fuel-contaminated soil to Antarctic moss and terrestrial algae', ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 34, no. 9, pp. 2004-2012.View/Download from: Publisher's site
Amato, ED, Simpson, SL, Jarolimek, CV & Jolley, DF 2014, 'Diffusive Gradients in Thin Films Technique Provide Robust Prediction of Metal Bioavailability and Toxicity in Estuarine Sediments', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 48, no. 8, pp. 4485-4494.View/Download from: Publisher's site
Lopez, LK, Couture, P, Maher, WA, Krikowa, F, Jolley, DF & Davis, AR 2014, 'Response of the hairy mussel Trichomya hirsuta to sediment-metal contamination in the presence of a bioturbator', MARINE POLLUTION BULLETIN, vol. 88, no. 1-2, pp. 180-187.View/Download from: Publisher's site
Simpson, SL, Vardanega, CR, Jarolimek, C, Jolley, DF, Angel, BM & Mosley, LM 2014, 'Metal speciation and potential bioavailability changes during discharge and neutralisation of acidic drainage water', CHEMOSPHERE, vol. 103, pp. 172-180.View/Download from: Publisher's site
Smith, CL, Stauber, JL, Wilson, MR & Jolley, DF 2014, 'The use of immobilised metal affinity chromatography (IMAC) to compare expression of copper-binding proteins in control and copper-exposed marine microalgae', ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 406, no. 1, pp. 305-315.View/Download from: Publisher's site
Smith, CL, Steele, JE, Stauber, JL & Jolley, DF 2014, 'Copper-induced changes in intracellular thiols in two marine diatoms: Phaeodactylum tricornutum and Ceratoneis closterium', AQUATIC TOXICOLOGY, vol. 156, pp. 211-220.View/Download from: Publisher's site
Mason, TJ, French, K & Jolley, D 2013, 'Arrival order among native plant functional groups does not affect invasibility of constructed dune communities', OECOLOGIA, vol. 173, no. 2, pp. 557-568.View/Download from: Publisher's site
Price, HL, Teasdale, PR & Jolley, DF 2013, 'An evaluation of ferrihydrite- and Metsorb (TM)-DGT techniques for measuring oxyanion species (As, Se, V, P): Effective capacity, competition and diffusion coefficients', ANALYTICA CHIMICA ACTA, vol. 803, pp. 56-65.View/Download from: Publisher's site
Ward, DJ, Simpson, SL & Jolley, DF 2013, 'Avoidance of contaminated sediments by an amphipod (Melita plumulosa), A harpacticoid copepod (Nitocra spinipes), and a snail (Phallomedusa solida)', ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 32, no. 3, pp. 644-652.View/Download from: Publisher's site
Ward, DJ, Simpson, SL & Jolley, DF 2013, 'Slow Avoidance Response to Contaminated Sediments Elicits Sublethal Toxicity to Benthic Invertebrates', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 47, no. 11, pp. 5947-5953.View/Download from: Publisher's site
Bennett, WW, Teasdale, PR, Panther, JG, Welsh, DT, Zhao, H & Jolley, DF 2012, 'Investigating Arsenic Speciation and Mobilization in Sediments with DGT and DET: A Mesocosm Evaluation of Oxic-Anoxic Transitions', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 46, no. 7, pp. 3981-3989.View/Download from: Publisher's site
Bennett, WW, Teasdale, PR, Welsh, DT, Panther, JG & Jolley, DF 2012, 'Optimization of colorimetric DET technique for the in situ, two-dimensional measurement of iron(II) distributions in sediment porewaters', TALANTA, vol. 88, pp. 490-495.View/Download from: Publisher's site
Bennett, WW, Teasdale, PR, Welsh, DT, Panther, JG, Stewart, RR, Price, HL & Jolley, DF 2012, 'Inorganic arsenic and iron(II) distributions in sediment porewaters investigated by a combined DGT-colourimetric DET technique', ENVIRONMENTAL CHEMISTRY, vol. 9, no. 1, pp. 31-40.View/Download from: Publisher's site
Lelong, A, Jolley, DF, Soudant, P & Hegaret, H 2012, 'Impact of copper exposure on Pseudo-nitzschia spp. physiology and domoic acid production', AQUATIC TOXICOLOGY, vol. 118, pp. 37-47.View/Download from: Publisher's site
Simpson, SL, Ward, D, Strom, D & Jolley, DF 2012, 'Oxidation of acid-volatile sulfide in surface sediments increases the release and toxicity of copper to the benthic amphipod Melita plumulosa', CHEMOSPHERE, vol. 88, no. 8, pp. 953-961.View/Download from: Publisher's site
Simpson, SL, Yverneau, H, Cremazy, A, Jarolimek, CV, Price, HL & Jolley, DF 2012, 'DGT-Induced Copper Flux Predicts Bioaccumulation and Toxicity to Bivalves in Sediments with Varying Properties', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 46, no. 16, pp. 9038-9046.View/Download from: Publisher's site
Bennett, WW, Teasdale, PR, Panther, JG, Welsh, DT & Jolley, DF 2011, 'Speciation of Dissolved Inorganic Arsenic by Diffusive Gradients in Thin Films: Selective Binding of As-III by 3-Mercaptopropyl-Functionalized Silica Gel', ANALYTICAL CHEMISTRY, vol. 83, no. 21, pp. 8293-8299.View/Download from: Publisher's site
Levy, JL, Stauber, JL, Wakelin, SA & Jolley, DF 2011, 'The effect of field-collected biofilms on the toxicity of copper to a marine microalga (Tetraselmis sp.) in laboratory bioassays', MARINE AND FRESHWATER RESEARCH, vol. 62, no. 12, pp. 1362-1372.View/Download from: Publisher's site
Strom, D, Simpson, SL, Batley, GE & Jolley, DF 2011, 'THE INFLUENCE OF SEDIMENT PARTICLE SIZE AND ORGANIC CARBON ON TOXICITY OF COPPER TO BENTHIC INVERTEBRATES IN OXIC/SUBOXIC SURFACE SEDIMENTS', ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 30, no. 7, pp. 1599-1610.View/Download from: Publisher's site
Ward, DJ, Perez-Landa, V, Spadaro, DA, Simpson, SL & Jolley, DF 2011, 'An Assessment of Three Harpacticoid Copepod Species for Use in Ecotoxicological Testing', ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, vol. 61, no. 3, pp. 414-425.View/Download from: Publisher's site
Angel, BM, Hales, LT, Simpson, SL, Apte, SC, Chariton, AA, Shearer, DA & Jolley, DF 2010, 'Spatial variability of cadmium, copper, manganese, nickel and zinc in the Port Curtis Estuary, Queensland, Australia', MARINE AND FRESHWATER RESEARCH, vol. 61, no. 2, pp. 170-183.View/Download from: Publisher's site
Angel, BM, Simpson, SL & Jolley, DF 2010, 'TOXICITY TO MELITA PLUMULOSA FROM INTERMITTENT AND CONTINUOUS EXPOSURES TO DISSOLVED COPPER', ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 29, no. 12, pp. 2823-2830.View/Download from: Publisher's site
Bennett, WW, Teasdale, PR, Panther, JG, Welsh, DT & Jolley, DF 2010, 'New Diffusive Gradients in a Thin Film Technique for Measuring Inorganic Arsenic and Selenium(IV) Using a Titanium Dioxide Based Adsorbent', ANALYTICAL CHEMISTRY, vol. 82, no. 17, pp. 7401-7407.View/Download from: Publisher's site
Levy, JL, Stauber, JL, Wakelin, SA & Jolley, DF 2009, 'The effect of bacteria on the sensitivity of microalgae to copper in laboratory bioassays', CHEMOSPHERE, vol. 74, no. 9, pp. 1266-1274.View/Download from: Publisher's site
McKinnon, J, Gribben, PE, Davis, A, Jolley, DF & Wright, JT 2009, 'Differences in soft-sediment macrobenthic assemblages invaded by Caulerpa taxifolia compared to uninvaded habitats', Marine Ecology Progress Series, vol. 380, no. 1, pp. 59-71.View/Download from: UTS OPUS or Publisher's site
Caulerpa taxifolia is a habitat-forming green alga that has invaded several temperateregions worldwide. Although C. taxifolia covers large areas of soft-sediment habitat, little is knownabout its effects on soft-sediment invertebrate assemblages. We compared soft-sediment macroinvertebrateassemblages in 2 estuaries in southeastern Australia invaded by C. taxifolia to examine 2main predictions: (1) areas covered with C. taxifolia will have different assemblages compared tounvegetated sediment because infauna are inhibited but epifauna are facilitated, and (2) areas withC. taxifolia will have different assemblages compared to those with native seagrasses (Halophilaovalis and Zostera capricorni) because infauna are inhibited but epifauna are not. Multidimensionalscaling and ANOSIM showed differences in invertebrate assemblages between all habitats. In C. taxifolia,infauna were less abundant and epifauna were more abundant compared to unvegetated sediment.However, when compared to native seagrasses, epifauna in C. taxifolia were more abundantthan in H. ovalis in one estuary but less abundant than in Z. capricorni in another estuary, whileinfauna in C. taxifolia were less abundant than in both seagrass species. The consistently low infaunalabundance in C. taxifolia, irrespective of infaunal feeding mode, suggests C. taxifolia impactsinfauna generally. Examination of environmental factors potentially responsible for the low abundanceof infauna indicated that differences in redox potential (and associated chemical changes) mayexplain patterns in abundance of infauna among habitats. Our findings indicate that invasion by C.taxifolia causes important changes to soft-sediment macroinvertebrate assemblages and suggest thatinfauna may be particularly vulnerable to invasion because of changes to sediment chemistry.
Melville, F, Andersen, LE & Jolley, DF 2009, 'The Gladstone (Australia) oil spill - Impacts on intertidal areas: Baseline and six months post-spill', MARINE POLLUTION BULLETIN, vol. 58, no. 2, pp. 263-271.View/Download from: Publisher's site
Andersen, LE, Melville, F & Jolley, D 2008, 'An assessment of an oil spill in Gladstone, Australia - Impacts on intertidal areas at one month post-spill', MARINE POLLUTION BULLETIN, vol. 57, no. 6-12, pp. 607-615.View/Download from: Publisher's site
Levy, JL, Angel, BM, Stauber, JL, Poon, WL, Simpson, SL, Cheng, SH & Jolley, DF 2008, 'Uptake and internalisation of copper by three marine microalgae: Comparison of copper-sensitive and copper-tolerant species', AQUATIC TOXICOLOGY, vol. 89, no. 2, pp. 82-93.View/Download from: Publisher's site
Threlfall, CG, Jolley, DF, Evershed, N, Goldingay, RL & Buttemer, WA 2008, 'Do Green and Golden Bell Frogs Litoria aurea occupy habitats with fungicidal properties?', Australian Zoologist, vol. 34, no. 3, pp. 350-360.View/Download from: Publisher's site
The Green and Golden Bell frog Litoria aurea is in major decline in Australia, where its distribution is now confined mainly to the east coast of New South Wales (NSW). Infection by the newly emerged amphibian fungal pathogen Batrachochytrium dendrobatidis has been identified as one of the main threats affecting L aurea. Surprisingly, some of the sites in NSW sustaining the largest populations of this species are industrial and urban habitats that are often disturbed and polluted, which could protect L aurea from chytrid infection if pollution had fungicidal capacity. The aim of this study was to characterise the trace metal concentration of several L aurea breeding sites in the Sydney and Illawarra. regions of NSW and to evaluate the fungicidal efficacy of the main trace metals identified. Selected L aurea sites were sampled throughout the breeding season (September to February) to establish the concentration of trace metals in both surface sediment and waters. Physico-chemical parameters including pH and salinity were also measured. Of the trace metals identified, copper and zinc were consistently elevated across sites. Over 50% of sites exceeded the National Sediment Quality Guideline for both copper and zinc concentration, and over 90% of sites exceeded the National Water Quality Guideline for these metals. Consequently, we evaluated their effect on the growth and survival of a laboratory culture of B. dendrobatidis. These tests were performed in media containing dissolved metal concentrations of 0.02 - 0.65 mgL-1 Cu and 0.24 - 5.0 mgL-1 Zn. Growth rates were inferred by total fungal density in liquid culture (based on spectral absorbance measurements), final dry weight, and the density of zoospores in fungal cultures grown for 28 days. Both copper and zinc were found to reduce the growth and proliferation of B. dendrobatidis, but in a non-linear manner.This suggests that L aurea may be gaining some protection from B. dendrobatidis infection at several of the...
Atkinson, CA, Jolley, DF & Simpson, SL 2007, 'Effect of overlying water pH, dissolved oxygen, salinity and sediment disturbances on metal release and sequestration from metal contaminated marine sediments', CHEMOSPHERE, vol. 69, no. 9, pp. 1428-1437.View/Download from: Publisher's site
Johnson, HL, Stauber, JL, Adams, MS & Jolley, DF 2007, 'Copper and zinc tolerance of two tropical microalgae after copper acclimation', ENVIRONMENTAL TOXICOLOGY, vol. 22, no. 3, pp. 234-244.View/Download from: Publisher's site
Levy, JL, Stauber, JL & Jolley, DF 2007, 'Sensitivity of marine microalgae to copper: The effect of biotic factors on copper adsorption and toxicity', SCIENCE OF THE TOTAL ENVIRONMENT, vol. 387, no. 1-3, pp. 141-154.View/Download from: Publisher's site
Parkes, SD, Jolley, DF & Wilson, SR 2007, 'Inorganic nitrogen transformations in the treatment of landfill leachate with a high ammonium load: A case study', ENVIRONMENTAL MONITORING AND ASSESSMENT, vol. 124, no. 1-3, pp. 51-61.View/Download from: Publisher's site
Simpson, SL, Burston, VL, Jolley, DF & Chau, K 2006, 'Application of surrogate methods for assessing the bioavailability of PAHs in sediments to a sediment ingesting bivalve', CHEMOSPHERE, vol. 65, no. 11, pp. 2401-2410.View/Download from: Publisher's site
Levy, JL, Stauber, JL, Adams, MS, Maher, WA, Kirby, JK & Jolley, DF 2005, 'Toxicity, biotransformation, and mode of action of arsenic in two freshwater microalgae (Chlorella sp and Monoraphidium arcuatum)', ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 24, no. 10, pp. 2630-2639.View/Download from: Publisher's site
King, CK, Dowse, MC, Simpson, SL & Jolley, DF 2004, 'An assessment of five Australian polychaetes and bivalves for use in whole-sediment toxicity tests: Toxicity and accumulation of copper and zinc from water and sediment', ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, vol. 47, no. 3, pp. 314-323.View/Download from: Publisher's site
Simpson, SL, Angel, BM & Jolley, DF 2004, 'Metal equilibration in laboratory-contaminated (spiked) sediments used for the development of whole-sediment toxicity tests', CHEMOSPHERE, vol. 54, no. 5, pp. 597-609.View/Download from: Publisher's site
Simpson, SL, Maher, EJ & Jolley, DF 2004, 'Processes controlling metal transport and retention as metal-contaminated groundwaters efflux through estuarine sediments', CHEMOSPHERE, vol. 56, no. 9, pp. 821-831.View/Download from: Publisher's site
Simpson, SL, Pryor, ID, Mewburn, BR, Batley, GE & Jolley, D 2002, 'Considerations for capping metal-contaminated sediments in dynamic estuarine environments', Environmental Science and Technology, vol. 36, no. 17, pp. 3772-3778.View/Download from: Publisher's site
The effects of tides, bioturbating organisms, and periods of anoxia on metal fluxes from contaminated harbor sediments in a shallow tidal estuarine bay were studied, together with capping technology options for the containment of metal contaminants. Zinc fluxes from the sediments were high, ranging from 10 to 89 mg of Zn m-2 day-1. In the absence of capping, experiments in corer-reactors showed that simulated tidal processes increased zinc fluxes 5-fold. Fluxes were also greater in the presence of sediment-dwelling organisms. If organisms were removed, and recolonizing organisms later added, their bioturbation activities initially lowered zinc fluxes, but fluxes gradually reached steady state at the higher levels seen previously. Capping materials physically isolate contaminated sediments, provide a binding substrate for metals released from the sediment, and importantly create an anoxic environment below the cap, which stimulates the formation of insoluble metal sulfides. Clean sediment (5 mm) was the most effective capping material in reducing zinc fluxes. Zeolite/sand mixtures (10 mm) also greatly reduced these fluxes, but significant breakthrough of zinc occurred after 2 weeks. Sand (20 mm) was not effective. The presence of organisms disturbed capping materials and increased zinc fluxes. Installed capping materials should have depths of >30 cm to minimize organisms burrowing to contaminated sediments beneath.
Simpson, SL, Pryor, ID, Mewburn, BR, Batley, GE & Jolley, D 2002, 'Considerations for capping metal-contaminated sediments in dynamic estuarine environments', ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 36, no. 17, pp. 3772-3778.View/Download from: Publisher's site
Jolley, D, Maher, W & Cullen, P 1998, 'Rapid method for separating and quantifying orthophosphate and polyphosphates: Application to sewage samples', WATER RESEARCH, vol. 32, no. 3, pp. 711-716.View/Download from: Publisher's site
Maher, W, Deaker, M, Jolley, D, Krikowa, F & Roberts, B 1997, 'Selenium occurrence, distribution and speciation in the cockle Anadara trapezia and the mullet Mugil cephalus', APPLIED ORGANOMETALLIC CHEMISTRY, vol. 11, no. 4, pp. 313-326.
Bennett, WW, Teasdale, PR, Panther, JG, Welsh, DT, Zhao, H & Jolley, DF 2012, 'Arsenic mobilization in sediments: An investigation using in situ sampling techniques', UNDERSTANDING THE GEOLOGICAL AND MEDICAL INTERFACE OF ARSENIC, AS 2012, 4th International Congress on Arsenic in the Environment - Understanding the Geological and Medical Interface (As), CRC PRESS-TAYLOR & FRANCIS GROUP, Cairns, AUSTRALIA, pp. 367-369.
Lelong, A, Jolley, DF, Hegaret, H, Kraffe, E & Soudant, P 2011, 'THE EFFECTS OF COPPER TOXICITY ON PSEUDO-NITZSCHIA SPP. PHYSIOLOGY AND DOMOIC ACID PRODUCTION', JOURNAL OF PHYCOLOGY, WILEY-BLACKWELL, pp. S25-S25.
Peters, GM, Maher, WA, Jolley, D, Carroll, BI, Gomes, VG, Jenkinson, AV & McOrist, GD 1998, 'Selenium contamination, redistribution and remobilisation in sediments of Lake Macquarie, NSW', ORGANIC GEOCHEMISTRY, Australian Organic Geochemistry Conference, PERGAMON-ELSEVIER SCIENCE LTD, CANBERRA, AUSTRALIA, pp. 1287-1300.View/Download from: Publisher's site