I'm a marine biologist with research interests in photophysiology,and productivity of aquatic primary producers, ranging from microalgae to corals. Prior to joining UTS as an ARC Future Fellow I was a Postdoctoral Research Fellow, and then lecturer in Marine Biogeochemistry, at the University of Essex, UK.
At UTS I am a core member of The Climate Change Cluster (C3) where my research focuses on improving marine primary productivity estimates using advanced active fluorometry (bio-optical) approaches. The outcomes from this research will provide more accurate information on the health of Australian coastal waters and be used to improve Global Climate Models.
I am also the leader for the C3 Future Reefs research program where our diverse research team examines how changing environments influence the capacity of corals to grow and survive. The outcomes of this work are being used to inform future reef trajectories, but also to develop new tools and decision-making towards improved stewardship of reef resources
- Subject Editor (Marine) for Global Change Biology (2018 IF = 8.88) since 2006.
- Associate Editor for Limnology & Oceanography: Methods (2018 IF = 2.09) since 2016.
- Lead coordinator, Great Barrier Reef Coral Nurture Program (https://www.coralnurtureprogram.org), 2019-2023.
- Steering Committee member, international Coral Restoration Consortium (http://crc.reefresilience.org), 2019-2023.
- Lead for international working group 156 on “Active Chlorophyll fluorescence for autonomous measurements of global marine primary productivity” (https://scor-int.org/group/156/), Scientific Committee on Ocean Research, 2019-2023.
- Invited member, IMOS Bio-Optical Working Group, 2015-2016.
- Invited contributor, Reef Future Genomics (ReFuGe) 2020 consortia, 2015-2016.
- International working group, Coral Bleaching Forecast Modeling 2012–2015.
- Co-founder/selection committee member, UK’s Coral Aquarists Research Network, 2011-2014.?
- Steering Committee member, UK Ocean Acidification program (Pelagic), 2010–2013.
- Assistant Director, UK’s Coral Reef Research Unit, 2005–2013.
- Member, European Network of Excellence for Ocean Ecosystems Analysis (EUROCEANS), 2007-2012.
- Convener/Chair/Organizer for ‘AQUAFLUO II: Fluorescence in Aquatic systems’ international conference (Sydney, 2017).
- Convener/Chair/Organizer for ‘AQUAFLUO: Fluorescence in Aquatic systems’ international conference (Czech Republic, 2007).
- Tomorrow Maker Awardee, AMP Foundation, 2018
- Outstanding contribution to UK Marine Science by a Young Scientist. Challenger Society UK, 2010
Can supervise: YES
Microalgal photophysiology and productivity in aquatic environments: Coupling of light harvesting, electron turnover and carbon fixation in key marine microalgae; electron sinks under light-nutrient perturbations; Mechanisms of stress induction (reactive oxygen production) and protection;
Photosystem biophysics; Fluorescence as a tool in aquatic sciences: Use of variable fluorescence to quantify microalgal primary production; Mechanistic models of photosynthesis and photochemistry;
Physiology & ecology of coral reef organisms: Physiological and molecular (omic) properties of zooxanthellae (Symbiodinium spp.) genetic variants; Coral adaptation and acclimation to marginal reef environments; Environmental control of coral growth and resource allocation;
Impacts of climate change on marine productivity: Influence of ocean acidification on growth and resource allocation of phytoplankton and corals; thermal stress impacts (mechanisms of coral bleaching) upon corals; changing light environments and photophysiology/primary productivity;
Algal secondary metabolite production: Regulation of isoprene production by phototrophs; interaction of DMS and oxidative stress of corals.
I am Subject Co-ordinator for Coral Reef Ecosystems 91126
Suggett, DJ, Borowitzka, M & Prášil, O 2010, Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications, Springer Science & Business Media.
This book follows on from the first international conference on "chlorophyll fluorescence in the aquatic sciences" (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through ...
Boström-Einarsson, L, Babcock, RC, Bayraktarov, E, Ceccarelli, D, Cook, N, Ferse, SCA, Hancock, B, Harrison, P, Hein, M, Shaver, E, Smith, A, Suggett, D, Stewart-Sinclair, PJ, Vardi, T & McLeod, IM 2020, 'Coral restoration – A systematic review of current methods, successes, failures and future directions', PLoS ONE, vol. 15, no. 1.View/Download from: Publisher's site
This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Coral reef ecosystems have suffered an unprecedented loss of habitat-forming hard corals in recent decades. While marine conservation has historically focused on passive habitat protection, demand for and interest in active restoration has been growing in recent decades. However, a disconnect between coral restoration practitioners, coral reef managers and scientists has resulted in a disjointed field where it is difficult to gain an overview of existing knowledge. To address this, we aimed to synthesise the available knowledge in a comprehensive global review of coral restoration methods, incorporating data from the peer-reviewed scientific literature, complemented with grey literature and through a survey of coral restoration practitioners. We found that coral restoration case studies are dominated by short-term projects, with 60% of all projects reporting less than 18 months of monitoring of the restored sites. Similarly, most projects are relatively small in spatial scale, with a median size of restored area of 100 m2. A diverse range of species are represented in the dataset, with 229 different species from 72 coral genera. Overall, coral restoration projects focused primarily on fast-growing branching corals (59% of studies), and report survival between 60 and 70%. To date, the relatively young field of coral restoration has been plagued by similar 'growing pains' as ecological restoration in other ecosystems. These include 1) a lack of clear and achievable objectives, 2) a lack of appropriate and standardised monitoring and reporting and, 3) poorly designed projects in relation to stated objectives. Mitigating these will be crucial to successfully scale up projects, and to retain public trust...
Clifton, J, Osman, EO, Suggett, DJ & Smith, DJ 2020, 'Resolving conservation and development tensions in a small island state: A governance analysis of Curieuse Marine National Park, Seychelles', Marine Policy, pp. 103650-103650.View/Download from: Publisher's site
Clifton, J, Osman, EO, Suggett, DJ & Smith, DJ 2020, 'Resolving conservation and development tensions in a small island state: A governance analysis of Curieuse Marine National Park, Seychelles', Marine Policy.View/Download from: Publisher's site
© 2019 Elsevier Ltd The management and conservation of marine resources in Seychelles, a small island developing state (SIDS) in the western Indian Ocean, is fundamental to maintaining the flow of international visitors which forms the mainstay of the nation's economy. There is an increasing trend towards empowering non-governmental organisations and parastatal entities with protected area management responsibilities, which partly reflects the chronic underfunding of the state protected area management institution. This paper explores these and related issues through a governance analysis of Curieuse Marine National Park, which is the most popular state-owned marine national park in terms of recorded visitor numbers. This demonstrates that the inability to implement economic incentives through not fully capitalising on the use and non-use values of the park has deleterious consequences for managing the combined impacts of tourism and fisheries on the ecological assets of the park. Furthermore, the capacity of the state management institution is being eroded through a focus on the development of an extensive network of new marine protected areas under the direction of an international non-governmental organisation. Suggestions are made that could strengthen economic, participative and interpretative incentives to provide a more sustainable basis for marine national park management.
Osman, EO, Suggett, DJ, Voolstra, CR, Pettay, DT, Clark, DR, Pogoreutz, C, Sampayo, EM, Warner, ME & Smith, DJ 2020, 'Coral microbiome composition along the northern Red Sea suggests high plasticity of bacterial and specificity of endosymbiotic dinoflagellate communities', Microbiome, vol. 8, no. 1.View/Download from: Publisher's site
© 2020 The Author(s). Background: The capacity of reef-building corals to tolerate (or adapt to) heat stress is a key factor determining their resilience to future climate change. Changes in coral microbiome composition (particularly for microalgal endosymbionts and bacteria) is a potential mechanism that may assist corals to thrive in warm waters. The northern Red Sea experiences extreme temperatures anomalies, yet corals in this area rarely bleach suggesting possible refugia to climate change. However, the coral microbiome composition, and how it relates to the capacity to thrive in warm waters in this region, is entirely unknown. Results: We investigated microbiomes for six coral species (Porites nodifera, Favia favus, Pocillopora damicornis, Seriatopora hystrix, Xenia umbellata, and Sarcophyton trocheliophorum) from five sites in the northern Red Sea spanning 4° of latitude and summer mean temperature ranges from 26.6 °C to 29.3 °C. A total of 19 distinct dinoflagellate endosymbionts were identified as belonging to three genera in the family Symbiodiniaceae (Symbiodinium, Cladocopium, and Durusdinium). Of these, 86% belonged to the genus Cladocopium, with notably five novel types (19%). The endosymbiont community showed a high degree of host-specificity despite the latitudinal gradient. In contrast, the diversity and composition of bacterial communities of the surface mucus layer (SML) - a compartment particularly sensitive to environmental change - varied significantly between sites, however for any given coral was species-specific. Conclusion: The conserved endosymbiotic community suggests high physiological plasticity to support holobiont productivity across the different latitudinal regimes. Further, the presence of five novel algal endosymbionts suggests selection of certain genotypes (or genetic adaptation) within the semi-isolated Red Sea. In contrast, the dynamic composition of bacteria associated with the SML across sites may contribute to holobiont ...
Osman, EO, Suggett, DJ, Voolstra, CR, Pettay, DT, Clark, DR, Pogoreutz, C, Sampayo, EM, Warner, ME & Smith, DJ 2020, 'Correction to: Coral microbiome composition along the northern Red Sea suggests high plasticity of bacterial and specificity of endosymbiotic dinoflagellate communities (Microbiome (2020) 8:8 DOI: 10.1186/s40168-019-0776-5)', Microbiome, vol. 8, no. 1.View/Download from: Publisher's site
© 2020 The Author(s). Reference. Following publication of the original article , the authors reported an error on the legend of of P.damicornis in Fig. 1.
Raven, JA, Suggett, DJ & Giordano, M 2020, 'Inorganic carbon concentrating mechanisms in free-living and symbiotic dinoflagellates and chromerids', Journal of Phycology.View/Download from: Publisher's site
© 2020 Phycological Society of America Photosynthetic dinoflagellates are ecologically and biogeochemically important in marine and freshwater environments. However, surprisingly little is known of how this group acquires inorganic carbon or how these diverse processes evolved. Consequently, how CO2 availability ultimately influences the success of dinoflagellates over space and time remains poorly resolved compared to other microalgal groups. Here we review the evidence. Photosynthetic core dinoflagellates have a Form II RuBisCO (replaced by Form IB or Form ID in derived dinoflagellates). The in vitro kinetics of the Form II RuBisCO from dinoflagellates are largely unknown, but dinoflagellates with Form II (and other) RuBisCOs have inorganic carbon concentrating mechanisms (CCMs), as indicated by in vivo internal inorganic C accumulation and affinity for external inorganic C. However, the location of the membrane(s) at which the essential active transport component(s) of the CCM occur(s) is (are) unresolved; isolation and characterization of functionally competent chloroplasts would help in this respect. Endosymbiotic Symbiodiniaceae (in Foraminifera, Acantharia, Radiolaria, Ciliata, Porifera, Acoela, Cnidaria, and Mollusca) obtain inorganic C by transport from seawater through host tissue. In corals this transport apparently provides an inorganic C concentration around the photobiont that obviates the need for photobiont CCM. This is not the case for tridacnid bivalves, medusae, or, possibly, Foraminifera. Overcoming these long-standing knowledge gaps relies on technical advances (e.g., the in vitro kinetics of Form II RuBisCO) that can functionally track the fate of inorganic C forms.
Suggett, DJ & Smith, DJ 2020, 'Coral bleaching patterns are the outcome of complex biological and environmental networking.', Global Change Biology, vol. 26, no. 1, pp. 68-79.View/Download from: Publisher's site
Continued declines in coral reef health over the past three decades have been punctuated by severe mass coral bleaching-induced mortality events that have grown in intensity and frequency under climate change. Intensive global research efforts have therefore persistently focused on bleaching phenomena to understand where corals bleach, when and why-resulting in a large-yet still somewhat patchy-knowledge base. Particularly catastrophic bleaching-induced coral mortality events in the past 5 years have catalyzed calls for a more diverse set of reef management tools, extending far beyond climate mitigation and reef protection, to also include more aggressive interventions. However, the effectiveness of these various tools now rests on rapidly assimilating our knowledge base of coral bleaching into more integrated frameworks. Here, we consider how the past three decades of intensive coral bleaching research has established the basis for complex biological and environmental networks, which together regulate outcomes of bleaching severity. We discuss how we now have enough scaffold for conceptual biological and environmental frameworks underpinning bleaching susceptibility, but that new tools are urgently required to translate this to an operational system informing-and testing-bleaching outcomes. Specifically, adopting network models that can fully describe and predict metabolic functioning of coral holobionts, and how this functioning is regulated by complex doses and interactions among environmental factors. Identifying knowledge gaps limiting operation of such models is the logical step to immediately guide and prioritize future experiments and observations. We are at a time-critical point where we can implement new capacity to resolve how coral bleaching patterns emerge from complex biological-environmental networks, and so more effectively inform rapidly evolving ecological management and social adaptation frameworks aimed at securing the future of coral reefs.
Thackeray, SJ, Robinson, SA, Smith, P, Bruno, R, Kirschbaum, MUF, Bernacchi, C, Byrne, M, Cheung, W, Cotrufo, MF, Gienapp, P, Hartley, S, Janssens, I, Jones, TH, Kobayashi, K, Luo, Y, Penuelas, J, Sage, R, Suggett, DJ, Way, D & Long, S 2020, 'Civil disobedience movements such as School Strike for the Climate are raising public awareness of the climate change emergency', GLOBAL CHANGE BIOLOGY, vol. 26, no. 3, pp. 1042-1044.View/Download from: Publisher's site
Lawson, CA, Seymour, JR, Possell, M, Suggett, DJ & Raina, JB 2020, 'The Volatilomes of Symbiodiniaceae-Associated Bacteria Are Influenced by Chemicals Derived From Their Algal Partner', Frontiers in Marine Science, vol. 7.View/Download from: Publisher's site
© Copyright © 2020 Lawson, Seymour, Possell, Suggett and Raina. Biogenic volatile organic compounds (BVOCs) are a large group of molecules involved in trophic interactions, stress response and atmospheric chemistry. Although they have been extensively studied in terrestrial ecosystems, their identity and prevalence in the marine environment remains largely unexplored. Here we characterized the volatilome of two abundant marine bacteria that were previously identified as members of the core microbiome of Symbiodiniaceae (phylum: Dinoflagellata), the photosynthetic endosymbionts of reef building corals. To determine the influence of Symbiodiniaceae exudate on their associated bacteria, we incubated isolates of Marinobacter adhaerens HP15 and Labrenzia sp. 21p with Symbiodiniaceae culture filtrate or culture medium (control) and investigated their volatilomes using GC–MS. The volatilome of Labrenzia sp. incubated in Symbiodiniaceae filtrate was significantly different and more diverse relative to the control. In contrast, the overall composition of the M. adhaerens volatilomes were consistent between treatment and control. Among the 35 compounds detected in both bacterial species, the dominant chemical functional groups were halogenated hydrocarbons, aromatic hydrocarbons and organosulfurs, some of which are known to play roles in inter-organism signaling, to act as antioxidants and as antimicrobials. This study provides new insights into the potential sources and diversity of marine BVOCs, uncovering a wide range of molecules that may play important physiological and ecological roles for these organisms, while also revealing the role of Symbiodiniaceae-associated bacteria in the emission of important atmospheric gases.
Matthews, JL, Raina, J-B, Kahlke, T, Seymour, JR, van Oppen, MJH & Suggett, DJ 2020, 'Symbiodiniaceae-bacteria interactions: rethinking metabolite exchange in reef-building corals as multi-partner metabolic networks', ENVIRONMENTAL MICROBIOLOGY, vol. 22, no. 5, pp. 1675-1687.View/Download from: Publisher's site
Hughes, DJ, Crosswell, JR, Doblin, MA, Oxborough, K, Ralph, PJ, Varkey, D & Suggett, DJ 2020, 'Dynamic variability of the phytoplankton electron requirement for carbon fixation in eastern Australian waters', Journal of Marine Systems, vol. 202.View/Download from: Publisher's site
© 2019 Elsevier B.V. Fast Repetition Rate fluorometry (FRRf) generates high-resolution measures of phytoplankton primary productivity as electron transport rates (ETRs). How ETRs scale to corresponding inorganic carbon (C) uptake rates (the so-called electron requirement for carbon fixation, Φe,C), inherently describes the extent and effectiveness with which absorbed light energy drives C-fixation. However, it remains unclear whether and how Φe,C follows predictable patterns for oceanographic datasets spanning physically dynamic, and complex, environmental gradients. We utilise a unique high-throughput approach, coupling ETRs and 14C-incubations to produce a semi-continuous dataset of Φe,C (n = 80), predominantly from surface waters, along the Australian coast (Brisbane to the Tasman Sea), including the East Australian Current (EAC). Environmental conditions along this transect could be generally grouped into cooler, more nutrient-rich waters dominated by larger size-fractionated Chl-a (>10 μm) versus warmer nutrient-poorer waters dominated by smaller size-fractionated Chl-a (<2 μm). Whilst Φe,C was higher for warmer water samples, environmental conditions alone explained <20% variance of Φe,C, and changes in predominant size-fraction(s) distributions of Chl-a (biomass) failed to explain variance of Φe,C. Instead, normalised Stern-Volmer non-photochemical quenching (NPQNSV = F0'/Fv') was a better predictor of Φe,C, explaining ~55% of observed variability. NPQNSV is a physiological descriptor that accounts for changes in both long-term driven acclimation in non-radiative decay, and quasi-instantaneous PSII downregulation, and thus may prove a useful predictor of Φe,C across physically-dynamic regimes, provided the slope describing their relationship is predictable. We also consider recent advances in fluorescence-based corrections to evaluate the potential role of baseline fluorescence (Fb) in contributing to overestimation of Φe,C and the correlation between Φe,C...
Hughes, DJ, Giannini, FC, Ciotti, AM, Doblin, MA, Ralph, PJ, Varkey, D, Verma, A & Suggett, DJ 2020, 'Taxonomic variability in the electron requirement for carbon fixation across marine phytoplankton.', Journal of phycology.View/Download from: Publisher's site
Fast Repetition Rate fluorometry (FRRf) has been increasingly used to measure marine primary productivity by oceanographers to understand how carbon (C) uptake patterns vary over space and time in the global oceans. As FRRf measures electron transport rates through photosystem II (ETRPSII ), a critical, but difficult-to-predict conversion factor termed the "electron requirement for carbon fixation" (Φe,C ) is needed to scale ETRPSII to C-fixation rates. Recent studies have generally focused on understanding environmental regulation of Φe,C , while taxonomic control has been explored by only a handful of laboratory studies encompassing a limited diversity of phytoplankton species. We therefore assessed Φe,C for a wide range of marine phytoplankton (n=17 strains) spanning multiple taxonomic and size-classes. Data mined from previous studies were further considered to determine whether Φe,C variability could be explained by taxonomy versus other phenotypic traits influencing growth and physiological performance (e.g., cell size). We found that Φe,C exhibited considerable variability (~4-10 mol e- · [mol C]-1 ), and was negatively correlated with growth rate (R2 = 0.7, p < 0.01). Diatoms exhibited a lower Φe,C compared to chlorophytes during steady-state, nutrient-replete growth. Inclusion of meta-analysis data did not find significant relationships between Φe,C and class, or growth rate, although confounding factors inherent to methodological inconsistencies between studies likely contributed to this. Knowledge of empirical relationships between Φe,C and growth rate coupled with recent improvements in quantifying phytoplankton growth rates in-situ, facilitate up-scaling of FRRf campaigns to routinely derive Φe,C needed to assess ocean C-cycling.
Verma, A, Hughes, DJ, Harwood, DT, Suggett, DJ, Ralph, PJ & Murray, SA 2020, 'Functional significance of phylogeographic structure in a toxic benthic marine microbial eukaryote over a latitudinal gradient along the East Australian Current', Ecology and Evolution.View/Download from: Publisher's site
Camp, EF, Kahlke, T, Nitschke, MR, Varkey, D, Fisher, NL, Fujise, L, Goyen, S, Hughes, DJ, Lawson, CA, Ros, M, Woodcock, S, Xiao, K, Leggat, W & Suggett, DJ 2020, 'Revealing changes in the microbiome of Symbiodiniaceae under thermal stress', ENVIRONMENTAL MICROBIOLOGY, vol. 22, no. 4, pp. 1294-1309.View/Download from: Publisher's site
Camp, EF, Suggett, DJ, Pogoreutz, C, Nitschke, MR, Houlbreque, F, Hume, BCC, Gardner, SG, Zampighi, M, Rodolfo-Metalpa, R & Voolstra, CR 2020, 'Corals exhibit distinct patterns of microbial reorganisation to thrive in an extreme inshore environment', CORAL REEFS, vol. 39, no. 3, pp. 701-716.View/Download from: Publisher's site
Ros, M, Camp, EF, Hughes, DJ, Crosswell, JR, Warner, ME, Leggat, WP & Suggett, DJ 2020, 'Unlocking the black-box of inorganic carbon-uptake and utilization strategies among coral endosymbionts (Symbiodiniaceae)', LIMNOLOGY AND OCEANOGRAPHY, vol. 65, no. 8, pp. 1747-1763.View/Download from: Publisher's site
Suggett, DJ, Edmondson, J, Howlett, L & Camp, EF 2020, 'Coralclip®: a low‐cost solution for rapid and targeted out‐planting of coral at scale', Restoration Ecology.View/Download from: Publisher's site
Re‐attaching or out‐planting coral as fragments, colonies, and on larval settlement devices to substrates is a major bottleneck limiting scalabilty and viability of reef restoration practices. Many attachment approaches are in use, but none that are low‐cost, opportunistic, rapid but effective, for integration into existing tour operations on the Great Barrier Reef (GBR) where staff and boat time is a major cost and chemical fixatives cannot be easily used. We describe a novel attachment device—Coralclip®—developed to meet this need and so aid maintenance and restoration of GBR tourism sites. Coralclip® is a stainless steel springclip attached by a nail integrated through the spring coil, and can be deployed with a coral fragment in as fast as 15 seconds. Initial laboratory tests demonstrated that Coralclip® secured coral fragments or larval settlement tiles under dynamic flow regimes characteristic of exposed reefs. Coral out‐planting from fragments of opportunity and from nurseries (n = 4,580; 0.3–1.9 coral/minute; US$0.6–3.0/coral deployed) or larval settlement tiles (n = 400; 2.5 tiles/minute; US$0.5 tile deployed−1) when deployed by divers from routine boat operations at Opal Reef confirmed highly effective attachment, with ≤15% failure of clips found after 3–7 months. We discuss how Coralclip® is a cost‐effective means to support reef maintenance and restoration practices.
Hughes, DJ, Alderdice, R, Cooney, C, Kuehl, M, Pernice, M, Voolstra, CR & Suggett, DJ 2020, 'Coral reef survival under accelerating ocean deoxygenation', NATURE CLIMATE CHANGE, vol. 10, no. 4, pp. 296-307.View/Download from: Publisher's site
Zhu, Y, Suggett, DJ, Liu, C, He, J, Lin, L, Le, F, Ishizaka, J, Goes, J & Hao, Q 2019, 'Primary Productivity Dynamics in the Summer Arctic Ocean Confirms Broad Regulation of the Electron Requirement for Carbon Fixation by Light-Phytoplankton Community Interaction', Frontiers in Marine Science, vol. 6.View/Download from: Publisher's site
Akyol, S, Ben Nissan, B, Karacan, I, Yetmez, M, Gokce, H, Suggett, DJ & Oktar, FN 2019, 'Morphology, characterization, and conversion of the corals Goniopora spp. and Porites cylindrica to hydroxyapatite', Journal of the Australian Ceramic Society, vol. 55, no. 3, pp. 893-901.View/Download from: Publisher's site
© 2019, Australian Ceramic Society. The aim of this study is to obtain pure natural hydroxyapatite (HAp) and tricalcium phosphate (TCP) from a Goniopora spp. and from hump coral (Porites cylindrica), both sourced from Australia. Due to the nature of the conversion process, commercial coralline HAp has retained coral or CaCO3, and the structure possesses both nano- and mesopores within the interpore trabeculae resulting in high dissolution rates. To overcome these limitations, a newly patented coral double-conversion technique has been developed. The current technique involves a two-stage application route where in the first-stage complete conversion of coral to pure HAp is achieved. In the second stage, a sol-gel-derived HAp nanocoating is directly applied to cover the meso- and nanopores within the intrapore material, while maintaining the large pores. Here, we specifically investigated the morphological changes and characterized these corals prior to and after conversion. For this purpose, four groups designated as C0, C1, C2, and C3 were used. C0 is Porites, Goniopora, and cylindrica; the original coral is calcium carbonate with aragonite structure that contains proteins and polysaccharides. C1 is coral cleaned under ultrasound in bleach diluted with water. C2 is coral converted to hydroxyapatite (HAp) by hydrothermal treatment method at 200 °C under pressure in the presence of ammonium biphosphate. C3 is obtained by coating C2 with sol-gel alkoxide-derived nanohydroxyapatite to obtain a more bioactive osteoconductive material and improve mechanical properties. All groups were characterized by XRD, EDAX, DTA/TGA, and SEM. The results showed that the biaxial strengths of the C2 and C3 were significantly higher than the original coral. The work also showed the advantages of the hydrothermal conversion method and the effect of the nanocoating which is expected to improve the final bioactivity through microstructural changes of the surfaces.
Bretherton, L, Poulton, AJ, Lawson, T, Rukminasari, N, Balestreri, C, Schroeder, D, Mark Moore, C & Suggett, DJ 2019, 'Day length as a key factor moderating the response of coccolithophore growth to elevated pCO 2', Limnology and Oceanography, vol. 64, no. 3, pp. 1284-1296.View/Download from: Publisher's site
© 2019 The Authors. Limnology and Oceanography published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography. The fate of coccolithophores in the future oceans remains uncertain, in part due to key factors having not been standardized across experiments. A potentially moderating role for differences in day length (photoperiod) remains largely unexplored. We therefore cultured four different geographical isolates of the species Emiliania huxleyi, as well as two additional species, Gephyrocapsa oceanica (tropical) and Coccolithus braarudii (temperate), to test for interactive effects of pCO 2 with the light : dark (L : D) cycle. We confirmed a general regulatory effect of photoperiod on the pCO 2 response, whereby growth and particulate inorganic carbon and particulate organic carbon (PIC : POC) ratios were reduced with elevated pCO 2 under 14 : 10 h L : D, but these reductions were dampened under continuous (24 h) light. The dynamics underpinning this pattern generally differed for the temperate vs. tropical isolates. Reductions in PIC : POC with elevated pCO 2 for tropical taxa were largely through reduced calcification and enhanced photosynthesis under 14 : 10 h L : D, with differences dampened under continuous light. In contrast, reduced PIC : POC for temperate strains reflected increases of photosynthesis that outpaced increases in calcification rates under 14 : 10 h L : D, with both responses again dampened under continuous light. A multivariate analysis of 35 past studies of E. huxleyi further demonstrated that differences in photoperiod account for as much as 40% (strain B11/92) to 55% (strain NZEH) of the variance in reported pCO 2 -induced reductions to growth but not PIC : POC. Our study thus highlights a critical role for day length in moderating the effect of ocean acidification on coccolithophore growth and consequently how this response may play out across latitudes and seasons in future oceans.
Seguro, I, Marca, AD, Painting, SJ, Shutler, JD, Suggett, DJ & Kaiser, J 2019, 'High-resolution net and gross biological production during a Celtic Sea spring bloom', Progress in Oceanography, vol. 177.View/Download from: Publisher's site
© 2017 The Authors Shelf seas represent only 10% of the ocean area, but support up to 30% of all oceanic primary production. There are few measurements of shelf-sea biological production at high spatial and temporal resolution in such heterogeneous and physically dynamic systems. Here, we use dissolved oxygen-to-argon (O2/Ar) ratios and oxygen triple isotopes (16O, 17O, 18O) to estimate net and gross biological production in the Celtic Sea during spring 2015. O2/Ar ratios were measured continuously using a shipboard membrane inlet mass spectrometer (MIMS). Additional discrete water samples from CTD hydrocasts were used to measure O2/Ar depth profiles and the δ(17O) and δ(18O) values of dissolved O2. These high-resolution data were combined with wind-speed based gas exchange parameterisations to calculate biologically driven air-sea oxygen fluxes. After correction for disequilibrium terms and diapycnal diffusion, these fluxes yielded estimates of net community (N(O2/Ar)) and gross O2 production (G(17O)). N(O2/Ar) was spatially heterogeneous and showed predominantly autotrophic conditions, with an average of (33 ± 41) mmol m−2 d−1. G(17O) showed high variability between 0 and 424 mmol m−2 d−1. The ratio of N(O2/Ar) to G(17O), ƒ(O2), was (0.18 ± 0.03) corresponding to 0.34 ± 0.06 in carbon equivalents. We also observed rapid temporal changes in N(O2/Ar), e.g. an increase of 80 mmol m−2 d−1 in <6 h during the spring bloom, highlighting the importance of high-resolution biological production measurements. Such measurements will help reconcile the differences between satellite and in situ productivity observations, and improve our understanding of the biological carbon pump.
Zhu, Y, Suggett, DJ, Liu, C, He, J, Lin, L, Le, F, Ishizaka, J, Goes, J & Hao, Q 2019, 'Primary Productivity Dynamics in the Summer Arctic Ocean Confirms Broad Regulation of the Electron Requirement for Carbon Fixation by Light-Phytoplankton Community Interaction', Frontiers in Marine Science, vol. 6.View/Download from: Publisher's site
Lawson, CA, Possell, M, Seymour, JR, Raina, J-B & Suggett, DJ 2019, 'Coral endosymbionts (Symbiodiniaceae) emit species-specific volatilomes that shift when exposed to thermal stress.', Scientific reports, vol. 9, no. 1.View/Download from: Publisher's site
Biogenic volatile organic compounds (BVOCs) influence organism fitness by promoting stress resistance and regulating trophic interactions. Studies examining BVOC emissions have predominantly focussed on terrestrial ecosystems and atmospheric chemistry - surprisingly, highly productive marine ecosystems remain largely overlooked. Here we examined the volatilome (total BVOCs) of the microalgal endosymbionts of reef invertebrates, Symbiodiniaceae. We used GC-MS to characterise five species (Symbiodinium linucheae, Breviolum psygmophilum, Durusdinium trenchii, Effrenium voratum, Fugacium kawagutii) under steady-state growth. A diverse range of 32 BVOCs were detected (from 12 in D. trenchii to 27 in S. linucheae) with halogenated hydrocarbons, alkanes and esters the most common chemical functional groups. A thermal stress experiment on thermally-sensitive Cladocopium goreaui and thermally-tolerant D. trenchii significantly affected the volatilomes of both species. More BVOCs were detected in D. trenchii following thermal stress (32 °C), while fewer BVOCs were recorded in stressed C. goreaui. The onset of stress caused dramatic increases of dimethyl-disulfide (98.52%) in C. goreaui and nonanoic acid (99.85%) in D. trenchii. This first volatilome analysis of Symbiodiniaceae reveals that both species-specificity and environmental factors govern the composition of BVOC emissions among the Symbiodiniaceae, which potentially have, as yet unexplored, physiological and ecological importance in shaping coral reef community functioning.
Gardner, SG, Camp, EF, Smith, DJ, Kahlke, T, Osman, EO, Gendron, G, Hume, BCC, Pogoreutz, C, Voolstra, CR & Suggett, DJ 2019, 'Coral microbiome diversity reflects mass coral bleaching susceptibility during the 2016 El Niño heat wave.', Ecology and Evolution, vol. 9, no. 3, pp. 938-956.View/Download from: Publisher's site
Repeat marine heat wave-induced mass coral bleaching has decimated reefs in Seychelles for 35 years, but how coral-associated microbial diversity (microalgal endosymbionts of the family Symbiodiniaceae and bacterial communities) potentially underpins broad-scale bleaching dynamics remains unknown. We assessed microbiome composition during the 2016 heat wave peak at two contrasting reef sites (clear vs. turbid) in Seychelles, for key coral species considered bleaching sensitive (Acropora muricata, Acropora gemmifera) or tolerant (Porites lutea, Coelastrea aspera). For all species and sites, we sampled bleached versus unbleached colonies to examine how microbiomes align with heat stress susceptibility. Over 30% of all corals bleached in 2016, half of which were from Acropora sp. and Pocillopora sp. mass bleaching that largely transitioned to mortality by 2017. Symbiodiniaceae ITS2-sequencing revealed that the two Acropora sp. and P. lutea generally associated with C3z/C3 and C15 types, respectively, whereas C. aspera exhibited a plastic association with multiple D types and two C3z types. 16S rRNA gene sequencing revealed that bacterial communities were coral host-specific, largely through differences in the most abundant families, Hahellaceae (comprising Endozoicomonas), Rhodospirillaceae, and Rhodobacteraceae. Both Acropora sp. exhibited lower bacterial diversity, species richness, and community evenness compared to more bleaching-resistant P. lutea and C. aspera. Different bleaching susceptibility among coral species was thus consistent with distinct microbiome community profiles. These profiles were conserved across bleached and unbleached colonies of all coral species. As this pattern could also reflect a parallel response of the microbiome to environmental changes, the detailed functional associations will need to be determined in future studies. Further understanding such microbiome-environmental interactions is likely critical to target more effective manag...
Goyen, S, Camp, EF, Fujise, L, Lloyd, A, Nitschke, MR, LaJeunensse, T, Kahlke, T, Ralph, PJ & Suggett, D 2019, 'Mass coral bleaching of P. versipora in Sydney Harbour driven by the 2015–2016 heatwave', Coral Reefs, vol. 38, no. 4, pp. 815-830.View/Download from: Publisher's site
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. High-latitude coral communities are distinct from their tropical counterparts, and how they respond to recent heat wave events that have decimated tropical reefs remains unknown. In Australia, the 2016 El Niño resulted in the largest global mass coral bleaching event to date, reaching as far south as Sydney Harbour (~ 34°S). Coral bleaching was observed for the first time (affecting ca., 60% of all corals) as sea surface temperatures in Sydney Harbour remained > 2 °C above the long-term mean summer maxima, enabling us to examine whether high-latitude corals bleached in a manner described for tropical corals. Responses of the geographically cosmopolitan Plesiastrea versipora and southerly restricted Coscinaraea mcneilli were contrasted across two harbour sites, both in situ and among samples-maintained ex situ in aquaria continually supplied with Sydney Harbour seawater. While both coral taxa hosted the same species of microalgal endosymbiont (Breviolum spp; formerly clade B), only P. versipora bleached both in situ and ex situ via pronounced losses of endosymbiont cells. Both species displayed very different metabolic responses (growth, photosynthesis, respiration and calcification) and bleaching susceptibilities under elevated temperatures. Bacterial microbiome profiling, however, revealed a convergence of bacterial community composition across coral species throughout the bleaching. Corals species found in temperate regions, including the generalist P. versipora, will therefore likely be highly susceptible to future change as heat waves grow in frequency and severity unless their thermal thresholds increase. Our observations provide further evidence that high-latitude systems are susceptible to community reorganisation under climate change.
Leggat, WP, Camp, EF, Suggett, DJ, Heron, SF, Fordyce, AJ, Gardner, S, Deakin, L, Turner, M, Beeching, LJ, Kuzhiumparambil, U, Eakin, CM & Ainsworth, TD 2019, 'Rapid Coral Decay Is Associated with Marine Heatwave Mortality Events on Reefs.', Current biology : CB, vol. 29, no. 16, pp. 2723-2730.View/Download from: Publisher's site
Severe marine heatwaves have recently become a common feature of global ocean conditions due to a rapidly changing climate [1, 2]. These increasingly severe thermal conditions are causing an unprecedented increase in the frequency and severity of mortality events in marine ecosystems, including on coral reefs . The degradation of coral reefs will result in the collapse of ecosystem services that sustain over half a billion people globally [4, 5]. Here, we show that marine heatwave events on coral reefs are biologically distinct to how coral bleaching has been understood to date, in that heatwave conditions result in an immediate heat-induced mortality of the coral colony, rapid coral skeletal dissolution, and the loss of the three-dimensional reef structure. During heatwave-induced mortality, the coral skeletons exposed by tissue loss are, within days, encased by a complex biofilm of phototrophic microbes, whose metabolic activity accelerates calcium carbonate dissolution to rates exceeding accretion by healthy corals and far greater than has been documented on reefs under normal seawater conditions. This dissolution reduces the skeletal density and hardness and increases porosity. These results demonstrate that severe-heatwave-induced mortality events should be considered as a distinct biological phenomenon from bleaching events on coral reefs. We also suggest that such heatwave mortality events, and rapid reef decay, will become more frequent as the intensity of marine heatwaves increases and provides further compelling evidence for the need to mitigate climate change and instigate actions to reduce marine heatwaves.
Lohr, KE, Camp, EF, Kuzhiumparambil, U, Lutz, A, Leggat, W, Patterson, JT & Suggett, DJ 2019, 'Resolving coral photoacclimation dynamics through coupled photophysiological and metabolomic profiling.', The Journal of experimental biology, vol. 222, no. Pt 8.View/Download from: Publisher's site
Corals continuously adjust to short-term variation in light availability on shallow reefs. Long-term light alterations can also occur as a result of natural and anthropogenic stressors, as well as management interventions such as coral transplantation. Although short-term photophysiological responses are relatively well understood in corals, little information is available regarding photoacclimation dynamics over weeks of altered light availability. We coupled photophysiology and metabolomic profiling to explore changes that accompany longer-term photoacclimation in a key Great Barrier Reef coral species, Acropora muricata High light (HL)- and low light (LL)-acclimated corals were collected from the reef and reciprocally exposed to high and low light ex situ Rapid light curves using pulse-amplitude modulation (PAM) fluorometry revealed photophysiological acclimation of LL corals to HL and HL corals to LL within 21 days. A subset of colonies sampled at 7 and 21 days for untargeted LC-MS and GC-MS metabolomic profiling revealed metabolic reorganization before acclimation was detected using PAM fluorometry. Metabolomic shifts were more pronounced for LL to HL corals than for their HL to LL counterparts. Compounds driving metabolomic separation between HL-exposed and LL control colonies included amino acids, organic acids, fatty acids and sterols. Reduced glycerol and campesterol suggest decreased translocation of photosynthetic products from symbiont to host in LL to HL corals, with concurrent increases in fatty acid abundance indicating reliance on stored lipids for energy. We discuss how these data provide novel insight into environmental regulation of metabolism and implications for management strategies that drive rapid changes in light availability.
Suggett, DJ, Camp, EF, Edmondson, J, Boström-Einarsson, L, Ramler, V, Lohr, K & Patterson, JT 2019, 'Optimizing return-on-effort for coral nursery and outplanting practices to aid restoration of the Great Barrier Reef', Restoration Ecology, vol. 27, no. 3, pp. 683-693.View/Download from: Publisher's site
© 2018 Society for Ecological Restoration Coral nursery and outplanting practices have grown in popularity worldwide for targeted restoration of degraded "high value" reef sites, and recovery of threatened taxa. Success of these practices is commonly gauged from coral propagule growth and survival, which fundamentally determines the return-on-effort (RRE) critical to the cost-effectiveness and viability of restoration programs. In many cases, RRE has been optimized from past successes and failures, which therefore presents a major challenge for locations such as the Great Barrier Reef (GBR) where no local history of restoration exists to guide best practice. In establishing the first multi-taxa coral nursery on the GBR (Opal Reef, February 2018), we constructed a novel scoring criterion from concurrent measurements of growth and survivorship to guide our relative RRE, including nursery propagule numbers (stock density). We initially retrieved RRE scores from a database of global restoration efforts to date (n = 246; 52 studies) to evaluate whether and how success commonly varied among coral taxa. We then retrieved RRE scores for Opal Reef using initial growth and survivorship data for six key coral taxa, to demonstrate that RRE scores were high for all taxa predominantly via high survivorship over winter. Repeated RRE scoring in summer is therefore needed to capture the full dynamic range of success where seasonal factors regulating growth versus survivorship differ. We discuss how RRE scoring can be easily adopted across restoration practices globally to standardize and benchmark success, but also as a tool to aid decision-making in optimizing future propagation (and outplanting) efforts.
Camp, EF, Edmondson, J, Doheny, A, Rumney, J, Grima, AJ, Huete, A & Suggett, DJ 2019, 'Mangrove lagoons of the Great Barrier Reef support coral populations persisting under extreme environmental conditions', Marine Ecology Progress Series, vol. 625, pp. 1-14.View/Download from: Publisher's site
© The authors 2019. Global degradation of coral reefs has increased the urgency of identifying stress-tolerant coral populations, to enhance understanding of the biology driving stress tolerance, as well as identifying stocks of stress-hardened populations to aid reef rehabilitation. Surprisingly, scientists are continually discovering that naturally extreme environments house established coral populations adapted to grow within extreme abiotic conditions comparable to seawater conditions predicted over the coming century. Such environments include inshore mangrove lagoons that carry previously unrecognised ecosystem service value for corals, spanning from refuge to stress preconditioning. However, the existence of such hot-spots of resilience on the Great Barrier Reef (GBR) remains entirely unknown. Here we describe, for the first time, 2 extreme GBR mangrove lagoons (Woody Isles and Howick Island), exposing taxonomically diverse coral communities (34 species, 7 growth morphologies) to regular extreme low pH (<7.6), low oxygen (<1 mg l−1) and highly variable temperature range (>7°C) conditions. Coral cover was typically low (<5%), but highly patchy and included established colonies (>0.5 m diameter), with net photosynthesis and calcification rates of 2 dominant coral species (Acropora millepora, Porites lutea) reduced (20−30%), and respiration enhanced (11−35%), in the mangrove lagoon relative to adjacent reefs. Further analysis revealed that physiological plasticity (photosynthetic 'strategy') and flexibility of Symbiodiniaceae taxa associations appear crucial in supporting coral capacity to thrive from reef to lagoon. Prevalence of corals within these extreme conditions on the GBR (and elsewhere) increasingly challenge our understanding of coral resilience to stressors, and highlight the need to study unfavourable coral environments to better resolve mechanisms of stress tolerance.
Osman, EO, Smith, DJ, Ziegler, M, Kürten, B, Conrad, C, El-Haddad, KM, Voolstra, CR & Suggett, DJ 2018, 'Thermal refugia against coral bleaching throughout the northern Red Sea.', Global Change Biology, vol. 24, no. 2, pp. e474-e484.View/Download from: Publisher's site
Tropical reefs have been impacted by thermal anomalies caused by global warming that induced coral bleaching and mortality events globally. However, there have only been very few recordings of bleaching within the Red Sea despite covering a latitudinal range of 15° and consequently it has been considered a region that is less sensitive to thermal anomalies. We therefore examined historical patterns of sea surface temperature (SST) and associated anomalies (1982-2012) and compared warming trends with a unique compilation of corresponding coral bleaching records from throughout the region. These data indicated that the northern Red Sea has not experienced mass bleaching despite intensive Degree Heating Weeks (DHW) of >15°C-weeks. Severe bleaching was restricted to the central and southern Red Sea where DHWs have been more frequent, but far less intense (DHWs <4°C-weeks). A similar pattern was observed during the 2015-2016 El Niño event during which time corals in the northern Red Sea did not bleach despite high thermal stress (i.e. DHWs >8°C-weeks), and bleaching was restricted to the central and southern Red Sea despite the lower thermal stress (DHWs < 8°C-weeks). Heat stress assays carried out in the northern (Hurghada) and central (Thuwal) Red Sea on four key reef-building species confirmed different regional thermal susceptibility, and that central Red Sea corals are more sensitive to thermal anomalies as compared to those from the north. Together, our data demonstrate that corals in the northern Red Sea have a much higher heat tolerance than their prevailing temperature regime would suggest. In contrast, corals from the central Red Sea are close to their thermal limits, which closely match the maximum annual water temperatures. The northern Red Sea harbours reef-building corals that live well below their bleaching thresholds and thus we propose that the region represents a thermal refuge of global importance.
Richier, S, Achterberg, EP, Humphreys, MP, Poulton, AJ, Suggett, DJ, Tyrrell, T & Moore, CM 2018, 'Geographical CO2 sensitivity of phytoplankton correlates with ocean buffer capacity.', Global Change Biology, vol. 24, no. 9, pp. 4438-4452.View/Download from: Publisher's site
Accumulation of anthropogenic CO2 is significantly altering ocean chemistry. A range of biological impacts resulting from this oceanic CO2 accumulation are emerging, however, the mechanisms responsible for observed differential susceptibility between organisms and across environmental settings remain obscure. A primary consequence of increased oceanic CO2 uptake is a decrease in the carbonate system buffer capacity, which characterizes the system's chemical resilience to changes in CO2 , generating the potential for enhanced variability in pCO2 and the concentration of carbonate [CO32-], bicarbonate [HCO3-], and protons [H+ ] in the future ocean. We conducted a meta-analysis of 17 shipboard manipulation experiments performed across three distinct geographical regions that encompassed a wide range of environmental conditions from European temperate seas to Arctic and Southern oceans. These data demonstrated a correlation between the magnitude of natural phytoplankton community biological responses to short-term CO2 changes and variability in the local buffer capacity across ocean basin scales. Specifically, short-term suppression of small phytoplankton (<10 μm) net growth rates were consistently observed under enhanced pCO2 within experiments performed in regions with higher ambient buffer capacity. The results further highlight the relevance of phytoplankton cell size for the impacts of enhanced pCO2 in both the modern and future ocean. Specifically, cell size-related acclimation and adaptation to regional environmental variability, as characterized by buffer capacity, likely influences interactions between primary producers and carbonate chemistry over a range of spatio-temporal scales.
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Corals synthesise large quantities of the sulphur metabolite dimethylsulphoniopropionate (DMSP), which contributes to key roles in coral reef ecology including the capacity of corals to withstand various stressors. While closely related to scleractinian corals and often occupying similar ecological niche space, it is currently poorly defined to what extent soft corals produce DMSP. We, therefore, examined DMSP content within four key species of soft coral in February and July–August of 2017, including two temperate species from Sydney Harbour (Erythropodium hicksoni, Capnella gaboensis) and two tropical species from the Great Barrier Reef (Sinularia sp., Sarcophyton sp.). We compared DMSP content of these soft coral species to that of commonly occurring temperate (Plesiastrea versipora) and tropical (Acropora aspera) scleractinian coral species. DMSP content was normalised to coral protein content, with soft coral DMSP content highly variable across species and locations [56–539 nmol (mg protein)−1], and lower than for the tropical [1242–4710 nmol (mg protein)−1], but not temperate [465–1984 nmol (mg protein)−1] scleractinian species. Further comparison with previously published values demonstrated that soft coral DMSP content falls within the "low–mid range" of scleractinian corals. Notably, DMSP content was also higher in summer samples than winter samples for the scleractinian corals, but did not differ between seasons for soft corals. Such contrasting dynamics of DMSP production by soft corals compared to scleractinian corals indicates that the regulation of DMSP content differs between these two important benthic cnidarian groups, potentially as a consequence of dissimilar ecophysiological roles for this compound.
Lawson, CA, Raina, J-B, Kahlke, T, Seymour, JR & Suggett, DJ 2018, 'Defining the core microbiome of the symbiotic dinoflagellate, Symbiodinium.', Environmental microbiology reports, vol. 10, no. 1, pp. 7-11.View/Download from: Publisher's site
Dinoflagellates of the genus Symbiodinium underpin the survival and ecological success of corals. The use of cultured strains has been particularly important to disentangle the complex life history of Symbiodinium and their contribution to coral host physiology. However, these cultures typically harbour abundant bacterial communities which likely play important, but currently unknown, roles in Symbiodinium biology. We characterized the bacterial communities living in association with a wide phylogenetic diversity of Symbiodinium cultures (18 types spanning 5 clades) to define the core Symbiodinium microbiome. Similar to other systems, bacteria were nearly two orders of magnitude more numerically abundant than Symbiodinium cells and we identified three operational taxonomic units (OTUs) which were present in all cultures. These represented the α-proteobacterium Labrenzia and the γ-proteobacteria Marinobacter and Chromatiaceae. Based on the abundance and functional potential of bacteria harboured in these cultures, their contribution to Symbiodinium physiology can no longer be ignored.
Fujise, L, Nitschke, MR, Frommlet, JC, Serôdio, J, Woodcock, S, Ralph, PJ & Suggett, DJ 2018, 'Cell Cycle Dynamics of Cultured Coral Endosymbiotic Microalgae (Symbiodinium) Across Different Types (Species) Under Alternate Light and Temperature Conditions', Journal of Eukaryotic Microbiology, vol. 65, pp. 505-517.View/Download from: Publisher's site
Hughes, DJ, Campbell, DA, Doblin, MA, Kromkamp, JC, Lawrenz, E, Moore, CM, Oxborough, K, Prášil, O, Ralph, PJ, Alvarez, MF & Suggett, DJ 2018, 'Roadmaps and Detours: Active Chlorophyll- a Assessments of Primary Productivity Across Marine and Freshwater Systems.', Environmental science & technology, vol. 52, pp. 12039-12054.View/Download from: Publisher's site
Assessing phytoplankton productivity over space and time remains a core goal for oceanographers and limnologists. Fast Repetition Rate fluorometry (FRRf) provides a potential means to realize this goal with unprecedented resolution and scale yet has not become the "go-to" method despite high expectations. A major obstacle is difficulty converting electron transfer rates to equivalent rates of C-fixation most relevant for studies of biogeochemical C-fluxes. Such difficulty stems from methodological inconsistencies and our limited understanding of how the electron requirement for C-fixation (Φe,C) is influenced by the environment and by differences in the composition and physiology of phytoplankton assemblages. We outline a "roadmap" for limiting methodological bias and to develop a more mechanistic understanding of the ecophysiology underlying Φe,C. We 1) re-evaluate core physiological processes governing how microalgae invest photosynthetic electron transport-derived energy and reductant into stored carbon versus alternative sinks. Then, we 2) outline steps to facilitate broader uptake and exploitation of FRRf, which could transform our knowledge of aquatic primary productivity. We argue it is time to 3) revise our historic methodological focus on carbon as the currency of choice, to 4) better appreciate that electron transport fundamentally drives ecosystem biogeochemistry, modulates cell-to-cell interactions, and ultimately modifies community biomass and structure.
Hughes, DJ, Varkey, D, Doblin, MA, Ingleton, T, Mcinnes, A, Ralph, PJ, van Dongen-Vogels, V & Suggett, DJ 2018, 'Impact of nitrogen availability upon the electron requirement for carbon fixation in Australian coastal phytoplankton communities', Limnology and Oceanography, vol. 63, no. 5, pp. 1891-1910.View/Download from: Publisher's site
© 2018 Association for the Sciences of Limnology and Oceanography Nitrogen (N) availability affects phytoplankton photosynthetic performance and regulates marine primary production (MPP) across the global coast and oceans. Bio-optical tools including Fast Repetition Rate fluorometry (FRRf) are particularly well suited to examine MPP variability in coastal regions subjected to dynamic spatio-temporal fluctuations in nutrient availability. FRRf determines photosynthesis as an electron transport rate through Photosystem II (ETRPSII), requiring knowledge of an additional parameter, the electron requirement for carbon fixation (KC), to retrieve rates of CO2-fixation. KC strongly depends upon environmental conditions regulating photosynthesis, yet the importance of N-availability to this parameter has not been examined. Here, we use nutrient bioassays to isolate how N (relative to other macronutrients P, Si) regulates KC of phytoplankton communities from the Australian coast during summer, when N-availability is often highly variable. KC consistently responded to N-amendment, exhibiting up to a threefold reduction and hence an apparent increase in the efficiency with which electrons were used to drive C-fixation. However, the process driving this consistent reduction was dependent upon initial conditions. When diatoms dominated assemblages and N was undetectable (e.g., post bloom), KC decreased predominantly via a physiological adjustment of the existing community to N-amendment. Conversely, for mixed assemblages, N-addition achieved a similar reduction in KC through a change in community structure toward diatom domination. We generate new understanding and parameterization of KC that is particularly critical to advance how FRRf can be applied to examine C-uptake throughout the global ocean where nitrogen availability is highly variable and thus frequently limits primary productivity.
Camp, EF, Schoepf, V & Suggett, DJ 2018, 'How can "Super Corals" facilitate global coral reef survival under rapid environmental and climatic change?', Global change biology, vol. 24, no. 7, pp. 2755-2757.View/Download from: Publisher's site
Coral reefs are in a state of rapid global decline via environmental and climate change, and efforts have intensified to identify or engineer coral populations with increased resilience. Concurrent with these efforts has been increasing use of the popularized term "Super Coral" in both popular media and scientific literature without a unifying definition. However, how this subjective term is currently applied has the potential to mislead inference over factors contributing to coral survivorship, and the future trajectory of coral reef form and functioning. Here, we discuss that the information required to support a single definition does not exist, and in fact may never be appropriate, i.e. "How Super is Super"? Instead, we advocate caution of this term, and suggest a workflow that enables contextualization and clarification of superiority to ensure that inferred or asserted survivorship is appropriate into future reef projections. This is crucial to robustly unlock how "Super Corals" can be integrated into the suite of management options required to facilitate coral survival under rapid environmental and climate change.
Camp, EF, Schoepf, V, Mumby, PJ & Suggett, DJ 2018, 'The Future of Coral Reefs Subject to Rapid Climate Change: Lessons From Natural Extreme Environments', FRONTIERS IN MARINE SCIENCE, vol. 5.View/Download from: Publisher's site
Camp, EF, Schoepf, V, Mumby, PJ, Hardtke, LA, Rodolfo-Metalpa, R, Smith, DJ & Suggett, DJ 2018, 'The Future of Coral Reefs Subject to Rapid Climate Change: Lessons from Natural Extreme Environments', FRONTIERS IN MARINE SCIENCE, vol. 5.View/Download from: Publisher's site
Nitschke, MR, Gardner, SG, Goyen, S, Fujise, L, Camp, EF, Ralph, PJ & Suggett, DJ 2018, 'Utility of photochemical traits as diagnostics of thermal tolerance amongst great barrier reef corals', Frontiers in Marine Science, vol. 5, no. FEB.View/Download from: Publisher's site
© 2018 Nitschke, Gardner, Goyen, Fujise, Camp, Ralph and Suggett. Light availability is considered a key factor regulating the thermal sensitivity of reef building corals, where excessive excitation of photosystem II (PSII) further exacerbates pressure on photochemical pathways already compromised by heat stress. Coral symbionts acclimate to changes in light availability (photoacclimation) by continually fine-tuning the photochemical operating efficiency of PSII. However, how this process adjusts throughout the warmest months in naturally heat-tolerant or sensitive species is unknown, and whether this influences the capacity to tolerate transient heat stress is untested. We therefore examined the PSII photophysiology of 10 coral species (with known thermal tolerances) from shallow reef environments at Heron Island (Great Barrier Reef, Australia), in spring (October-November, 2015) vs. summer (February-March, 2016). Corals were maintained in flow-through aquaria and rapid light curve (RLC) protocols using pulse amplitude modulated (PAM) fluorometry captured changes in the PSII photoacclimation strategy, characterized as the minimum saturating irradiance (Ek), and the extent of photochemical ([1-C], operating efficiency) vs. non-photochemical ([1-Q]) energy dissipation. Values of Ekacross species were > 2-fold higher in all coral species in spring, consistent with a climate of higher overall light exposure (i.e., higher PAR from lower cloud cover, rainfall and wind speed) compared with summer. Summer decreases in Ekwere combined with a shift toward preferential photochemical quenching in all species. All coral species were subsequently subjected to thermal stress assays. An equivalent temperature-ramping profile of 1°C increase per day and then maintenance at 32°C was applied in each season. Despite the significant seasonal photoacclimation, the species hierarchy of thermal tolerance [maximum quantum yields of PSII (Fv/Fm), monitored at dawn and dusk] did not shift...
Anthony, K, Bay, LK, Costanza, R, Firn, J, Gunn, J, Harrison, P, Heyward, A, Lundgren, P, Mead, D, Moore, T, Mumby, PJ, van Oppen, MJH, Robertson, J, Runge, MC, Suggett, DJ, Schaffelke, B, Wachenfeld, D & Walshe, T 2017, 'New interventions are needed to save coral reefs.', Nature Ecology and Evolution, vol. 1, no. 10, pp. 1420-1422.View/Download from: Publisher's site
Klein, SG, Pitt, KA, Nitschke, MR, Goyen, S, Welsh, DT, Suggett, DJ & Carroll, AR 2017, 'Symbiodinium mitigate the combined effects of hypoxia and acidification on a noncalcifying cnidarian.', Global Change Biology, vol. 23, no. 9, pp. 3690-3703.View/Download from: Publisher's site
Anthropogenic nutrient inputs enhance microbial respiration within many coastal ecosystems, driving concurrent hypoxia and acidification. During photosynthesis, Symbiodinium spp., the microalgal endosymbionts of cnidarians and other marine phyla, produce O2 and assimilate CO2 and thus potentially mitigate the exposure of the host to these stresses. However, such a role for Symbiodinium remains untested for noncalcifying cnidarians. We therefore contrasted the fitness of symbiotic and aposymbiotic polyps of a model host jellyfish (Cassiopea sp.) under reduced O2 (~2.09 mg/L) and pH (~ 7.63) scenarios in a full-factorial experiment. Host fitness was characterized as asexual reproduction and their ability to regulate internal pH and Symbiodinium performance characterized by maximum photochemical efficiency, chla content and cell density. Acidification alone resulted in 58% more asexual reproduction of symbiotic polyps than aposymbiotic polyps (and enhanced Symbiodinium cell density) suggesting Cassiopea sp. fitness was enhanced by CO2 -stimulated Symbiodinium photosynthetic activity. Indeed, greater CO2 drawdown (elevated pH) was observed within host tissues of symbiotic polyps under acidification regardless of O2 conditions. Hypoxia alone produced 22% fewer polyps than ambient conditions regardless of acidification and symbiont status, suggesting Symbiodinium photosynthetic activity did not mitigate its effects. Combined hypoxia and acidification, however, produced similar numbers of symbiotic polyps compared with aposymbiotic kept under ambient conditions, demonstrating that the presence of Symbiodinium was key for mitigating the combined effects of hypoxia and acidification on asexual reproduction. We hypothesize that this mitigation occurred because of reduced photorespiration under elevated CO2 conditions where increased net O2 production ameliorates oxygen debt. We show that Symbiodinium play an important role in facilitating enhanced fitness of Cassiopea sp. ...
Levin, RA, Suggett, DJ, Nitschke, MR, van Oppen, MJH & Steinberg, PD 2017, 'Expanding the Symbiodinium (Dinophyceae, Suessiales) Toolkit Through Protoplast Technology.', The Journal of Eukaryotic Microbiology, vol. 64, no. 5, pp. 588-597.View/Download from: Publisher's site
Dinoflagellates within the genus Symbiodinium are photosymbionts of many tropical reef invertebrates, including corals, making them central to the health of coral reefs. Symbiodinium have therefore gained significant research attention, though studies have been constrained by technical limitations. In particular, the generation of viable cells with their cell walls removed (termed protoplasts) has enabled a wide range of experimental techniques for bacteria, fungi, plants, and algae such as ultrastructure studies, virus infection studies, patch clamping, genetic transformation, and protoplast fusion. However, previous studies have struggled to remove the cell walls from armored dinoflagellates, potentially due to the internal placement of their cell walls. Here, we produce the first Symbiodinium protoplasts from three genetically and physiologically distinct strains via incubation with cellulase and osmotic agents. Digestion of the cell walls was verified by a lack of Calcofluor White fluorescence signal and by cell swelling in hypotonic culture medium. Fused protoplasts were also observed, motivating future investigation into intra- and inter-specific somatic hybridization of Symbiodinium. Following digestion and transfer to regeneration medium, protoplasts remained photosynthetically active, regrew cell walls, regained motility, and entered exponential growth. Generation of Symbiodinium protoplasts opens exciting, new avenues for researching these crucial symbiotic dinoflagellates, including genetic modification.
Levin, RA, Voolstra, CR, Agrawal, S, Steinberg, PD, Suggett, DJ & van Oppen, MJH 2017, 'Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts.', Frontiers in Microbiology, vol. 8, pp. 1220-1220.View/Download from: Publisher's site
Elevated sea surface temperatures from a severe and prolonged El Niño event (2014-2016) fueled by climate change have resulted in mass coral bleaching (loss of dinoflagellate photosymbionts, Symbiodinium spp., from coral tissues) and subsequent coral mortality, devastating reefs worldwide. Genetic variation within and between Symbiodinium species strongly influences the bleaching tolerance of corals, thus recent papers have called for genetic engineering of Symbiodinium to elucidate the genetic basis of bleaching-relevant Symbiodinium traits. However, while Symbiodinium has been intensively studied for over 50 years, genetic transformation of Symbiodinium has seen little success likely due to the large evolutionary divergence between Symbiodinium and other model eukaryotes rendering standard transformation systems incompatible. Here, we integrate the growing wealth of Symbiodinium next-generation sequencing data to design tailored genetic engineering strategies. Specifically, we develop a testable expression construct model that incorporates endogenous Symbiodinium promoters, terminators, and genes of interest, as well as an internal ribosomal entry site from a Symbiodinium virus. Furthermore, we assess the potential for CRISPR/Cas9 genome editing through new analyses of the three currently available Symbiodinium genomes. Finally, we discuss how genetic engineering could be applied to enhance the stress tolerance of Symbiodinium, and in turn, coral reefs.
Murphy, CD, Ni, G, Li, G, Barnett, A, Xu, K, Grant-Burt, J, Liefer, JD, Suggett, DJ & Campbell, DA 2017, 'Quantitating active photosystem II reaction center content from fluorescence induction transients', Limnology and Oceanography: Methods, vol. 15, no. 1, pp. 54-69.View/Download from: Publisher's site
© 2016 The Authors Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. Photosystem II (PSII) is a pigment-protein complex that photochemically extracts electrons from water, generating the reductant that supports biological productivity in all biomes. Estimating the content of active PSII reaction centers in a liquid sample is a key input for estimating aquatic photosynthesis rates, as well as for analyzing phytoplankton stress responses. Established procedures for PSII content quantification based on oxygen evolution are slow, imprecise and require dense cell suspensions, and are thus inapplicable to many laboratory or field studies. A new approach uses baseline chlorophyll fluorescence emission divided by the effective absorbance cross section for PSII photochemistry, with both variables derivable from single turnover fluorescence induction protocols. This approach has not been widely tested and is potentially subject to variation in samples suffering progressive photoinactivation or induction of non-photochemical quenching under variable light. We evaluated the validity of this approach for a marine picocyanobacteria, low and high light Prochlorococcus ecotypes, arctic and temperate prasinophyte green alga and two centric diatoms, generating 209 paired determinations from a range of growth and treatment conditions. We successfully calibrated the fluorescence derived estimator for PSII reaction center content, and demonstrate a modification that corrects for the short term influence of photoinactivation. The modified parameter shows little response to induction of non-photochemical quenching. In doing so we show the potential and limitations of an estimator of active PSII reaction center content that is sufficiently robust to support rapid, time-resolved autonomous measures of primary productivity from lakes and oceans.
Suggett, DJ, Warner, ME & Leggat, W 2017, 'Symbiotic Dinoflagellate Functional Diversity Mediates Coral Survival under Ecological Crisis.', Trends in Ecology and Evolution, vol. 32, no. 10, pp. 735-745.View/Download from: Publisher's site
Coral reefs have entered an era of 'ecological crisis' as climate change drives catastrophic reef loss worldwide. Coral growth and stress susceptibility are regulated by their endosymbiotic dinoflagellates (genus Symbiodinium). The phylogenetic diversity of Symbiodinium frequently corresponds to patterns of coral health and survival, but knowledge of functional diversity is ultimately necessary to reconcile broader ecological success over space and time. We explore here functional traits underpinning the complex biology of Symbiodinium that spans free-living algae to coral endosymbionts. In doing so we propose a mechanistic framework integrating the primary traits of resource acquisition and utilisation as a means to explain Symbiodinium functional diversity and to resolve the role of Symbiodinium in driving the stability of coral reefs under an uncertain future.
Zhu, Y, Ishizaka, J, Tripathy, SC, Wang, S, Sukigara, C, Goes, J, Matsuno, T & Suggett, DJ 2017, 'Relationship between light, community composition and the electron requirement for carbon fixation in natural phytoplankton', Marine Ecology Progress Series, vol. 580, pp. 83-100.View/Download from: Publisher's site
© Inter-Research 2017. Fast repetition rate fluorometry (FRRF) provides a means to examine primary productivity at high resolution across broad scales, but must be coupled with independent knowledge of the electron requirement for carbon uptake (KC) to convert FRRF-measured electron transfer rate (ETR) to an inorganic carbon (C) uptake rate. Previous studies have demonstrated that variability of KC can be explained by key environmental factors (e.g. light, nutrients, t emperature). However, how such reconciliation of KC reflects changes of phytoplankton physiological status versus that of community composition has not been well resolved. Therefore, using a dataset of coupled FRRF and C uptake measurements, we examined how the environmental dependency of KC potentially varied with parallel changes in phytoplankton community structure. Data were combined from 14 campaigns conducted during the summer season throughout 2007 to 2014 in the East China Sea (ECS) and Tsushima Strait (TS). KC varied considerably, but this variability was best explained by a linear relationship with light availability (R2 = 0.66). Co-variability between KC and light availability was slightly improved by considering data as 2 clusters of physico-chemical conditions (R2 = 0.74), but was best improved as 2 taxonomic clusters: samples dominated by micro-phytoplankton ( > 20 μm) versus small phytoplankton (nano + pico, < 20 μm; R2 = 0.70-0.81). Interaction of phytoplankton community structure with light availability therefore explains the majority of variance of KC. The algorithms generated through our analysis therefore provide a means to examine C uptake with high resolution from future FRRF observations from these waters.
Szabó, M, Larkum, AWD, Suggett, DJ, Vass, I, Sass, L, Osmond, B, Zavafer, A, Ralph, PJ & Chow, WS 2017, 'Non-intrusive assessment of photosystem II and photosystem I in whole coral tissues', Frontiers in Marine Science, vol. 4, pp. 1-12.View/Download from: Publisher's site
© 2017 Szabó, Larkum, Suggett, Vass, Sass, Osmond, Zavafer, Ralph and Chow. Reef building corals (phylum Cnidaria) harbor endosymbiotic dinoflagellate algae (genus Symbiodinium) that generate photosynthetic products to fuel their host's metabolism. Non-invasive techniques such as chlorophyll (Chl) fluorescence analyses of Photosystem II (PSII) have been widely used to estimate the photosynthetic performance of Symbiodinium in hospite. However, since the spatial origin of PSII chlorophyll fluorescence in coral tissues is uncertain, such signals give limited information on depth-integrated photosynthetic performance of the whole tissue. In contrast, detection of absorbance changes in the near infrared (NIR) region integrates signals from deeper tissue layers due to weak absorption and multiple scattering of NIR light. While extensively utilized in higher plants, NIR bio-optical techniques are seldom applied to corals. We have developed a non-intrusive measurement method to examine photochemistry of intact corals, based on redox kinetics of the primary electron donor in Photosystem I (P700) and chlorophyll fluorescence kinetics (Fast-Repetition Rate fluorometry, FRRf). Since the redox state of P700 depends on the operation of both PSI and PSII, important information can be obtained on the PSII-PSI intersystem electron transfer kinetics. Under moderate, sub-lethal heat stress treatments (33 ◦ C for~20 min), the coral Pavona decussata exhibited down-regulation of PSII electron transfer kinetics, indicated by slower rates of electron transport from Q A to plastoquinone (PQ) pool, and smaller relative size of oxidized PQ with concomitant decrease of a specifically-defined P700 kinetics area, which represents the active pool of PSII. The maximum quantum efficiency of PSII (F v /F m ) and functional absorption cross-section of PSII (σ PSII ) remained unchanged. Based on the coordinated response of P700 parameters and PSII-PSI electron transport properties, we propose that...
Camp, EF, Dong, LF, Suggett, DJ, Smith, DJ, Boatman, TG, Crosswell, JR, Evenhuis, C, Scorfield, S, Walinjkar, A, Woods, J & Lawson, T 2017, 'A novel membrane inlet-infrared gas analysis (MI-IRGA) system for monitoring of seawater carbonate system', Limnology and Oceanography: Methods, vol. 15, no. 1, pp. 38-53.View/Download from: Publisher's site
© 2016 The Authors Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. Increased atmospheric CO 2 concentrations are driving changes in ocean chemistry at unprecedented rates resulting in ocean acidification, which is predicted to impact the functioning of marine biota, in particular of marine calcifiers. However, the precise understanding of such impacts relies on an analytical system that determines the mechanisms and impact of elevated pCO 2 on the physiology of organisms at scales from species to entire communities. Recent work has highlighted the need within experiments to control all aspects of the carbonate system to resolve the role of different inorganic carbon species on the physiological responses observed across taxa in real-time. Presently however, there are limited options available for continuous quantification of physiological responses, coupled with real-time calculation of the seawater carbonate chemistry system within microcosm environments. Here, we describe and characterise the performance of a novel pCO 2 membrane equilibrium system (the Membrane Inlet Infra-Red Gas Analyser, MI-IRGA) integrated with a continuous pH and oxygen monitoring platform. The system can detect changes in the seawater carbonate chemistry and determine organism physiological responses, while providing the user with real-time control over the microcosm system. We evaluate the systems control, response time and associated error, and demonstrate the flexibility of the system to operate under field conditions and within a laboratory. We use the system to measure physiological parameters (photosynthesis and respiration) for the corals Pocillipora damicornis and Porites cylindrica; in doing so we present a novel dataset examining the interactive role of temperature, light and pCO 2 on the physiology of P. cylindrica.
Camp, EF, Nitschke, MR, Rodolfo-Metalpa, R, Houlbreque, F, Gardner, SG, Smith, DJ, Zampighi, M & Suggett, DJ 2017, 'Reef-building corals thrive within hot-acidified and deoxygenated waters.', Scientific reports, vol. 7, no. 1, pp. 2434-2434.View/Download from: Publisher's site
Coral reefs are deteriorating under climate change as oceans continue to warm and acidify and thermal anomalies grow in frequency and intensity. In vitro experiments are widely used to forecast reef-building coral health into the future, but often fail to account for the complex ecological and biogeochemical interactions that govern reefs. Consequently, observations from coral communities under naturally occurring extremes have become central for improved predictions of future reef form and function. Here, we present a semi-enclosed lagoon system in New Caledonia characterised by diel fluctuations of hot-deoxygenated water coupled with tidally driven persistently low pH, relative to neighbouring reefs. Coral communities within the lagoon system exhibited high richness (number of species = 20) and cover (24-35% across lagoon sites). Calcification rates for key species (Acropora formosa, Acropora pulchra, Coelastrea aspera and Porites lutea) for populations from the lagoon were equivalent to, or reduced by ca. 30-40% compared to those from the reef. Enhanced coral respiration, alongside high particulate organic content of the lagoon sediment, suggests acclimatisation to this trio of temperature, oxygen and pH changes through heterotrophic plasticity. This semi-enclosed lagoon therefore provides a novel system to understand coral acclimatisation to complex climatic scenarios and may serve as a reservoir of coral populations already resistant to extreme environmental conditions.
Lohr, KE, Smith, DJ, Suggett, DJ, Nitschke, MR, Dumbrell, AJ, Woodcock, S & Camp, EF 2017, 'Coral Community Structure and Recruitment in Seagrass Meadows', Frontiers in Marine Science, vol. 4, pp. 1-13.View/Download from: Publisher's site
Goyen, S, Pernice, M, Szabó, M, Warner, ME, Ralph, PJ & Suggett, DJ 2017, 'A molecular physiology basis for functional diversity of hydrogen peroxide production amongst Symbiodinium spp. (Dinophyceae)', Marine Biology: international journal on life in oceans and coastal waters, vol. 164, no. 3.View/Download from: Publisher's site
Wangpraseurt, D, Holm, JB, Larkum, AWD, Pernice, M, Ralph, PJ, Suggett, DJ & Kühl, M 2017, 'In vivo Microscale Measurements of Light and Photosynthesis during Coral Bleaching: Evidence for the Optical Feedback Loop?', Frontiers in Microbiology, vol. 8, pp. 1-12.View/Download from: Publisher's site
Climate change-related coral bleaching, i.e., the visible loss of zooxanthellae from the coral host, is increasing in frequency and extent and presents a major threat to coral reefs globally. Coral bleaching has been proposed to involve accelerating light stress of their microalgal endosymbionts via a positive feedback loop of photodamage, symbiont expulsion and excess in vivo light exposure. To test this hypothesis, we used light and O2 microsensors to characterize in vivo light exposure and photosynthesis of Symbiodinium during a thermal stress experiment. We created tissue areas with different densities of Symbiodinium cells in order to understand the optical properties and light microenvironment of corals during bleaching. Our results showed that in bleached Pocillopora damicornis corals, Symbiodinium light exposure was up to fivefold enhanced relative to healthy corals, and the relationship between symbiont loss and light enhancement was well-described by a power-law function. Cell-specific rates of Symbiodinium gross photosynthesis and light respiration were enhanced in bleached P. damicornis compared to healthy corals, while areal rates of net photosynthesis decreased. Symbiodinium light exposure in Favites sp. revealed the presence of low light microniches in bleached coral tissues, suggesting that light scattering in thick coral tissues can enable photoprotection of cryptic symbionts. Our study provides evidence for the acceleration of in vivo light exposure during coral bleaching but this optical feedback mechanism differs between coral hosts. Enhanced photosynthesis in relation to accelerating light exposure shows that coral microscale optics exerts a key role on coral photophysiology and the subsequent degree of radiative stress during coral bleaching.
Hopkins, FE, Bell, TG, Yang, M, Suggett, DJ & Steinke, M 2016, 'Air exposure of coral is a significant source of dimethylsulfide (DMS) to the atmosphere', SCIENTIFIC REPORTS, vol. 6.View/Download from: Publisher's site
Zhu, Y, Ishizaka, J, Tripathy, SC, Wang, S, Mino, Y, Matsuno, T & Suggett, DJ 2016, 'Variation of the photosynthetic electron transfer rate and electron requirement for daily net carbon fixation in Ariake Bay, Japan', Journal of Oceanography, vol. 72, no. 5, pp. 761-776.View/Download from: Publisher's site
The Oceanographic Society of Japan and Springer Japan Fast repetition rate fluorometry (FRRf) provides a potential means to examine marine primary productivity; however, FRRf-based productivity estimations require knowledge of the electron requirement (K) for carbon (C) uptake (KC) to scale an electron transfer rate (ETR) to the CO2 uptake rate. Most previous studies have derived KC from parallel measurements of ETR and CO2 uptake over relatively short incubations, with few from longer-term daily-integrated periods. Here we determined KC by comparing depth-specific, daily ETRs and CO2-uptake rates obtained from 24-h on-deck incubation experiments undertaken on seven cruises in Ariake Bay, Japan, from 2008 to 2010. The purpose of this study was to determine the extent of variability of KC and to what extent this variability could be reconciled with the prevailing environmental conditions and ultimately to develop a method for determining net primary productivity (NPP) based on FRRf measurements. Both daily ETR and KC of the upper layer varied considerably, from 0.5 to 115.7 mmol e− mg Chl-a−1 day−1 and 4.1–26.6 mol e− (mol C)−1, respectively, throughout the entire data set. Multivariate analysis revealed a strong correlation between daily photosynthetically active radiation (PAR) and KC (r2 = 0.94). A simple PAR-dependent relationship derived from the data set was used for generating KC, and this relationship was validated by comparing the FRRf-predicted NPP with the 13C uptake measured in 2007. These new observations demonstrate the potential application of FRRf for estimating regional NPP from ETR.
Camp, EF, Smith, DJ, Evenhuis, C, Enochs, I, Manzello, D, Woodcock, S & Suggett, DJ 2016, 'Acclimatization to high-variance habitats does not enhance physiological tolerance of two key Caribbean corals to future temperature and pH', PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, vol. 283, no. 1831.View/Download from: Publisher's site
Camp, EF, Suggett, DJ, Gendron, G, Jompa, J, Manfrino, C & Smith, DJ 2016, 'Mangrove and Seagrass Beds Provide Different Biogeochemical Services for Corals Threatened by Climate Change', Frontiers in Marine Science, vol. 3, pp. 1-16.View/Download from: Publisher's site
Rapidly rising atmospheric CO2 concentrations are driving acidification in parallel with
warming of the oceans. Future ocean acidification scenarios have the potential to impact
coral growth and associated reef function, although reports suggest such affects could
be reduced in adjacent seagrass habitats as a result of physio-chemical buffering.
To-date, it remains unknown whether these habitats can actually support the metabolic
function of a diverse range of corals. Similarly, whether mangroves provide the same
ecological buffering service remains unclear. We examine whether reef-associated habitat
sites (seagrass and mangroves) can act as potential refugia to future climate change
by maintaining favorable chemical conditions (elevated pH and aragonite saturation
state relative to the open-ocean), but by also assessing whether the metabolic function
(photosynthesis, respiration and calcification) of important reef-building corals are
sustained. We investigated three sites in the Atlantic, Indian, and Pacific Oceans and
consistently observed that seagrass beds experience an overall elevation in mean pH
(8.15 ± 0.01) relative to the adjacent outer-reef (8.12 ± 0.03), but with periods of high
and low pH. Corals in the seagrass habitats either sustained calcification or experienced
an average reduction of 17.0 ± 6.1% relative to the outer-reef. In contrast, mangrove
habitats were characterized by a low mean pH (8.04 ± 0.01) and a relatively moderate
pH range. Corals within mangrove-dominated habitats were thus pre-conditioned to low
pH but with significant suppression to calcification (70.0 ± 7.3% reduction relative to the
outer-reef). Both habitats also experienced more variable temperatures (diel range up to
2.5◦C) relative to the outer-reef (diel range less than 0.7◦C), which did not correspond with
changes in calcification rates. Here we report, for the first time, the biological costs for
corals living in reef-associated habitats and characterize the environme...
Murray, SA, Suggett, DJ, Seymour, JR, Doblin, M, Kohli, GS, Fabris, M & Ralph, PJ 2016, 'Unravelling the functional genetics of dinoflagellates: a review of approaches and opportunities', Perspectives in Phycology, vol. 3, no. 1, pp. 37-52.View/Download from: Publisher's site
Dinoflagellates occupy an extraordinarily diverse array of ecological niches. Their success stems from a suite of functional and ecological strategies, including the production of secondary metabolites with anti-predator or allelopathic impacts, nutritional flexibility, and the ability to form symbiotic relationships. Despite their ecological importance, we currently have a poor understanding of the genetic basis for many of these strategies, due to the complex genomes of dinoflagellates. Genomics and transcriptomic sequencing approaches are now providing the first insights into the genetic basis of some dinoflagellate functional traits, providing the opportunity for novel ecological experiments, novel methods for monitoring of harmful biotoxins, and allowing us to investigate the production of ecologically and economically important compounds such as the long chain polyunsaturated fatty acid, docosahexanoic acid and the climatically important metabolite, dimethylsulfoniopropionate. Despite these advances, we still generally lack the ability to genetically manipulate species, which would enable the confirmation of biosynthetic pathways and the development of novel bio-engineering applications. Here, we describe advances in understanding the genetic basis of dinoflagellate ecology, and propose biotechnological approaches that could be applied to further transform our understanding of this unique group of eukaryotes.
Barnes, MK, Tilstone, GH, Smyth, TJ, Widdicombe, CE, Gloël, J, Robinson, C, Kaiser, J & Suggett, DJ 2015, 'Drivers and effects of Karenia mikimotoi blooms in the western English Channel', Progress in Oceanography, vol. 137, no. B, pp. 456-469.View/Download from: Publisher's site
© 2015. Naturally occurring red tides and harmful algal blooms (HABs) are of increasing importance in the coastal environment and can have dramatic effects on coastal benthic and epipelagic communities worldwide. Such blooms are often unpredictable, irregular or of short duration, and thus determining the underlying driving factors is problematic. The dinoflagellate Karenia mikimotoi is an HAB, commonly found in the western English Channel and thought to be responsible for occasional mass finfish and benthic mortalities. We analysed a 19-year coastal time series of phytoplankton biomass to examine the seasonality and interannual variability of K. mikimotoi in the western English Channel and determine both the primary environmental drivers of these blooms as well as the effects on phytoplankton productivity and oxygen conditions. We observed high variability in timing and magnitude of K. mikimotoi blooms, with abundances reaching >1000cellsmL-1 at 10m depth, inducing up to a 12-fold increase in the phytoplankton carbon content of the water column. No long-term trends in the timing or magnitude of K. mikimotoi abundance were evident from the data. Key driving factors were identified as persistent summertime rainfall and the resultant input of low-salinity high-nutrient river water. The largest bloom in 2009 was associated with highest annual primary production and led to considerable oxygen depletion at depth, most likely as a result of enhanced biological breakdown of bloom material; however, this oxygen depletion may not affect zooplankton. Our data suggests that K. mikimotoi blooms are not only a key and consistent feature of western English Channel productivity, but importantly can potentially be predicted from knowledge of rainfall or river discharge.
Barnes, MK, Tilstone, GH, Suggett, DJ, Widdicombe, CE, Bruun, J, Martinez-Vicente, V & Smyth, TJ 2015, 'Temporal variability in total, micro- and nano-phytoplankton primary production at a coastal site in the Western English Channel', Progress in Oceanography, vol. 137, no. B, pp. 470-483.View/Download from: Publisher's site
© 2015. Primary productivity and subsequent carbon cycling in the coastal zone have a significant impact on the global carbon budget. It is currently unclear how anthropogenic activity could alter these budgets but long term coastal time series of hydrological, biogeochemical and biological measurements represent a key means to better understand past drivers, and hence to predicting future seasonal and inter-annual variability in carbon fixation in coastal ecosystems. An 8-year time series of primary production from 2003 to 2010, estimated using a recently developed absorption-based algorithm, was used to determine the nature and extent of change in primary production at a coastal station (L4) in the Western English Channel (WEC). Analysis of the seasonal and inter-annual variability in production demonstrated that on average, nano- and pico-phytoplankton account for 48% of the total carbon fixation and micro-phytoplankton for 52%. A recent decline in the primary production of nano- and pico-phytoplankton from 2005 to 2010 was observed, corresponding with a decrease in winter nutrient concentrations and a decrease in the biomass of Phaeocystis sp. Micro-phytoplankton primary production (PPM) remained relatively constant over the time series and was enhanced in summer during periods of high precipitation. Increases in sea surface temperature, and decreases in wind speeds and salinity were associated with later spring maxima in PPM. Together these trends indicate that predicted increases in temperature and decrease in wind speeds in future would drive later spring production whilst predicted increases in precipitation would also continue these blooms throughout the summer at this site.
Exton, DA, McGenity, TJ, Steinke, M, Smith, DJ & Suggett, DJ 2015, 'Uncovering the volatile nature of tropical coastal marine ecosystems in a changing world.', Global change biology, vol. 21, no. 4, pp. 1383-1394.View/Download from: Publisher's site
Biogenic volatile organic compounds (BVOCs), in particular dimethyl sulphide (DMS) and isoprene, have fundamental ecological, physiological and climatic roles. Our current understanding of these roles is almost exclusively established from terrestrial or oceanic environments but signifies a potentially major, but largely unknown, role for BVOCs in tropical coastal marine ecosystems. The tropical coast is a transition zone between the land and ocean, characterized by highly productive and biodiverse coral reefs, seagrass beds and mangroves, which house primary producers that are amongst the greatest emitters of BVOCs on the planet. Here, we synthesize our existing understanding of BVOC emissions to produce a novel conceptual framework of the tropical marine coast as a continuum from DMS-dominated reef producers to isoprene-dominated mangroves. We use existing and previously unpublished data to consider how current environmental conditions shape BVOC production across the tropical coastal continuum, and in turn how BVOCs can regulate environmental stress tolerance or species interactions via infochemical networks. We use this as a framework to discuss how existing predictions of future tropical coastal BVOC emissions, and the roles they play, are effectively restricted to present day 'baseline' trends of BVOC production across species and environmental conditions; as such, there remains a critical need to focus research efforts on BVOC responses to rapidly accelerating anthropogenic impacts at local and regional scales. We highlight the complete lack of current knowledge required to understand the future ecological functioning of these important systems, and to predict whether feedback mechanisms are likely to regulate or exacerbate current climate change scenarios through environmentally and ecologically mediated changes to BVOC budgets at the ecosystem level.
Frommlet, JC, Sousa, ML, Alves, A, Vieira, SI, Suggett, DJ & Serodio, J 2015, 'Coral symbiotic algae calcify ex hospite in partnership with bacteria', PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 112, no. 19, pp. 6158-6163.View/Download from: Publisher's site
Silsbe, GM, Oxborough, K, Suggett, DJ, Forster, RM, Ihnken, S, Komarek, O, Lawrenz, E, Prasil, O, Rottgers, R, Sicner, M, Simis, SGH, Van Dijk, MA & Kromkamp, JC 2015, 'Toward autonomous measurements of photosynthetic electron transport rates: An evaluation of active fluorescence-based measurements of photochemistry', Limnology and Oceanography: Methods, vol. 13, no. 3, pp. 138-155.View/Download from: Publisher's site
Suggett, DJ, Goyen, S, Evenhuis, C, Szabo, M, Pettay, DT, Warner, ME & Ralph, PJ 2015, 'Functional diversity of photobiological traits within the genus Symbiodinium appears to be governed by the interaction of cell size with cladal designation', New Phytologist, vol. 208, no. 2, pp. 370-381.View/Download from: Publisher's site
© 2015 New Phytologist Trust. Dinoflagellates of the genus Symbiodinium express broad diversity in both genetic identity (phylogeny) and photosynthetic function to presumably optimize ecological success across extreme light environments; however, whether differences in the primary photobiological characteristics that govern photosynthetic optimization are ultimately a function of phylogeny is entirely unresolved. We applied a novel fast repetition rate fluorometry approach to screen genetically distinct Symbiodinium types (n = 18) spanning five clades (A-D, F) for potential phylogenetic trends in factors modulating light absorption (effective cross-section, reaction center content) and utilization (photochemical vs dynamic nonphotochemical quenching; [1 - C] vs [1 - Q]) by photosystem II (PSII). The variability of PSII light absorption was independent of phylogenetic designation, but closely correlated with cell size across types, whereas PSII light utilization intriguingly followed one of three characteristic patterns: (1) similar reliance on [1 - C] and [1 - Q] or (2) preferential reliance on [1 - C] (mostly A, B types) vs (3) preferential reliance on [1 - Q] (mostly C, D, F types), and thus generally consistent with cladal designation. Our functional trait-based approach shows, for the first time, how Symbiodinium photosynthetic function is governed by the interplay between phylogenetically dependent and independent traits, and is potentially a means to reconcile complex biogeographic patterns of Symbiodinium phylogenetic diversity in nature.
Tamburic, B, Evenhuis, CR, Suggett, DJ, Larkum, AWD, Raven, JA & Ralph, PJ 2015, 'Gas Transfer Controls Carbon Limitation During Biomass Production by Marine Microalgae', CHEMSUSCHEM, vol. 8, no. 16, pp. 2727-2736.View/Download from: Publisher's site
Camp, EF, Krause, SL, Santos, LMF, Naumann, MS, Kikuchi, RKP, Smith, DJ, Wild, C & Suggett, DJ 2015, 'The "Flexi-Chamber": A Novel Cost-Effective In Situ Respirometry Chamber for Coral Physiological Measurements', PLoS One, vol. 10, no. 10, pp. 1-21.View/Download from: Publisher's site
Barnes, MK, Tilstone, GH, Smyth, TJ, Suggett, DJ, Astoreca, R, Lancelot, C & Kromkamp, JC 2014, 'Absorption-based algorithm of primary production for total and size-fractionated phytoplankton in coastal waters', Marine Ecology Progress Series, vol. 504, pp. 73-89.View/Download from: Publisher's site
Most satellite models of production have been designed and calibrated for use in the open ocean. Coastal waters are optically more complex, and the use of chlorophyll a (chl a) as a first-order predictor of primary production may lead to substantial errors due to significant quantities of coloured dissolved organic matter (CDOM) and total suspended material (TSM) within the first optical depth. We demonstrate the use of phytoplankton absorption as a proxy to estimate primary production in the coastal waters of the North Sea and Western English Channel for both total, micro- and nano+pico-phytoplankton production. The method is implemented to extrapolate the absorption coefficient of phytoplankton and production at the sea surface to depth to give integrated fields of total and micro- and nano+pico-phytoplankton primary production using the peak in absorption coefficient at red wavelengths. The model is accurate to 8% in the Western English Channel and 22% in this region and the North Sea. By comparison, the accuracy of similar chl a based production models was >250%. The applicability of the method to autonomous optical sensors and remotely sensed aircraft data in both coastal and estuarine environments is discussed.
Brodie, J, Williamson, CJ, Smale, D, Kamenos, KA, Mieszkowska, N, Santos, R, Cunliffe, M, Steinke, M, Yesson, C, Anderson, KM, Asnaghi, V, Brownlee C, Burdett, HL, Burrows, MT, Collins, S, Donahue, PJC, Harvey, B, Foggo, A, Noisette, F, Nunes, J, Ragazzola, F, Raven, JA, Schmidt, DN, Suggett, DJ, Teichberg, M & Hall-Spencer, JM 2014, 'The future of the northeast Atlantic benthic flora in a high CO2 world', Ecology & Evolution, vol. 4, no. 13, pp. 2787-2798.View/Download from: Publisher's site
Seaweed and seagrass communities in the northeast Atlantic have been profoundly impacted by humans, and the rate of change is accelerating rapidly due to runaway CO2 emissions and mounting pressures on coastlines associated with human population growth and increased consumption of finite resources. Here, we predict how rapid warming and acidification are likely to affect benthic flora and coastal ecosystems of the northeast Atlantic in this century, based on global evidence from the literature as interpreted by the collective knowledge of the authorship. We predict that warming will kill off kelp forests in the south and that ocean acidification will remove maerl habitat in the north. Seagrasses will proliferate, and associated epiphytes switch from calcified algae to diatoms and filamentous species. Invasive species will thrive in niches liberated by loss of native species and spread via exponential development of artificial marine structures. Combined impacts of seawater warming, ocean acidification, and increased storminess may replace structurally diverse seaweed canopies, with associated calcified and noncalcified flora, with simple habitats dominated by noncalcified, turf-forming seaweeds.
Poulton, AJ, Stinchcombe, MC, Achterberg, EP, Bakker, DCE, Dumousseaud, C, Lawson, HE, Lee, GA, Richier, S, Suggett, DJ & Young, JR 2014, 'Coccolithophores on the north-west European shelf: calcification rates and environmental controls', BIOGEOSCIENCES, vol. 11, no. 14, pp. 3919-3940.View/Download from: Publisher's site
Richier, S, Achterberg, EP, Dumousseaud, C, Poulton, AJ, Suggett, DJ, Tyrrell, T, Zubkov, MV & Moore, CM 2014, 'Phytoplankton responses and associated carbon cycling during shipboard carbonate chemistry manipulation experiments conducted around Northwest European shelf seas', BIOGEOSCIENCES, vol. 11, no. 17, pp. 4733-4752.View/Download from: Publisher's site
Wu, Y, Campbell, DA, Irwin, AJ, Suggett, DJ & Finkel, Z 2014, 'Ocean acidification enhances the growth rate of larger diatoms', Limnology and Oceanography, vol. 59, no. 3, pp. 1027-1034.View/Download from: Publisher's site
Ocean acidification is changing the nature of inorganic carbon availability in the global oceans. Diatoms account for ~ 40% of all marine primary productivity and are major contributors to the export of atmospheric carbon to the deep ocean. Larger diatoms are more likely to be stimulated by future increases in CO2 availability as a result of their low surface area to volume ratio and lower diffusive flux of CO2 relative to their carbon demand for growth. Here we quantify the effect of the partial pressure of carbon dioxide (), at levels of 190, 380, and 750 µL L-1, on the growth rate, photosystem II electron transport rate (ETR), and elemental composition for five diatom species ranging over five orders of magnitude in cell volume. Growth rates for all species were enhanced under 750 relative to 190 and 380 µL L-1, with little change in ETR or elemental stoichiometries, indicating an enhanced allocation of photochemical energy to growth under elevated . enhancement of growth rates was size dependent. Under 750 vs. 190 µL L-1 partial pressures, growth rate was enhanced by ~ 5% for the smaller diatom species to ~ 30% for the largest species examined. The size dependence of CO2-stimulated growth enhancement indicates that ocean acidification may selectively favor an increase in the growth rates of larger vs. smaller phytoplankton species in the sea, with potentially significant consequences for carbon biochemistry.
Wu, Y, Jeans, J, Suggett, DJ, Finkel, ZV & Campbell, DA 2014, 'Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates', Frontiers in Marine Science, vol. 1, pp. 1-11.View/Download from: Publisher's site
© 2014 Wu, Jeans, Suggett, Finkel and Campbell. Diatoms contribute ~40% of primary production in the modern ocean and encompass the largest cell size range of any phytoplankton group. Diatom cell size influences their nutrient uptake, photosynthetic light capture, carbon export efficiency, and growth responses to increasing pCO 2 . We therefore examined nitrogen resource allocations to the key protein complexes mediating photosynthesis across six marine centric diatoms, spanning 5 orders of magnitude in cell volume, under past, current and predicted future pCO 2 levels, in balanced growth under nitrogen repletion. Membrane bound photosynthetic protein concentrations declined with cell volume in parallel with cellular concentrations of total protein, total nitrogen and chlorophyll. Larger diatom species, however, allocated a greater fraction (by 3.5-fold) of their total cellular nitrogen to the soluble Ribulose-1,5-bisphosphate Carboxylase Oxygenase (RUBISCO) carbon fixation complex than did smaller species. Carbon assimilation per unit of RUBISCO large subunit (C RbcL -1 s -1 ) decreased with cell volume, from ~8 to ~2 C RbcL -1 s -1 from the smallest to the largest cells. Whilst a higher allocation of cellular nitrogen to RUBISCO in larger cells increases the burden upon their nitrogen metabolism, the higher RUBISCO allocation buffers their lower achieved RUBISCO turnover rate to enable larger diatoms to maintain carbon assimilation rates per total protein comparable to small diatoms. Individual species responded to increased pCO 2 , but cell size effects outweigh pCO 2 responses across the diatom species size range examined. In large diatoms a higher nitrogen cost for RUBISCO exacerbates the higher nitrogen requirements associated with light absorption, so the metabolic cost to maintain photosynthesis is a cell size-dependent trait.
Robinson, C, Suggett, DJ, Cherukuru, N, Ralph, PJ & Doblin, MA 2014, 'Performance of Fast Repetition Rate fluorometry based estimates of primary productivity in coastal waters', JOURNAL OF MARINE SYSTEMS, vol. 139, pp. 299-310.View/Download from: Publisher's site
Szabo, M, Wangpraseurt, D, Tamburic, B, Larkum, A, Schreiber, U, Suggett, DJ, Kühl, M & Ralph, PJ 2014, 'Effective light absorption and absolute electron transport rates in the coral Pocillopora damicornis', Plant Physiology and Biochemistry, vol. 83, pp. 159-167.View/Download from: Publisher's site
Pulse Amplitude Modulation (PAM) fluorometry has been widely used to estimate the relative photosynthetic efficiency of corals. However, both the optical properties of intact corals as well as past technical constrains to PAM fluorometers have prevented calculations of the electron turnover rate of PSII. We used a new Multi-colour PAM (MC-PAM) in parallel with light microsensors to determine for the first time the wavelength-specific effective absorption cross-section of PSII photochemistry, sII(?), and thus PAM-based absolute electron transport rates of the coral photosymbiont Symbiodinium both in culture and in hospite in the coral Pocillopora damicornis. In both cases, sII of Symbiodinium was highest in the blue spectral region and showed a progressive decrease towards red wavelengths. Absolute values for sII at 440 nm were up to 1.5-times higher in culture than in hospite. Scalar irradiance within the living coral tissue was reduced by 20% in the blue when compared to the incident downwelling irradiance. Absolute electron transport rates of P. damicornis at 440 nm revealed a maximum PSII turnover rate of ca. 250 electrons PSII-1 s-1, consistent with one PSII turnover for every 4 photons absorbed by PSII; this likely reflects the limiting steps in electron transfer between PSII and PSI. Our results show that optical properties of the coral host strongly affect light use efficiency of Symbiodinium. Therefore, relative electron transport rates do not reflect the productivity rates (or indeed how the photosynthesis-light response is parameterised). Here we provide a non-invasive approach to estimate absolute electron transport rates in corals.
Tamburic, B, Szabo, M, Tran, A, Larkum, A, Suggett, DJ & Ralph, PJ 2014, 'Action spectra of oxygen production and chlorophyll a fluorescence in the green microalga Nannochloropsis oculata', Bioresource Technology, vol. 169, pp. 320-327.View/Download from: Publisher's site
The first complete action spectrum of oxygen evolution and chlorophyll a fluorescence was measured for the biofuel candidate alga Nannochloropsis oculata. A novel analytical procedure was used to generate a representative and reproducible action spectrum for microalgal cultures. The action spectrum was measured at 14 discrete wavelengths across the visible spectrum, at an equivalent photon flux density of 60 µmol photons m-2 s-1. Blue light (~414 nm) was absorbed more efficiently and directed to photosystem II more effectively than red light (~679 nm) at light intensities below the photosaturation limit. Conversion of absorbed photons into photosynthetic oxygen evolution was maximised at 625 nm; however, this maximum is unstable since neighbouring wavelengths (646 nm) resulted in the lowest photosystem II operating efficiency. Identifying the wavelength-dependence of photosynthesis has clear implications to optimising growth efficiency and hence important economic implications to the algal biofuels and bioproducts industries.
Wangpraseurt, D, Tamburic, B, Szabo, M, Suggett, DJ, Ralph, PJ & Kuhl, M 2014, 'Spectral Effects on Symbiodinium Photobiology Studied with a Programmable Light Engine', PLoS One.View/Download from: Publisher's site
The spectral light field of Symbiodinium within the tissue of the coral animal host can deviate strongly from the ambient light field on a coral reef and that of artificial light sources used in lab studies on coral photobiology. Here, we used a novel approach involving light microsensor measurements and a programmable light engine to reconstruct the spectral light field that Symbiodinium is exposed to inside the coral host and the light field of a conventional halogen lamp in a comparative study of Symbiodinium photobiology. We found that extracellular gross photosynthetic O2 evolution was unchanged under different spectral illumination, while the more red-weighted halogen lamp spectrum decreased PSII electron transport rates and there was a trend towards increased light-enhanced dark respiration rates under excess irradiance. The approach provided here allows for reconstructing and comparing intra-tissue coral light fields and other complex spectral compositions of incident irradiance. This novel combination of sensor technologies provides a framework to studying the influence of macro- and microscale optics on Symbiodinium photobiology with unprecedented spectral resolution.
Brading, P, Warner, ME, Smith, DJ & Suggett, DJ 2013, 'Contrasting modes of inorganic carbon acquisition amongst Symbiodinium (Dinophyceae) phylotypes', New Phytologist, vol. 200, no. 2, pp. 432-442.View/Download from: Publisher's site
Growing concerns over ocean acidification have highlighted the need to critically understand inorganic carbon acquisition and utilization in marine microalgae. Here, we contrast these characteristics for the first time between two genetically distinct di
Exton, DA, Suggett, DJ, McGenity, TJ & Steinke, M 2013, 'Chlorophyll-normalized isoprene production in laboratory cultures of marine microalgae and implications for global models', Limnology and Oceanography, vol. 58, no. 4, pp. 1301-1311.View/Download from: Publisher's site
We used laboratory cultures of marine microalgae to investigate the effects of growth conditions and their taxonomic position on the production of isoprene, a gas that has major effects on atmospheric chemistry and provides stress tolerance to many primary producers. Isoprene was quantified from 21 microalgal strains sampled during exponential growth, using purge-and-trap pre-concentration and gas chromatography with flameionization detection. Isoprene production rates varied by two orders of magnitude between strains (0.03 1.34 mmol [g chlorophyll a]21 h21), and were positively correlated with temperature (r2 5 0.52, p , 0.001, n 5 59). Three distinct sea surface temperature (SST)dependent relationships were found between isoprene and chlorophyll a (mmol [g chlorophyll a]21 h21), an improvement in resolution over the single relationship used in previous models: for three polar strains grown at 21uC (slope 5 0.03, R2 5 0.76, p , 0.05, n 5 9), nine strains grown at 16uC (slope 5 0.24, R2 5 0.43, p , 0.05, n 5 27 with Dunaliella tertiolecta excluded), and eight strains grown at 26uC (slope 5 0.39, R2 5 0.15, p , 0.05, n 5 24). We then used a simple model that applied the SSTdependent nature of isoprene production to three representative bioregions for the growth temperatures used in this study. This approach yielded an estimate of global marine isoprene production that was 51% higher than previous attempts using an SST-independent single relationship. Taking into account the effect of temperature therefore potentially allows more precise modeling of marine isoprene production, and suggests that increasing the SST-based resolution of data beyond the three groups used here could further improve future modeling simulations.
Krug, LA, Gherardi, DF, Stech, JL, Leão, ZM, Kikuchi, RK, Junior, ER & Suggett, DJ 2013, 'The construction of causal networks to estimate coral bleaching intensity', Environmental Modelling & Software, vol. 42, no. 1, pp. 157-167.View/Download from: Publisher's site
Current metrics for predicting bleaching episodes, e.g. NOAA's Coral Reef Watch Program, do not seem to apply well to Brazil's marginal reefs located in Bahia state and alternative predictive approaches must be sought for effective long term management.
Lawrenz, E, Silsbe, G, Capuzzo, E, Ylostalo, P, Forster, RM, Simis, SG, Prasil, O, Kromkamp, JC, Hickman, AE, Moore, CM, Forget, M, Geider, RJ & Suggett, DJ 2013, 'Predicting the electron requirement for carbon fixation in seas and oceans', Plos One, vol. 8, no. 3, pp. 0-0.View/Download from: Publisher's site
Marine phytoplankton account for about 50% of all global net primary productivity (NPP). Active fluorometry, mainly Fast Repetition Rate fluorometry (FRRf), has been advocated as means of providing high resolution estimates of NPP. However, not measuring
McGinley, MP, Suggett, DJ & Warner, ME 2013, 'Transcript patterns of chloroplast-encoded genes in cultured Symbiodinium spp. (Dinophyceae): testing the influence of a light shift and diel periodicity', Journal of Phycology, vol. 49, no. 4, pp. 709-718.View/Download from: Publisher's site
Microalgae possess numerous cellular mechanisms specifically employed for acclimating the photosynthetic pathways to changes in the physical environment. Despite the importance of coral-dinoflagellate symbioses, little focus has been given as to how the symbiotic algae (Symbiodinium spp.) regulate the expression of their photosynthetic genes. This study used real-time PCR to investigate the transcript abundance of the plastid-encoded genes, psbA (encoding the D1 protein of photosystem II) and psaA (encoding the P700 protein in photosystem I), within the cultured Symbiodinium ITS-2 (internal transcribed spacer region) types A20 and A13. Transcript abundance was monitored during a low to high-light shift, as well as over a full diel light cycle. In addition, psaA was characterized in three isolates (A20, A13, and D4-5) and noted as another example of a dinoflagellate plastid gene encoded on a minicircle. In general, the overall incongruence of transcript patterns for both psbA and psaA between the Symbiodinium isolates and other models of transcriptionally controlled chloroplast gene expression (e.g., Pisum sativum [pea], Sinapis alba [mustard seedling], and Synechocystis sp. PCC 6803 [cyanobacteria]) suggests that Symbiodinium is reliant on posttranscriptional mechanisms for homeostatic regulation of its photosynthetic proteins.
Suggett, DJ, Dong, LF, Lawson, T, Lawrenz, E, Torres, L & Smith, DJ 2013, 'Light availability determines susceptibility of reef building corals to ocean acidification', Coral Reefs, vol. 32, no. 2, pp. 327-337.View/Download from: Publisher's site
Elevated seawater pCO(2), and in turn ocean acidification (OA), is now widely acknowledged to reduce calcification and growth of reef building corals. As with other environmental factors (e.g., temperature and nutrients), light availability fundamentally
Ye, Z, Robakowski, P & Suggett, DJ 2013, 'A mechanistic model for the light response of photosynthetic electron transport rate based on light harvesting properties of photosynthetic pigment molecules', Planta, vol. 237, no. 3, pp. 837-847.View/Download from: Publisher's site
Models describing the light response of photosynthetic electron transport rate (ETR) are routinely used to determine how light absorption influences energy, reducing power and yields of primary productivity; however, no single model is currently able to
Ye, Z, Suggett, DJ, Robakowski, P & Kang, H 2013, 'A mechanistic model for the photosynthesis-light response based on the photosynthetic electron transport of photosystem II in C3 and C4 species', New Phytologist, vol. 199, no. 1, pp. 110-120.View/Download from: Publisher's site
A new mechanistic model of the photosynthesis-light response is developed based on photosynthetic electron transport via photosystem II (PSII) to specifically describe light-harvesting characteristics and associated biophysical parameters of photosynthet
Brading, P, Warner, ME, Davey, P, Smith, DJ, Achterberg, EP & Suggett, DJ 2012, 'Erratum: Differential effects of ocean acidification on growth and photosynthesis among phylotypes ofSymbiodinium(Dinophyceae)', Limnology and Oceanography, vol. 57, no. 4, pp. 1255-1255.View/Download from: Publisher's site
Clifton, J, Etienne, M, Barnes, DK, Barnes, RS, Suggett, DJ & Smith, DJ 2012, 'Marine conservation policy in Seychelles: Current constraints and prospects for improvement', Marine Policy, vol. 36, no. 3, pp. 823-831.View/Download from: Publisher's site
The marine assets of Seychelles are fundamental to the national economy through generating income derived from tourism and fisheries. Marine management institutions and policies have undergone radical changes since 2008, reflecting a number of drivers an
Exton, DA, Suggett, DJ, Steinke, M & McGenity, TJ 2012, 'Spatial and temporal variability of biogenic isoprene emissions from a temperate estuary', Global Biogeochemical Cycles, vol. 26, no. 2, pp. 1-13.View/Download from: Publisher's site
Isoprene is important for its atmospheric impacts and the ecophysiological benefits it affords to emitting organisms; however, isoprene emissions from marine systems remain vastly understudied compared to terrestrial systems. This study investigates for the first time drivers of isoprene production in a temperate estuary, and the role this production may play in enabling organisms to tolerate the inherently wide range of environmental conditions. Intertidal sediment cores as well as high and low tide water samples were collected from four sites along the Colne Estuary, UK, every six weeks over a year. Isoprene concentrations in the water were significantly higher at low than high tide, and decreased toward the mouth of the estuary; sediment production showed no spatial variability. Diel isoprene concentration increased with light availability and decreased with tidal height; nighttime production was 79% lower than daytime production. Seasonal isoprene production and water concentrations were highest for the warmest months, with production strongly correlated with light (r2 = 0.800) and temperature (r2 = 0.752). Intertidal microphytobenthic communities were found to be the primary source of isoprene, with tidal action acting as a concentrating factor for isoprene entering the water column. Using these data we estimated an annual production rate for this estuary of 681 mmol m2 y1 . This value falls at the upper end of other marine estimates and highlights the potentially significant role of estuaries as isoprene sources. The control of estuarine isoprene production by environmental processes identified here further suggests that such emissions may be altered by future environmental change.
Green, B, Suggett, DJ, Hills, AJ & Steinke, M 2012, 'Optimisation of a fast DMS sensor (FDS) for real time quantification of dimethyl sulfide production by algae', Biogeochemistry, vol. 110, no. 1-3, pp. 163-172.View/Download from: Publisher's site
Production of dimethyl sulfide (DMS) from marine samples is often quantified using gas chromatography techniques. Typically, these are labour intensive and have a slow sample turnover rate. Here we demonstrate the use of a portable fast DMS sensor (FDS) that utilises the chemiluminescent reaction of DMS and ozone to measure DMS production in aqueous samples, with a maximum frequency of 10 Hz. We have developed a protocol for quantifying DMS production that removes potential signal interference from other biogenic trace gases such as isoprene (2-methyl-1,3-butadiene) and hydrogen sulfide. The detection limit was 0.89 pM (0.02 ppbv) when using a DMS standard gas mixture. The lowest DMS production rates quantified with the FDS and verified using conventional gas chromatography with flame photometric detection (GC-FPD) were around 0.01 nmol min-1. There was a strong correlation in DMS production when comparing the FDS and GC-FPD techniques with a range of marine samples (e.g., r2 = 0.94 for Emiliania huxleyi). However, the combined dataset showed the FDS measured 22% higher DMS production than the GC-FPD, with the differences in rates likely due to interfering gases, for example hydrogen sulfide and isoprene. This possible overestimation of DMS production is smaller than the two-fold difference in DMS production between day and night samples from a culture of E. huxleyi. The response time of the instrument to changes in DMS production is method dependent (e.g., geometry of incubation vessel, bubble size) and was approximately 4 min under our conditions when using a culture of E.huxleyi (800 ml) with aeration at 100 ml min-1. We suggest the FDS can reduce sample handling, is suitable for short- and long-term measurements of DMS production in algal cultures, and will widen the range of DMS research in marine environments.
Mansell, PJ, Suggett, DJ & Smith, DJ 2012, 'Management of marine resources; combining industry and research FOREWORD', JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM, vol. 92, no. 4, pp. 643-646.View/Download from: Publisher's site
Oxborough, K, Moore, CM, Suggett, DJ, Lawson, T, Chan, HG & Geider, RJ 2012, 'Direct estimation of functional PSII reaction center concentration and PSII electron flux on a volume basis: a new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data', Limnology and Oceanography: Methods, vol. 10, pp. 142-154.View/Download from: Publisher's site
Smale, D, Barnes, D, Barnes, R, Smith, D & Suggett, DJ 2012, 'Spatial Variability In The Structure Of Intertidal Crab And Gastropod Assemblages Within The Seychelles Archipelago (indian Ocean)', Journal of Sea Research, vol. 69, pp. 8-15.View/Download from: Publisher's site
Tropical nearshore ecosystems represent global hotspots of marine biodiversity and endemism but are often poorly understood and impacted by human activities. The Seychelles Archipelago (Western Indian Ocean) sustains a wealth of marine life, much of which is threatened by rapid development associated with tourism and climate change. Six marine parks exist within the Archipelago, but their biodiversity value and ecological health are poorly known, especially with regards to non-fish and coral species. Here we investigate spatial patterns of littoral biodiversity on 6 islands, 5 of which were granitic and within marine parks, including the first surveys of Curieuse and Ile Cocos. Our surveys formed a nested sampling design, to facilitate an examination of variability in species richness, faunal abundance, taxonomic distinctness and assemblage composition at multiple spatial scales, from islands (>100 s km) to quadrats (metres). We identified (mostly to species) and enumerated two target taxa, brachyuran decapod crustaceans and gastropod molluscs, and recorded over 8300 individuals belonging to over 150 species. Crabs and gastropods exhibited different patterns of spatial variability, as crab assemblages were generally more distinct between islands, while gastropod assemblages were markedly variable at the smallest spatial scales of `patch and `quadrat. Intertidal biodiversity was greatest on Curieuse Island and least at Desroches, the latter was being the only coral atoll we surveyed and thereby differing in its geological and ecological context. We discuss likely drivers of these biodiversity patterns and highlight urgently-needed research directions. Our assessment of the status of poorly-known invertebrate assemblages across the Seychelles will complement more extensive surveys of coral and fish assemblages and, in doing so, provide a useful baseline for monitoring the effects of key stressors in the region, such as coastal development and climae change.
Suggett, DJ, Hall-Spencer, JM, Rodolfo-Metalpa, R, Boatman, TG, Payton, R, Pettay, DT, Johnson, VR, Warner, ME & Lawson, T 2012, 'Sea anemones may thrive in a high CO2 world', Global Change Biology, vol. 18, no. 10, pp. 3015-3025.View/Download from: Publisher's site
Increased seawater pCO(2), and in turn ocean acidification (OA), is predicted to profoundly impact marine ecosystem diversity and function this century. Much research has already focussed on calcifying reef-forming corals (Class: Anthozoa) that appear pa
Suggett, DJ, Kikuchi, RK, Oliveira, MD, Spano, S, Carvalho, R & Smith, DJ 2012, 'Photobiology of corals from Brazil's near-shore marginal reefs of Abrolhos', Marine Biology, vol. 159, no. 7, pp. 1461-1473.View/Download from: Publisher's site
Coral communities were examined from highly turbid near-shore marginal reefs of Abrolhos (Brazil) to test a paradigm previously developed from observations in clear water reefs; specifically, that coral photobiological properties follow a highly conserve
Brading, P, Warner, ME, Davey, P, Smith, DJ, Achterberg, EP & Suggett, DJ 2011, 'Differential effects of ocean acidification on growth and photosynthesis among phylotypes of Symbiodinium (Dinophyceae)', Limnology And Oceanography, vol. 56, no. 3, pp. 927-938.View/Download from: Publisher's site
We investigated the effect of elevated partial pressure of CO2 (pCO(2)) on the photosynthesis and growth of four phylotypes (ITS2 types A1, A13, A2, and B1) from the genus Symbiodinium, a diverse dinoflagellate group that is important, both free-living a
Kerrison, P, Hall-Spencer, JM, Suggett, DJ, Hepburn, LJ & Steinke, M 2011, 'Assessment of pH variability at a coastal CO2 vent for ocean acidification studies', Estuarine Coastal And Shelf Science, vol. 94, no. 2, pp. 129-137.View/Download from: Publisher's site
Marine environments with naturally high CO2 concentrations have become important research sites for studying the impacts of future ocean acidification on biological processes. We conducted high temporal resolution pH and temperature measurements in and around a shallow (2.5-3 m) CO2 vent site off Ischia, Italy in May and June 2008. Loggers were deployed at five stations to monitor water at both the surface and benthos. Our reference station, 500 m from the CO2 vent, had no noticeable vent influence. It had a naturally high and stable benthic pH (mean 8.16, inter-quartile range (IQ): 8.14-8.18) fluctuating with diel periodicity, presumably driven by community photosynthesis and respiration. A principal component analysis (PCA) revealed that the pH of this station was well constrained by meteorological parameters. In contrast, a station positioned within the vent zone, had a low and very variable benthic mean pH of 7.11 (IQ: 6.91-7.62) with large pH fluctuations not well constrained by a PCA. Any stations positioned within 20 m of the main vent zone had lowered pH, but suffered from abnormally large pH fluctuations making them unsuitable representatives to predict future changes to a shallow coastal environment. Between these extremes, we identified a benthic area with a lower pH of 7.84 (IQ: 7.83-7.88) that retained many of the characteristics of the reference station such as a natural diel pH periodicity and low variability. Our results indicate that a range of pH environments maybe commonplace near CO2 vents due to their characteristic acidification of benthic water over a wide area. Such environments could become invaluable natural laboratories for ocean acidification research, closely mimicking future CO2 conditions in a natural setting
Steinke, M, Brading, P, Kerrison, P, Warner, ME & Suggett, DJ 2011, 'Concentrations of dimethylsulfoniopropionate and dimethyl sulfide are strain-specific in symbiotic dinoflagellates (Symbiodinium sp., Dinophyceae)', Journal Of Phycology, vol. 47, no. 4, pp. 775-783.View/Download from: Publisher's site
Dimethyl sulfide (DMS) and dimethylsulfoniopropionate (DMSP) are sulfur compounds that may function as antioxidants in algae. Symbiotic dinoflagellates of the genus Symbiodinium show strain-specific differences in their susceptibility to temperature-induced oxidative stress and have been shown to contain high concentrations of DMSP. We investigated continuous cultures of four strains from distinct phylotypes (A1, A13, A2, and B1) that can be characterized by differential thermal tolerances. We hypothesized that strains with high thermal tolerance have higher concentrations of DMSP and DMS in comparison to strains with low thermal tolerance. DMSP concentrations were strain-specific with highest concentrations occurring in A1 (225 +/- 3.5 mmol . L(-1) cell volume [CV]) and lowest in A2 (158 +/- 3.8 mmol L) 1 CV). Both strains have high thermal tolerance. Strains with low thermal tolerance (A13 and B1) showed DMSP concentrations in between these extremes (194 +/- 19.0 and 160 +/- 6.1 mmol L(-1) CV, respectively). DMS data further confirmed this general pattern with high DMS concentrations in A1 and A13 (4.1 +/- 1.22 and 2.1 +/- 0.37 mmol . L(-1) CV, respectively) and low DMS concentrations in A2 and B1 (0.3 +/- 0.06 and 0.5 +/- 0.22 mmol . L(-1) CV, respectively). Hence, the strain-specific differences in DMSP and DMS concentrations did not match the different abilities of the four phylotypes to withstand thermal stress. Future work should quantify the possible dynamics in DMSP and DMS concentrations during periods of high oxidative stress in Symbiodinium sp. and address the role of these antioxidants in zooxanthellate cnidarians.
Coral bleaching is a major concern to researchers, conservationists and the general public worldwide. To date, much of the high profile attention for bleaching has coincided with major environmental impacts and for many the term coral bleaching is synonymously associated with coral mortality (so-called `lethal bleaching episodes). While this synonymous association has undoubtedly been key in raising public support, it carries unfair representation: nonlethal bleaching is, and always has been, a phenomenon that effectively occurs regularly in nature as corals acclimatize to regular periodic changes in growth environment (days, seasons etc). In addition, corals can exhibit sublethal bleaching during extreme environmental conditions whereby mortality does not occur and corals can potentially subsequently recover once ambient environmental conditions return. Perhaps not surprisingly it is the frequency and extent of these non and sublethal processes that yield key evidence as to how coral species and reef systems will likely withstand environmental and thus climatic change. Observations of non and sublethal bleaching (and subsequent recovery) are arguably not as readily reported as those of lethal bleaching since (1) the convenient tools used to quantify bleaching yield major ambiguity (and hence high potential for misidentification) as to the severity of bleaching; and (2) lethal bleaching events inevitably receive higher profile (media) attention and so are more readily reported. Under-representation of non and sublethal bleaching signs may over-classify the severity of bleaching, under-estimate the potential resilience of reefs against environmental change, and thus ultimately limit (if not depreciate) the validity and effectiveness of reef management policies and practices. While bleaching induced coral mortality must remain our key concern it must be better placed within the context of bleaching signs that do not result in a long-term loss of reefviability.
Exton, DA, Smith, DJ, McGenity, TJ, Steinke, M, Hills, AJ & Suggett, DJ 2010, 'Application of a Fast Isoprene Sensor (FIS) for measuring isoprene production from marine samples', Limnology And Oceanography-methods, vol. 8, pp. 185-195.View/Download from: Publisher's site
Research into isoprene production from marine sources traditionally relies on gas chromatography techniques which are labor intensive, provide a slow sample turnover, and require significant method training. An alternative method is the use of a Fast Iso
Hennige, SJ, Smith, DJ, Walsh, S, McGinley, MP, Warner, ME & Suggett, DJ 2010, 'Acclimation and adaptation of scleractinian coral communities along environmental gradients within an Indonesian reef system', Journal Of Experimental Marine Biology And Ecology, vol. 391, no. 1-2, pp. 143-152.View/Download from: Publisher's site
In 2007 and 2008, multiple sites were identified in the Wakatobi Marine National Park, South East Sulawesi, Indonesia, which each represented a point along a gradient of light quality, temperature and turbidity. This gradient included `optimal, intermediate and marginal sites, where conditions were close to the survival threshold limit for corals. Coral communities changed across this gradient from diverse, mixed growth form assemblages to specialised, massive growth form dominated communities. The massive coral Goniastrea aspera was the only species identified at the most marginal and optimal sites. Branching species Acropora formosa and Porites cylindrica were only identified at optimal sites. The in hospite Symbiodinium community also changed across the environmental gradient from members of the Symbiodinium clade C on optimal reefs (in branching and massive species) to clade D on marginal reefs (in massive species). Substantial variability in respiration and photosynthesis was observed in massive coral species under different environmental conditions, which suggests that all corals cannot be considered equal across environments. Studying present-day marginal environments is crucial to further understanding of future reef bio-diversity, functioning and accretion, and from work presented here, it is likely that as future climate change extends marginal reef range, branching coral diversity may decrease relative to massive, more resilient corals.
Ragni, M, Airs, R, Hennige, SJ, Suggett, DJ, Warner, ME & Geider, RJ 2010, 'PSII photoinhibition and photorepair in Symbiodinium (Pyrrhophyta) differs between thermally tolerant and sensitive phylotypes', Marine Ecology Progress Series, vol. 406, pp. 57-70.View/Download from: Publisher's site
Cnidarians containing symbiotic microalgae often inhabit highly variable light environments where successful growth requires that, during transient (potentially stressful) periods of high light (HL), the microalgal cells invest energy in photoprotection to minimise photodamage, or allow for photodamage to occur and invest in photorepair; however, the relative contribution of photoprotection and photorepair remains uncharacterised. Here we determined the light dependence of Photosystem II (PSII) photoinhibition and photorepair in 2 phylotypes of Symbiodinium displaying different susceptibilities to thermal stress. Upon exposure to photon flux densities (PFDs) >500 µmol photons m2 s1 the thermally `sensitive Strain A1.1 displayed higher net photoinhibition, measured as a decrease in maximum PSII efficiency (Fv/Fm), than the thermally `tolerant Strain A1. In contrast, gross photoinhibition, assessed as the decline of Fv/Fm in the presence of an inhibitor of D1 protein synthesis, was similar in the 2 strains. Therefore, photorepair was considered to be the key mechanism minimising net photoinhibition in Strain A1. Consistent with this conclusion, the 2 strains displayed similar capacities for other mechanisms of avoiding photodamage, specifically, photochemical (qP) and non-photochemical (NPQ) excitation energy quenching. Measurements on Strain A1 grown under 2 PFDs (100 and 650 µmol photons m2 s1) revealed that photoacclimation to HL involved the upregulation of qP, which minimised gross photoinhibition by maintaining PSII in a more oxidised state. We conclude that both interspecific (e.g. phylotype diversity) and intraspecific (e.g. photoacclimation state) factors affect the susceptibility of Symbiodinium to light stress.
Acuna Alvarez, L, Exton, D, Timmis, K, Suggett, DJ & Mcgenity, T 2009, 'Characterization Of Marine Isoprene-degrading Communities', Environmental Microbiology, vol. 11, no. 12, pp. 3280-3291.View/Download from: Publisher's site
P>Isoprene is a volatile and climate-altering hydrocarbon with an atmospheric concentration similar to that of methane. It is well established that marine algae produce isoprene; however, until now there was no specific information about marine isoprene sinks. Here we demonstrate isoprene consumption in samples from temperate and tropical marine and coastal environments, and furthermore show that the most rapid degradation of isoprene coincides with the highest rates of isoprene production in estuarine sediments. Isoprene-degrading enrichment cultures, analysed by denaturing gradient gel electrophoresis and 454 pyrosequencing of the 16S rRNA gene and by culturing, were generally dominated by Actinobacteria, but included other groups such as Alphaproteobacteria and Bacteroidetes, previously not known to degrade isoprene. In contrast to specialist methane-oxidizing bacteria, cultivated isoprene degraders were nutritionally versatile, and nearly all of them were able to use n-alkanes as a source of carbon and energy. We therefore tested and showed that the ubiquitous marine hydrocarbon-degrader, Alcanivorax borkumensis, could also degrade isoprene. A mixture of the isolates consumed isoprene emitted from algal cultures, confirming that isoprene can be metabolized at low, environmentally relevant concentrations, and suggesting that, in the absence of spilled petroleum hydrocarbons, algal production of isoprene could maintain viable populations of hydrocarbon-degrading microbes. This discovery of a missing marine sink for isoprene is the first step in obtaining more robust predictions of its flux, and suggests that algal-derived isoprene provides an additional source of carbon for diverse microbes in the oceans.
Bar-zeev, E, Berman-frank, I, Stambler, N, Vazquez-dominguez, E, Zohary, T, Capuzzo, E, Meeder, E, Suggett, DJ, Iluz, D, Dishon, G & Berman, T 2009, 'Transparent exopolymer particles (TEP) link phytoplankton and bacterial production in the Gulf of Aqaba', Aquatic Microbial Ecology, vol. 56, no. 2-3, pp. 217-225.View/Download from: Publisher's site
Variations in transparent exopolymer particles (TEP), bacterial biomass production (BP) and primary productivity (PP) were followed over 52 h at a deep water station in the Gulf of Aqaba (Eilat, Israel) during the spring, in April 2008. About 20 h after the start of the study, there was a short (~15 h) but intense storm event that probably caused a nutrient pulse and, subsequently, a brief outgrowth of diatoms in the euphotic layer. Concentrations of TEP and BP ranged from 23 to 228 µg gum xanthan equivalents l1 and from 0.2 to 0.6 µg C l1 h1, respectively. Concentrations of TEP and BP were measured in unfiltered and in GF/C (1.2 µm)-prefiltered samples. Most of the TEP (59 ± 21% of total TEP, mean ± SD) were in the smaller (GF/C-filtered) size fraction (0.41.2 µm); however, after the crash of the diatom bloom, the majority of TEP were in the >1.2 µm size fraction. In the GF/C-filtered fraction, BP averaged 59 ± 12% and 93 ± 5% of total BP in the upper water column and from 300 m, respectively. Significant correlations were observed between TEP and BP, suggesting that active heterotrophic bacteria may have been associated with these particles. During the 3 d of our study, PP and BP in the euphotic zone averaged 480 and 225 mg C m2 d1, respectively, suggesting that about half or more of the primary produced carbon was metabolized by heterotrophic bacteria in the upper water column. Coincident with strong mixing caused by the storm, TEP concentrations decreased in the surface water and increased at depth. We suggest that TEP acted to link carbon flux between the primary producers and heterotrophic bacteria, and that the downward movement of TEP from the upper water layers may be an important process in transferring organic carbon to deeper waters of the Gulf of Aquaba. Sinking TEP could provide not only organic carbon substrates for associated bacteria but also form `hot spots of elevated microbial metabolism and nutrient cycling throughout the water column.
Haapkyla, J, Seymour, AS, Barneah, O, Brickner, I, Hennige, S, Suggett, DJ & Smith, D 2009, 'Association of Waminoa sp. (Acoela) with corals in the Wakatobi Marine Park, South-East Sulawesi, Indonesia', Marine Biology, vol. 156, no. 5, pp. 1021-1027.View/Download from: Publisher's site
This is the first quantitative study on the prevalence of epizoic Waminoa sp. acoel worms and their association with corals in the Wakatobi Marine National Park (WMNP), South-East Sulawesi, Indonesia. Three replicate transects were laid on the reef crest
Hennige, SJ, Suggett, DJ, Warner, ME, McDougall, KE & Smith, DJ 2009, 'Photobiology of Symbiodinium revisited: bio-physical and bio-optical signatures', Coral Reefs, vol. 28, no. 1, pp. 179-195.View/Download from: Publisher's site
Light is often the most abundant resource within the nutrient-poor waters surrounding coral reefs. Consequently, zooxanthellae (Symbiodinium spp.) must continually photoacclimate to optimise productivity and ensure coral success. In situ coral photobiolo
Moore, C, Mills, M, Achterberg, E, Geider, R, Laroche, J, Lucas, M, Mcdonagh, E, Pan, X, Poulton, A, Rijkenberg, M, Suggett, DJ, Ussher, S & Woodward, E 2009, 'Large-scale Distribution Of Atlantic Nitrogen Fixation Controlled By Iron Availability', Nature Geoscience, vol. 2, no. 12, pp. 867-871.View/Download from: Publisher's site
Oceanic fixed-nitrogen concentrations are controlled by the balance between nitrogen fixation and denitrification(1-4). A number of factors, including iron limitation(5-7), can restrict nitrogen fixation, introducing the potential for decoupling of nitrogen inputs and losses(2,5,8). Such decoupling could significantly affect the oceanic fixed-nitrogen inventory and consequently the biological component of ocean carbon storage and hence air-sea partitioning of carbon dioxide(2,5,8,9). However, the extent to which nutrients limit nitrogen fixation in the global ocean is uncertain. Here, we examined rates of nitrogen fixation and nutrient concentrations in the surfacewaters of the Atlantic Ocean along a north-south 10,000 km transect during October and November 2005. We show that rates of nitrogen fixation were markedly higher in the North Atlantic compared with the South Atlantic Ocean. Across the two basins, nitrogen fixation was positively correlated with dissolved iron and negatively correlated with dissolved phosphorus concentrations. We conclude that inter-basin differences in nitrogen fixation are controlled by iron supply rather than phosphorus availability. Analysis of the nutrient content of deep waters suggests that the fixed nitrogen enters North Atlantic Deep Water. Our study thus supports the suggestion that iron significantly influences nitrogen fixation(5), and that subsequent interactions with ocean circulation patterns contribute to the decoupling of nitrogen fixation and loss(2,4,8).
Suggett, DJ, MacIntyre, HL, Kana, TM & Geider, RJ 2009, 'Comparing electron transport with gas exchange:parameterising exchange rates between alternative photosynthetic currencies for eukaryotic phytoplankton', Aquatic Microbial Ecology, vol. 56, no. 2-3, pp. 147-162.View/Download from: Publisher's site
Estimates of aquatic primary productivity derived from in situ active chl a fluorescence have rapidly gained popularity over the past 2 decades. This trend has been driven primarily by the need to improve upon `conventional carbon (C) uptake- or oxygen (O2) evolution-based productivity estimates that require water samples to be incubated ex situ. Unlike the conventional approaches to measuring productivity, chlorophyll fluorescence measurements inherently describe only the activity of photosystem II (PSII) in the light reactions; thus, the photosynthetic `currency of the fluorescence based approach is an electron turnover rate for PSII (ETRPSII). A photosynthetic currency of electrons has limited ecological relevance but can be converted to a currency of carbon if an `exchange rate, i.e. a value or factor of equivalence for any single time point, is applied. We used fast repetition rate fluorometry (FRRf), mass inlet membrane spectrometry (MIMS) and 14C uptake to determine ETRPSII, gross and net O2 evolution and C fixation measured simultaneously for 6 microalgal species and for different steady-state growth conditions. Quantifying the PSII reaction centre (RCII) concentration and the spectral dependency of the effective absorption cross section yielded an FRRf approach that provided a robust estimate of the ETRPSII and gross O2 evolution for all species and conditions tested; however, the ETRPSII exceeded carbon dioxide (CO2) uptake by a factor of ~5.4 to 11.6. At least 3 species exhibited substantial light-dependent O2 cycling to account for ~40 to 60% of the difference between the ETRPSII and CO2 uptake. The highly variable nature of the ETRPSII:CO2 uptake `exchange rate observed here highlights the need for future studies that rely on active fluorescence to examine aquatic productivity to focus towards a systematic description of how electrons are coupled to C fixation in nature
Suggett, DJ, Moore, CM, Hickman, AE & Geider, RJ 2009, 'Interpretation of fast repetition rate (FRR) fluorescence: signatures of phytoplankton community structure versus physiological state', Marine Ecology Progress Series, vol. 376, pp. 1-19.View/Download from: Publisher's site
Introduction of active chlorophyll a fluorescence protocols, in particular fast repetition rate (FRR) fluorometry, to oceanography and limnology 15 yr ago has enabled rapid assessment of photosynthetic physiology in situ. The FRR protocol generates simultaneous measurements of Photosystem II (PSII) effective absorption cross sections (termed sPSII) and photochemical efficiency (termed Fv/Fm). Both Fv/Fm and sPSII measurements have been utilised to examine the effects of physiological stress on the photosynthetic apparatus of phytoplankton in an ever growing number of fluorescence-based studies. However, it is now becoming clearer that in situ values of Fv/Fm and sPSII also contain taxonomic information. Here, we present a synthesis of previously unpublished and published data, which show that Fv/Fm and sPSII vary principally with broad-scale changes in community structure. These patterns observed in situ conform to trends observed in laboratory-grown cultures of a range of phytoplankton taxa. The magnitudes of variability in Fv/Fm and sPSII driven by changes in phytoplankton community structure often exceed that induced by nutrient limitation (as determined from controlled nutrient addition experiments). An exception to this general trend occurs in high-nutrient, low-chlorophyll a (HNLC) regions, where strong phenotypic changes in Fv/Fm and sPSII have been repeatedly demonstrated on relief of iron limitation. Overall, FRR fluorescence measurements of both Fv/Fm and sPSII in natural populations represent a combination of the taxonomic `signature (values of Fv/Fm and sPSII determined by the taxa present) within the phytoplankton community that is further modified according to the (photo-) physiological status. As such, fluorescence-based investigations of mixed populations must account for potential variations in phytoplankton community structure before interpretations of physiological status are made.
Hennige, SJ, Smith, DJ, Perkins, R, Consalvey, M, Paterson, DM & Suggett, DJ 2008, 'Photoacclimation, growth and distribution of massive coral species in clear and turbid waters', Marine Ecology Progress Series, vol. 369, pp. 77-88.View/Download from: Publisher's site
Massive coral species play a key role in coral reef ecosystems, adding significantly to physical integrity, long term stability and reef biodiversity. This study coupled the assessment of the distribution and abundance of 4 dominant massive coral species, Diploastrea heliopora, Favia speciosa, F. matthaii and Porites lutea, with investigations into species-specific photoacclimatory responses within the Wakatobi Marine National Park of southeast Sulawesi, Indonesia, to determine the potential of photoacclimation to be a driver of biological success. For this, rapid light curves using pulse amplitude modulated (PAM) chlorophyll a fluorescence techniques were employed with additional manipulations to circumvent differences of light quality and absorption between species and across environmental gradients. P. lutea was examined over a range of depths and sites to determine patterns of photoacclimation, and all 4 species were assessed at a single depth between sites for which long-term data for coral community structure and growth existed. Light availability was more highly constrained with depth than between sites; consequently, photoacclimation patterns for P. lutea appeared greater with depth than across environmental gradients. All 4 species were found to differentially modify the extent of non-photochemical quenching to maintain a constant photochemical operating efficiency (qP). Therefore, our results suggest that these massive corals photoacclimate to ensure a constant light-dependent rate of reduction of the plastoquinone pool across growth environments.
Prasil, O, Suggett, DJ, Cullen, JJ, Babin, M & Govindjee, D 2008, 'Aquafluo 2007: chlorophyll fluorescence in aquatic sciences, an international conference held in Nové Hrady', Photosynthesis Research, vol. 95, no. 1, pp. 111-115.View/Download from: Publisher's site
Ross, ON, Moore, CM, Suggett, DJ, MacIntyre, HL & Geider, RJ 2008, 'A model of photosynthesis and photo-protection based on reaction center damage and repair', Limnology and Oceanography, vol. 53, no. 5, pp. 1835-1852.View/Download from: Publisher's site
Phytoplankton photosynthesis under the rapidly fluctuating irradiance which results from turbulent mixing through the vertical light gradient is poorly understood. Ship-based measurements often apply the fast repetition rate fluorescence (FRRF) technique in situ or in vivo to gauge the physiological state of the phytoplankton community and infer some of the physical properties of the water column (such as mixing time scales). We describe the development and validation of a model of photosynthetic electron turnover at photosystemII with consideration of downstream limitation, based on the redox state of photosystem II. We also include empirical formulations for slower processes such as photo-protection (from nonphotochemical quenching) and photo-inhibition. By confronting the simple model with laboratory data for Dunaliella tertiolecta, we were able to refine the model so that it faithfully produced rates of photosynthetic electron transfer determined by FRR fluorescence. Further, we were able to validate the model estimates of linear photosynthetic electron transfer rates against completely independent measurements obtained using 14C-bicarbonate assimilation in photosynthesis-light curves.
Suggett, DJ, Warner, ME, Smith, DJ, Davey, P, Hennige, S & Baker, NR 2008, 'Photosynthesis and production of hydrogen peroxide by Symbiodinium (Pyrrhophyta) phylotypes with different thermal tolerances', Journal Of Phycology, vol. 44, no. 4, pp. 948-956.View/Download from: Publisher's site
Occurrences whereby cnidaria lose their symbiotic dinoflagellate microalgae (Symbiodinium spp.) are increasing in frequency and intensity. These socalled bleaching events are most often related to an increase in water temperature, which is thought to limit certain Symbiodinium phylotypes from effectively dissipating absorbed excitation energy that is otherwise used for photochemistry. Here, we examined photosynthetic characteristics and hydrogen peroxide (H2O2) production, a possible signal involved in bleaching, from two Symbiodinium types (a thermally ``tolerant A1 and ``sensitive B1) representative of cnidariaSymbiodinium symbioses of reef-building Caribbean corals. Under steady-state growth at 26C, a higher efficiency of PSII photochemistry, rate of electron turnover, and rate of O2 production were observed for type A1 than for B1. The two types responded very differently to a period of elevated temperature (32C): type A1 increased light-driven O2 consumption but not the amount of H2O2 produced; in contrast, type B1 increased the amount of H2O2 produced without an increase in light-driven O2 consumption. Therefore, our results are consistent with previous suggestions that the thermal tolerance of Symbiodinium is related to adaptive constraints associated with photosynthesis and that sensitive phylotypes are more prone to H2O2 production. Understanding these adaptive differences in the genus Symbiodinium will be crucial if we are to interpret the response of symbiotic associations, including reef-building corals, to environmental change.
Suggett, DJ, Le Floc'h, E, Harris, GN, Leonardos, N & Geider, RJ 2007, 'Different strategies of photoacclimation by two strains of Emiliania huxleyi (Haptophyta)', Journal Of Phycology, vol. 43, no. 6, pp. 1209-1222.View/Download from: Publisher's site
Photoacclimation involves the modification of components of the light and dark reactions to optimize photosynthesis following changes in available light. All of the energy required for photosynthesis comes from linear electron transport through PSII and
Moore, CM, Suggett, DJ, Hickman, AE, Kim, Y, Tweddle, JF, Sharples, J, Geider, RJ & Holligan, PM 2006, 'Phytoplankton photoacclimation and photoadaptation in response to environmental gradients in a shelf sea', Limnology And Oceanography, vol. 51, no. 2, pp. 936-949.View/Download from: Publisher's site
Variability in the photosynthetic performance of natural phytoplankton communities, due to both taxonomic composition and the physiological acclimation of these taxa to environmental conditions, was assessed at contrasting sites within a temperate shelf
Suggett, DJ, Maberly, SC & Geider, RJ 2006, 'Gross photosynthesis and lake community metabolism during the spring phytoplankton bloom', Limnology And Oceanography, vol. 51, no. 5, pp. 2064-2076.View/Download from: Publisher's site
Daily productivity determinations of linear photosynthetic electron transfer and of net and gross inorganic CO2 uptake were determined in situ throughout a 6-week sampling period of the spring phytoplankton bloom in Esthwaite Water in the English Lake Di
Suggett, DJ, Moore, CM, Maranon, E, Omachi, C, Varela, RA, Aiken, J & Holligan, PM 2006, 'Photosynthetic electron turnover in the tropical and subtropical Atlantic Ocean', Deep Sea Research Part II: Topical Studies in Oceanography, vol. 53, no. 14-16, pp. 1573-1592.View/Download from: Publisher's site
Photosynthetic electron transport directly generates the energy required for carbon fixation and thus underlies the aerobic metabolism of aquatic systems. We determined photosynthetic electron turnover rates, ETRs, from ca. 100 FRR fluorescence water-column profiles throughout the subtropical and tropical Atlantic during six Atlantic Meridional Transect cruises (AMT 6, MayJune 1998, to AMT 11, SeptemberOctober 2000). Each FRR fluorescence profile yielded a water-column ETR-light response from which the maximum electron turnover rate (ETRRCIImax), effective absorption (s PSII) and light saturation parameter (E k) specific to the concentration of photosystem II reaction centres (RCIIs) were calculated. ETRRCIImax and E k increased whilst s PSII decreased with mixed-layer depth and the daily integrated photosynthetically active photon flux when all provinces were considered together. These trends suggested that variability in maximum ETR can be partly attributed to changes in effective absorption. Independent bio-optical measurements taken during AMT 11 demonstrated that s PSII variability reflects taxonomic and physiological differences in the phytoplankton communities. ETRRCIImax and Ek, but not sPSII, remained correlated with mixed-layer depth and daily integrated photosynthetically active photon flux when data from each oceanic province were considered separately, indicating a decoupling of electron turnover and carbon fixation rates within each province. Comparison of maximum ETRs with 14C-based measurements of Pmax further suggests that light absorption and C fixation are coupled to differing extents for the various oligotrophic Atlantic provinces. We explore the importance of quantifying RCII concentration for determination of ETRs and interpretation of ETR-C fixation coupling.
Smith, DJ, Suggett, DJ & Baker, NR 2005, 'Is photoinhibition of zooxanthellae photosynthesis the primary cause of thermal bleaching in corals?', Global Change Biology, vol. 11, no. 1, pp. 1-11.View/Download from: Publisher's site
The bleaching of corals in response to increases in temperature has resulted in significant coral reef degradation in many tropical marine ecosystems. This bleaching has frequently been attributed to photoinhibition of photosynthetic electron transport a
Macintyre, H, Lomas, M, Cornwell, J, Suggett, DJ, Gobler, C, Koch, E & Kana, T 2004, 'Mediation Of Benthic-pelagic Coupling By Microphytobenthos: An Energy- And Material-based Model For Initiation Of Blooms Of Aureococcus Anophagefferens', Harmful Algae, vol. 3, no. 4, pp. 403-437.View/Download from: Publisher's site
We present a conceptual model for initiation of blooms of the estuarine brown-tide pelagophyte Aureococcus anophagefferens. The model is based on the observation that in addition to its well-documented stimulation by organic nutrients, Aureococcus is pie
Suggett, DJ, MacIntyre, HL & Geider, RJ 2004, 'Evaluation of biophysical and optical determinations of light absorption by photosystem II in phytoplankton', Limnology And Oceanography-methods, vol. 2, pp. 316-332.View/Download from: Publisher's site
Fast repetition rate (FRR) fluorescence can provide highly resolved estimates of light absorption by photosystem II ( PSII), a variable that is critical to bio-optical determinations of phytoplankton productivity. We compared estimates of chlorophyll a -
Davey, MS, Suggett, DJ, Geider, RJ & Taylor, AR 2003, 'Phytoplankton plasma membrane redox activity: Effect of iron limitation and interaction with photosynthesis', Journal Of Phycology, vol. 39, no. 6, pp. 1132-1144.View/Download from: Publisher's site
Phytoplankton plasma membrane electron transport activity was determined by monitoring the reduction of the impermeant artificial electron acceptor ferricyanide in a range of diatoms. The results revealed that constitutive plasma membrane electron transp
Moore, CM, Suggett, DJ, Holligan, PM, Sharples, J, Abraham, ER, Lucas, MI, Rippeth, T, Fisher, NR, Simpson, JH & Hydes, D 2003, 'Physical controls on phytoplankton physiology and production at a shelf sea front: a fast repetition-rate fluorometer based field study', Marine Ecology Progress Series, vol. 259, pp. 29-45.View/Download from: Publisher's site
Observations of phytoplankton physiology collected using a fast repetition-rate fluorometer (FRRF) in the vicinity of a shelf-sea tidal-mixing front are presented. These data are combined with more traditional 14C-based measurements and observations of environmental parameters, including estimates of turbulent dissipation rates, in order to investigate the influence of physical forcing on the productivity of the system. Low nutrient concentrations on the stratified side of the front result in a reduction of photosynthetic efficiency. Conversely, the high degree of vertical mixing on the mixed side of the front constrains the ability of phytoplankton to adjust their photosynthetic apparatus to the ambient irradiance field. Redistribution of phytoplankton biomass and variations in physiological parameters also result from the spring-neap tidal cycle. FRRF- and 14C-derived physiological measurements are compared in the context of environmental gradients in the region. A strong correlation was found between independently measured functional absorption cross-sections (sPSII) and maximal photosynthetic rates (P*max). Such a relationship was unlikely to have been causative and may have resulted from shifts in the balance between light-harvesting and carbon fixation across the front. The association of changes in P*max with variations in sPSII provided the basis for the development of an empirical model, specific to the system and time of study, which utilised FRRF data to extrapolate between primary productivity rates measured at fixed sites. When applied to high resolution cross-frontal data, the model suggested small-scale variations in productivity related to both spatial and temporal physical forcing including the spring-neap cycle.
Suggett, DJ, Oxborough, K, Baker, N, Macintyre, H, Kana, T & Geider, R 2003, 'Fast Repetition Rate And Pulse Amplitude Modulation Chlorophyll A Fluorescence Measurements For Assessment Of Photosynthetic Electron Transport In Marine Phytoplankton', European Journal Of Phycology, vol. 38, no. 4, pp. 371-384.View/Download from: Publisher's site
Pulse amplitude modulation (PAM) and fast repetition rate (FRR) fluorescence are currently used to estimate photosynthetic quantum yields and photosynthetic rates in aquatic systems. Here we compare simultaneous measurements of the photochemical efficien
Suggett, DJ, Kraay, G, Holligan, P, Davey, M, Aiken, J & Geider, R 2001, 'Assessment of photosynthesis in a spring cyanobacterial bloom by use of a fast repetition rate fluorometer', Limnology And Oceanography, vol. 46, no. 4, pp. 802-810.View/Download from: Publisher's site
Estimates of gross primary production (GPP) based on fast repetition rate fluorometer (FRRF) measurements were compared with independent C-14 and O-2 at three stations during a spring bloom in the North Atlantic. A photosynthesis versus irradiance (P-E)
Gibb, SW, Barlow, RG, Cummings, DG, Rees, NW, Trees, CC, Holligan, P & Suggett, D 2000, 'Surface phytoplankton pigment distributions in the Atlantic Ocean: An assessment of basin scale variability between 50°N and 50°S', Progress in Oceanography, vol. 45, no. 3-4, pp. 339-368.View/Download from: Publisher's site
We present an overview of the spatial distributions of phytoplankton pigments along transects between the UK and the Falkland Islands. These studies, undertaken as a component of the UK Atlantic Meridional Transect (AMT) programme, provided the first post-launch validation data for the NASA SeaWiFS satellite. Pigment data are used to characterise basin-scale variations in phytoplankton biomass and community composition over 100°of latitude, and to compliment the definition of hydrographic oceanic provinces. A summary of the key pigment characteristics of each province is presented. Concentrations of total chlorophyll a (totCHLa = chlorophyll a, CHLa + divinyl CHLa, dvCHLa) were greatest in high latitude temperate waters (>37°N and >35°S), and in the Canary Current Upwelling system. In these regions, the total carotenoid (totCAR) budget was dominated by photosynthetic carotenoids (PSCs). High accessory pigment diversity was observed of which fucoxanthin (FUC), 19'-hexanoyloxyfucoxanthin (HEX), and diadinoxanthin (DIAD) were most abundant, indicating proliferation of large eukaryotes and nanoflagellates. In contrast, tropical and sub-tropical waters exhibited concentrations of totCHLa below 500 ng l-1, with the North Atlantic Sub-tropical East gyre (NASE, 26.7-35°N), South Equatorial Current (SeqC, 7-14.6°S) and South Atlantic tropical Gyre (SATG, 14.6-26°S) characterised by totCHLa of <100 ng-1. These waters exhibited relatively limited pigment diversity, and the totCAR budget was dominated by photoprotecting pigments (PPCs) of which zeaxanthin (ZEA), a marker of prokaryotes (cyanobacteria and prochlorophytes), was most abundant. DvCHLa, a marker of prochlorophytes was detected in waters at temperatures >15°C, and between the extremes of 48°N and 42°S. DvCHLa accounted for up to two-thirds of totCHLa in oligotrophic provinces demonstrating the importance of prochlorophytes to oceanic biomass. Overall, HEX was the dominant PSC, contributing up to 75% of totCAR. HEX...
Poulton, AJ, Stinchcombe, MC, Achterberg, EP, Bakker, DCE, Dumousseaud, C, Lawson, HE, Lee, GA, Richier, S, Suggett, DJ & Young, JR, 'Coccolithophores on the north-west European shelf: calcification rates and environmental controls', Biogeosciences Discussions, vol. 11, no. 2, pp. 2685-2733.View/Download from: Publisher's site
Abstract. Coccolithophores are a key functional group in terms of the pelagic production of calcium carbonate (calcite), although their contribution to shelf-sea biogeochemistry, and how this relates to environmental conditions, is poorly constrained. Measurements of calcite production (CP) and coccolithophore abundance were made on the north-west European shelf to examine trends in coccolithophore calcification along natural gradients of carbonate chemistry, macronutrient availability and plankton composition. Similar measurements were also made in three bioassay experiments where nutrient (nitrate, phosphate) and pCO2 levels were manipulated. Nanoflagellates (< 10 μm) dominated chlorophyll biomass and primary production (PP) at all but one sampling site, with CP ranging from 0.6–9.6 mmol C m−2d−1. Highest CP and coccolithophore cell abundance occurred in a diatom bloom in fully mixed waters off Helgoland, rather than in two distinct coccolithophore blooms in the central North Sea and Western English Channel. Estimates of coccolithophore contributions to total PP and nanoplankton PP were generally < 5%, apart from in a coccolithophore bloom at the Western English Channel Observatory (E1) where coccolithophores contributed up to 11% and at Helgoland where they contributed ~23% to nanoplankton PP. Variability in CP was influenced by cell numbers, species composition and cell-normalised calcification rates under both in situ conditions and in the experimental bioassays. Water column structure and light availability had a strong influence on cellular calcification, whereas nitrate (N) to phosphate (P) ratios influenced bulk CP. Coccolithophore communities in the northern North Sea and over the Norwegian Trench showed responses to N and P addition whereas oceanic communities in the Bay of Biscay showed no response. Sharp decreases in pH and a rough halving of calcite saturation states in the bioassay experiments led to decreased CP in the Bay of Biscay ...
Richier, S, Achterberg, EP, Dumousseaud, C, Poulton, AJ, Suggett, DJ, Tyrrell, T, Zubkov, MV & Moore, CM, 'Carbon cycling and phytoplankton responses within highly-replicated shipboard carbonate chemistry manipulation experiments conducted around Northwest European Shelf Seas', Biogeosciences Discussions, vol. 11, no. 3, pp. 3489-3534.View/Download from: Publisher's site
Abstract. The ongoing oceanic uptake of anthropogenic carbon dioxide (CO2) is significantly altering the carbonate chemistry of seawater, a phenomenon referred to as ocean acidification. Experimental manipulations have been increasingly used to gauge how continued ocean acidification will potentially impact marine ecosystems and their associated biogeochemical cycles in the future; however, results amongst studies, particularly when performed on natural communities, are highly variable, which in part likely reflects inconsistencies in experimental approach. To investigate the potential for identification of more generic responses and greater experimentally reproducibility, we devised and implemented a series of highly replicated (n = 8), short term (2–4 days) multi-level (&geq; 4 conditions) carbonate chemistry/nutrient manipulation experiments on a range of natural microbial communities sampled in Northwest European shelf seas. Carbonate chemistry manipulations and resulting biological responses were found to be highly reproducible within individual experiments and to a lesser extent between geographically different experiments. Statistically robust reproducible physiological responses of phytoplankton to increasing pCO2, characterized by a suppression of net growth for small sized cells (< 10 μm), were observed in the majority of the experiments, irrespective of nutrient status. Remaining between-experiment variability was potentially linked to initial community structure and/or other site-specific environmental factors. Analysis of carbon cycling within the experiments revealed the expected increased sensitivity of carbonate chemistry to biological processes at higher pCO2 and hence lower buffer capacity. The results thus emphasize how biological-chemical feedbacks may be altered in the future ocean.
Warner, ME & Suggett, DJ 2016, 'The photobiology of symbiodinium spp.: Linking physiological diversity to the implications of stress and resilience' in The Cnidaria, past, present and Future: The World of Medusa and her Sisters, pp. 489-509.View/Download from: Publisher's site
© Springer International Publishing Switzerland 2016. Over the past two decades, our knowledge of Symbiodinium genetic diversity and ecological distribution has grown at an incredible pace, while the physiological diversity and how it may be compared to the phylogenetic and evolutionary constraints of these dinoflagellates is still catching up. Our knowledge of the photobiology of Symbiodinium has been driven largely by the desire to explain cellular mechanisms of stress and reaction to global events driven by climate change. We tend to focus more on the former in this chapter since this is where the majority of work has been performed to date. However, it is imperative that we return to the first principles of phytoplankton physiological ecology to form a more complete understanding of the photobiology of these algae and the physiological constraints expected in an algal cell capable of living benthic, pelagic and symbiotic life styles. In order to understand not only the pathways of cellular dysfunction, but also resilience, we address specific patterns of light harvesting balance, reaction centre turnover, inorganic carbon acquisition and utilisation, and alternative electron transport across this genus. Symbiodinium - focused research, and physiology in particular, poses several challenges that demand a broad perspective gleaned from working with these algae in culture as well as in hospite in controlled 'model' and natural cnidarian hosts across many different environments. We outline several key processes related to photosynthesis as well as new directions integrating many of these processes that are required to more fully understand how environmental change shapes the role of photobiology in governing the ecological success of these important algae.
© Springer Science+Business Media New York 2014. Marine phytoplankton account for about 45 % of global net primary production (NPP). In addition, they perform other important biogeochemical functions including nitrogen fixation, calcium carbonate precipitation, and the production of climatically active gases such as dimethyl sulfide. Oceanographers employ a wide variety of platforms for studying marine phytoplankton ecology, including sampling from ships, sampling from autonomous remotely operated vehicles, and collecting observations from Earthorbiting satellites. Marine phytoplankton range in size from < 1μm in diameter to about 1 mm in length and include representatives from at least five eukaryotic phyla together with the cyanobacteria. This wide size range and phylogenetic diversity presents challenges for quantifying and characterizing phytoplankton communities. Functional traits that quantify responses of growth rate, photosynthesis and nutrient uptake to temperature, irradiance, and nutrient availability provide a useful basis for understanding phytoplankton ecology. A variety of complementary approaches are used to measure gross and net primary production. These include measuring production of O 2 and organic matter in bottle experiments and measuring diel and seasonal changes of O 2 in open waters. Information obtained from satellite remote sensing of ocean color is used to calculate NPP on regional and global scales. The physical and chemical variables that drive NPP include temperature, nutrient availability, and solar radiation. These vary in time and space, and our understanding of this variability is largely encapsulated in the concepts of the seasonal production cycle and marine biogeochemical provinces. Nutrient limitation sets an upper limit to NPP over most of the ocean surface, with either inorganic iron or nitrogen being the proximate limiting element in different regions. The upper water column is stably stratified over much of the ocean,...
Hennige, SJ, Suggett, DJ, Hepburn, LJ, Pugsley, A, Crabbe, J & Smith, DJ 2010, 'Coral reefs of the Wakatobi: Processes of reef growth and loss' in Marine Research and Conservation in the Coral Triangle, Nova, USA, pp. 27-44.
Coral reef accretion (net reef growth) is the result of complex and dynamic interactions between reef building organisms, bioeroders and the environment. Coral calcification often forms the 'measureable' portion of net reef growth, and is dependent upon the successful symbiotic relationship between cnidaria and photosynthetic dinoflagellate microalgae (Symbiodinium spp.). Light is thus a crucial resource for coral growth. Processes of reef loss include abiotic (storms, disturbance events and climate change) and biotic (bioeroders and corralivores) factors. To assess reef accretion, an understanding of the relationship between coral growth and loss, environmental variables and biotic eroders is vital. Reef systems that have both high biodiversity and varied environmental conditions are prime 'study grounds' to assess these complex interactions. An important example is the Wakatobi National Park (WNP), which is a centre for biodiversity but also has active long-term projects across a range of environments. This chapter examines the underlying physiology of the coral symbiotic relationship, and how this relates to reef growth by using studies from both the WNP and other systems. Understanding these processes remains a critical first step in any reef management strategy plan, but current methods by which reef growth can be assessed often lack the resolution and accuracy needed to relate to changes of environment. Consequently, this chapter discusses benefits and limitations of certain techniques used to assess reef growth. Ultimately, accurate coral growth measurements will improve our ability to model future ecosystem change, and should therefore be a priority research area to support future reef management and conservation strategies. Processes of reef loss are then discussed with regard to the impact of bioeroders, environmental perturbations and anthropogenic stressors upon primary and secondary reef framework. © 2010 by Nova Science Publishers, Inc. All rights r...
Suggett, DJ, Moore, CM & Geider, RJ 2010, 'Estimating Aquatic Productivity from Active Fluorescence Measurements' in Suggett, DJ, Prasil, O & Borowitzka, M (eds), Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications, Springer Science & Business Media, 15, pp. 103-127.
This book follows on from the first international conference on "chlorophyll fluorescence in the aquatic sciences" (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through ...
Light-absorbing pigments cause many marine organisms to naturally fluoresce, a phenomenon whereby light is emitted at a longer wavelength following light absorption at a shorter wavelength. Fluorescence provides a relatively unobtrusive signature of organism abundance and physiology; consequently, a wide variety of fluorescence techniques are now routinely employed in marine biological investigations. Important technological advances throughout the last 20 years have enabled exploration of the taxonomy and physiology of marine organisms with increasing complexity. In particular, the intracellular use of fluorescent tags, molecules, and dyes that covalently bind to sensing biomolecules. However, many such techniques require samples to be removed from nature. Most widespread for assaying biology in situ exploits the fluorescence emitted from chlorophyll a, the predominant light harvesting pigment inherent to all (micro)algae, cyanobacteria, and aquatic vascular plants. Measurements of chlorophyll a fluorescence were introduced in the 1960s to improve estimates of phytoplankton abundance. By the 1990s, this field had evolved to enable the physiology and productivity of primary producers to be examined at scales from the single cell to entire ocean basins. Current remote sensing algorithms concentrate on better describing the relationship between primary productivity and natural fluorescence that is emitted from solar excitation. While several major challenges exist in achieving this goal, such approaches provide great promise for accurately characterizing the role of marine primary productivity during this period of extreme climate change. © 2009 Copyright © 2009 Elsevier Ltd. All rights reserved.
© 2009 Elsevier Ltd. Light-absorbing pigments cause many marine organisms to naturally fluoresce, a phenomenon whereby light is emitted at a longer wavelength following light absorption at a shorter wavelength. Fluorescence provides a relatively unobtrusive signature of organism abundance and physiology; consequently, a wide variety of fluorescence techniques are now routinely employed in marine biological investigations. Important technological advances throughout the last 20 years have enabled exploration of the taxonomy and physiology of marine organisms with increasing complexity. In particular, the intracellular use of fluorescent tags, molecules, and dyes that covalently bind to sensing biomolecules. However, many such techniques require samples to be removed from nature. Most widespread for assaying biology in situ exploits the fluorescence emitted from chlorophyll a, the predominant light harvesting pigment inherent to all (micro)algae, cyanobacteria, and aquatic vascular plants. Measurements of chlorophyll a fluorescence were introduced in the 1960s to improve estimates of phytoplankton abundance. By the 1990s, this field had evolved to enable the physiology and productivity of primary producers to be examined at scales from the single cell to entire ocean basins. Current remote sensing algorithms concentrate on better describing the relationship between primary productivity and natural fluorescence that is emitted from solar excitation. While several major challenges exist in achieving this goal, such approaches provide great promise for accurately characterizing the role of marine primary productivity during this period of extreme climate change.
Frommlet, JC, Sousa, L, Alves, A, Vieira, SI, Suggett, DJ & Serodio, J 2015, 'SYMBIODINIUM - THE FIRST DINOFLAGELLATE KNOWN TO DRIVE MICROBIAL-ALGAL CALCIFICATION', EUROPEAN JOURNAL OF PHYCOLOGY, TAYLOR & FRANCIS LTD, pp. 56-57.
Osman, E, Pettay, T, Suggett, DJ, RVoolstra, C, Warner, ME & Smith, D 2015, 'PHYSIOLOGICAL RESPONSE OF SYMBIODINIUM POPULATIONS ADAPTED TO DIFFERENT THERMAL REGIMES IN THE RED SEA', EUROPEAN JOURNAL OF PHYCOLOGY, TAYLOR & FRANCIS LTD, pp. 60-60.
Oxborough, K, Moore, M, Suggett, DJ & Geider, R 2015, 'USING FAST REPETITION RATE FLUOROMETRY TO ESTIMATE PSII ELECTRON FLUX PER UNIT VOLUME: A PURELY OPTICAL METHOD FOR ESTIMATING GPP BY PHYTOPLANKTON?', EUROPEAN JOURNAL OF PHYCOLOGY, TAYLOR & FRANCIS LTD, pp. 34-34.
Warner, ME & Suggett, DJ 2015, 'ESTABLISHING A FUNCTIONAL BASIS TO UNRAVEL SYMBIODINIUM DIVERSITY', EUROPEAN JOURNAL OF PHYCOLOGY, TAYLOR & FRANCIS LTD, pp. 152-153.
Bretherton, L, Lawson, T, Moore, CM, Poulton, A, Geider, R & Suggett, D 2013, 'LIGHT AVAILABILITY AFFECTS THE OCEAN ACIDIFCATION RESPONSE IN COCCOLITHOPHORES', PHYCOLOGIA, INT PHYCOLOGICAL SOC, pp. 13-13.
Lawrenz, E, Huete-Ortega, M, Suggett, DJ & Geider, RJ 2013, 'ASSESSING THE RELATIONSHIPS BETWEEN ELECTRON TRANSFER AND CARBON FIXATION IN NITROGEN LIMITED MARINE PHYTOPLANKTON', PHYCOLOGIA, INT PHYCOLOGICAL SOC, pp. 59-59.
Suggett, DJ 2011, 'PHYSIOLOGICAL SIGNATURES OF MICROALGAL PHOTO-ACCLIMATION AND -ADAPTATION TO ENVIRONMENTAL CHANGE', EUROPEAN JOURNAL OF PHYCOLOGY, TAYLOR & FRANCIS LTD, pp. 34-35.
Suggett, DJ, Lawrenz, E, Silsbe, G, Capuzzo, E, Ylostalo, P, Forster, RM, Simis, S, Prasil, O & Kromkamp, JC 2011, 'TOWARDS PREDICTING THE MINIMUM QUANTUM REQUIREMENT FOR CARBON FIXATION IN EUROPEAN SEAS', EUROPEAN JOURNAL OF PHYCOLOGY, TAYLOR & FRANCIS LTD, pp. 54-54.