I am a microbial ecologist studying bio-geochemical processes at the micro scale via electrochemical microsensors and molecular methods.
In 2010 I completed my PhD thesis on aerobic methane and ammonia transformation in the transient and extremely nutrient loaded environment of floating liquid manure crusts.
I am an Associate within the Plant Functional Biology and Climate Change Cluster (C3), thus bringing microsensor technology to UTS in collaboration with C3 member Professor Michael Kühl.
Initially my research will focus on microbially mediated nitrogen transformations in coral polyps with the aim to discover some of the mechanisms behind the suggested efficient nutrient recycling within coral reef ecosystems
- Micro scale analyses of respiratory and photosynthetic processes in the environment
- Processes controlling bio-geochemical cycling
- Interactions between microbes and higher organisms
- Environmental sustainability
- Technical application of microbes
Brodersen, KE, Trevathan-Tackett, SM, Nielsen, DA, Connolly, RM, Lovelock, CE, Atwood, TB & Macreadie, PI 2019, 'Oxygen consumption and sulfate reduction in vegetated coastal habitats: Effects of physical disturbance', Frontiers in Marine Science, vol. 6, no. FEB.View/Download from: UTS OPUS or Publisher's site
© 2019 Brodersen, Trevathan-Tackett, Nielsen, Connolly, Lovelock, Atwood and Macreadie. Vegetated coastal habitats (VCHs), such as mangrove forests, salt marshes and seagrass meadows, have the ability to capture and store carbon in the sediment for millennia, and thus have high potential for mitigating global carbon emissions. Carbon sequestration and storage is inherently linked to the geochemical conditions created by a variety of microbial metabolisms, where physical disturbance of sediments may expose previously anoxic sediment layers to oxygen (O 2 ), which could turn them into carbon sources instead of carbon sinks. Here, we used O 2 , hydrogen sulfide (H 2 S) and pH microsensors to determine how biogeochemical conditions, and thus aerobic and anaerobic metabolic pathways, vary across mangrove, salt marsh and seagrass sediments (case study from the Sydney area, Australia). We measured the biogeochemical conditions in the top 2.5 cm of surface (0-10 cm depth) and experimentally exposed deep sediments (> 50 cm depth) to simulate undisturbed and physically exposed sediments, respectively, and how these conditions may affect carbon cycling processes. Mangrove surface sediment exhibited the highest rates of O 2 consumption and sulfate (SO 42- ) reduction based on detailed microsensor measurements, with a diffusive O 2 uptake rate of 102 mmol O 2 m -2 d -1 and estimated sulfate reduction rate of 57 mmol S tot2- m -2 d -1 . Surface sediments (0-10 cm) across all the VCHs generally had higher O 2 consumption and estimated sulfate reduction rates than deeper layers (> 50 cm depth). O 2 penetration was < 4 mm for most sediments and only down to 1 mm depth in mangrove surface sediments, which correlated with a significantly higher percent organic carbon content (%C org ) within sediments originating from mangrove forests as compared to those from seagrass and salt marsh ecosystems. Additionally, pH dropped from 8.2 at the sediment/water interface to < 7-7.5 within th...
Petrou, K, Baker, KG, Nielsen, DA, Hancock, AM, Schulz, KG & Davidson, AT 2019, 'Acidification diminishes diatom silica production in the Southern Ocean', Nature Climate Change, vol. 9, no. 10, pp. 781-786.View/Download from: UTS OPUS or Publisher's site
© 2019, The Author(s), under exclusive licence to Springer Nature Limited. Diatoms, large bloom-forming marine microorganisms, build frustules out of silicate, which ballasts the cells and aids their export to the deep ocean. This unique physiology forges an important link between the marine silicon and carbon cycles. However, the effect of ocean acidification on the silicification of diatoms is unclear. Here we show that diatom silicification strongly diminishes with increased acidity in a natural Antarctic community. Analyses of single cells from within the community reveal that the effect of reduced pH on silicification differs among taxa, with several species having significantly reduced silica incorporation at CO2 levels equivalent to those projected for 2100. These findings suggest that, before the end of this century, ocean acidification may influence the carbon and silicon cycle by both altering the composition of the diatom assemblages and reducing cell ballasting, which will probably alter vertical flux of these elements to the deep ocean.
Macreadie, PI, Atwood, TB, Seymour, JR, Fontes, MLS, Sanderman, J, Nielsen, DA & Connolly, RM 2019, 'Vulnerability of seagrass blue carbon to microbial attack following exposure to warming and oxygen.', Science of the Total Environment, vol. 686, pp. 264-275.View/Download from: UTS OPUS or Publisher's site
Seagrass meadows store globally-significant quantities of organic 'blue' carbon. These blue carbon stocks are potentially vulnerable to anthropogenic stressors (e.g. coastal development, climate change). Here, we tested the impact of oxygen exposure and warming (major consequences of human disturbance) on rates of microbial carbon break-down in seagrass sediments. Active microbes occurred throughout seagrass sediment profiles, but deep, ancient sediments (~5000 yrs. old) contained only 3% of the abundance of active microbes as young, surface sediments (<2 yrs. old). Metagenomic analysis revealed that microbial community structure and function changed with depth, with a shift from proteobacteria and high levels of genes involved in sulfur cycling in the near surface samples, to a higher proportion of firmicutes and euraracheota and genes involved in methanogenesis at depth. Ancient carbon consisted almost entirely (97%) of carbon considered 'thermally recalcitrant', and therefore presumably inaccessible to microbial attack. Experimental warming had little impact on carbon; however, exposure of ancient sediments to oxygen increased microbial abundance, carbon uptake and sediment carbon turnover (34-38 fold). Overall, this study provides detailed characterization of seagrass blue carbon (chemical stability, age, associated microbes) and suggests that environmental disturbances that expose coastal sediments to oxygen (e.g. dredging) have the capacity to diminish seagrass sediment carbon stocks by facilitating microbial remineralisation.
Ocean warming is resulting in increased occurrence of mass coral bleaching; a response in which the intracellular algal endosymbionts (Symbiodinium sp.) are expelled from the coral host due to physiological stress. This detrimental process is often attributed to overproduction of reactive oxygen species (ROS) that leak out of the endosymbionts and causes damage to the host cell, though direct evidence validating this link is limited. Here, for the first time, we used confocal microscopy and fluorescent dyes to investigate if endosymbiont ROS production significantly and predictably affects physiological parameters in its host cell. Heat treatment resulted in a 60% reduction in coral symbiont density, a ~70% increase in median endosymbiont ROS and a small reduction in photosystem efficiency (FV/FM, 11%), indicating absence of severe light stress. Notably, no other physiological parameters were affected in either endosymbionts or host cells, including reduced glutathione and ROS-induced lipid peroxidation. Taken together, the increase in endosymbiont ROS could not be linked to physiological damage in either partner, suggesting that oxidative stress is unlikely to have been the driver for symbiont expulsion in this study.
Petrou, K & Nielsen, DA 2018, 'Uptake of dimethylsulphoniopropionate (DMSP) by the diatom Thalassiosira weissflogii: a model to investigate the cellular function of DMSP', BIOGEOCHEMISTRY, vol. 141, no. 2, pp. 265-271.View/Download from: Publisher's site
Petrou, K, Nielsen, DA & Heraud, P 2018, 'Single-cell biomolecular analysis of coral algal symbionts reveals opposing metabolic responses to heat stress and expulsion', Frontiers in Marine Science, vol. 5, no. MAR.View/Download from: UTS OPUS or Publisher's site
© 2018 Petrou, Nielsen and Heraud. The success of corals in nutrient poor environments is largely attributed to the symbiosis between the cnidarian host and its intracellular alga. Warm water anomalies have been shown to destabilize this symbiosis, yet detailed analysis of the effect of temperature and expulsion on cell-specific carbon and nutrient allocation in the symbiont is limited. Here, we exposed colonies of the hard coral Acropora millepora to heat stress and using synchrotron-based infrared microspectroscopy measured the biomolecular profiles of individual in hospite and expelled symbiont cells at an acute state of bleaching. Our results showed symbiont metabolic profiles to be remarkably distinct with heat stress and expulsion, where the two effectors elicited opposing metabolic adjustments independent of treatment or cell type. Elevated temperature resulted in biomolecular changes reflecting cellular stress, with relative increases in free amino acids and phosphorylation of molecules and a concomitant decline in protein content, suggesting protein modification and degradation. This contrasted with the metabolic profiles of expelled symbionts, which showed relative decreases in free amino acids and phosphorylated molecules, but increases in proteins and lipids, suggesting expulsion lessens the overall effect of heat stress on the metabolic signature of the algal symbionts. Interestingly, the combined effects of expulsion and thermal stress were additive, reducing the overall shifts in all biomolecules, with the notable exception of the significant accumulation of lipids and saturated fatty acids. This first use of a single-cell metabolomics approach on the coral symbiosis provides novel insight into coral bleaching and emphasizes the importance of a single-cell approach to demark the cell-to-cell variability in the physiology of coral cellular populations.
Brodersen, KE, Siboni, N, Nielsen, DA, Pernice, M, Ralph, PJ, Seymour, J & Kühl, M 2018, 'Seagrass rhizosphere microenvironment alters plant-associated microbial community composition.', Environmental microbiology, vol. 20, no. 8, pp. 2854-2864.View/Download from: UTS OPUS or Publisher's site
The seagrass rhizosphere harbors dynamic microenvironments, where plant-driven gradients of O2 and dissolved organic carbon form microhabitats that select for distinct microbial communities. To examine how seagrass-mediated alterations of rhizosphere geochemistry affect microbial communities at the microscale level, we applied 16S rRNA amplicon sequencing of artificial sediments surrounding the meristematic tissues of the seagrass Zostera muelleri together with microsensor measurements of the chemical conditions at the basal leaf meristem (BLM). Radial O2 loss (ROL) from the BLM led to ∼ 300 µm thick oxic microzones, wherein pronounced decreases in H2 S and pH occurred. Significantly higher relative abundances of sulphate-reducing bacteria were observed around the meristematic tissues compared to the bulk sediment, especially around the root apical meristems (RAM; ∼ 57% of sequences). Within oxic microniches, elevated abundances of sulphide-oxidizing bacteria were observed compared to the bulk sediment and around the RAM. However, sulphide oxidisers within the oxic microzone did not enhance sediment detoxification, as rates of H2 S re-oxidation here were similar to those observed in a pre-sterilized root/rhizome environment. Our results provide novel insights into how chemical and microbiological processes in the seagrass rhizosphere modulate plant-microbe interactions potentially affecting seagrass health.
Macreadie, PI, Nielsen, DA, Kelleway, JJ, Atwood, TB, Seymour, JR, Petrou, K, Connolly, RM, Thomson, ACG, Trevathan-Tackett, SM & Ralph, PJ 2017, 'Can we manage coastal ecosystems to sequester more blue carbon?', Frontiers in Ecology and the Environment, vol. 15, no. 4, pp. 206-213.View/Download from: UTS OPUS or Publisher's site
© The Ecological Society of America To promote the sequestration of blue carbon, resource managers rely on best-management practices that have historically included protecting and restoring vegetated coastal habitats (seagrasses, tidal marshes, and mangroves), but are now beginning to incorporate catchment-level approaches. Drawing upon knowledge from a broad range of environmental variables that influence blue carbon sequestration, including warming, carbon dioxide levels, water depth, nutrients, runoff, bioturbation, physical disturbances, and tidal exchange, we discuss three potential management strategies that hold promise for optimizing coastal blue carbon sequestration: (1) reducing anthropogenic nutrient inputs, (2) reinstating top-down control of bioturbator populations, and (3) restoring hydrology. By means of case studies, we explore how these three strategies can minimize blue carbon losses and maximize gains. A key research priority is to more accurately quantify the impacts of these strategies on atmospheric greenhouse-gas emissions in different settings at landscape scales.
Trevathan-Tackett, SM, Seymour, JR, Nielsen, DA, Macreadie, PI, Jeffries, TC, Sanderman, J, Baldock, J, Howes, JM, Steven, ADL & Ralph, PJ 2017, 'Sediment anoxia limits microbial-driven seagrass carbon remineralization under warming conditions.', FEMS Microbiology Ecology, vol. 93, no. 6.View/Download from: UTS OPUS or Publisher's site
Seagrass ecosystems are significant carbon sinks, and their resident microbial communities ultimately determine the quantity and quality of carbon sequestered. However, environmental perturbations have been predicted to affect microbial-driven seagrass decomposition and subsequent carbon sequestration. Utilizing techniques including 16S-rDNA sequencing, solid-state NMR and microsensor profiling, we tested the hypothesis that elevated seawater temperatures and eutrophication enhance the microbial decomposition of seagrass leaf detritus and rhizome/root tissues. Nutrient additions had a negligible effect on seagrass decomposition, indicating an absence of nutrient limitation. Elevated temperatures caused a 19% higher biomass loss for aerobically decaying leaf detritus, coinciding with changes in bacterial community structure and enhanced lignocellulose degradation. Although, community shifts and lignocellulose degradation were also observed for rhizome/root decomposition, anaerobic decay was unaffected by temperature. These observations suggest that oxygen availability constrains the stimulatory effects of temperature increases on bacterial carbon remineralization, possibly through differential temperature effects on bacterial functional groups, including putative aerobic heterotrophs (e.g. Erythrobacteraceae, Hyphomicrobiaceae) and sulfate reducers (e.g. Desulfobacteraceae). Consequently, under elevated seawater temperatures, carbon accumulation rates may diminish due to higher remineralization rates at the sediment surface. Nonetheless, the anoxic conditions ubiquitous to seagrass sediments can provide a degree of carbon protection under warming seawater temperatures.
Gardner, SG, Raina, J-B, Nitschke, MR, Nielsen, DA, Stat, M, Motti, CA, Ralph, PJ & Petrou, K 2017, 'A multi-trait systems approach reveals a response cascade to bleaching in corals', BMC BIOLOGY, vol. 15.View/Download from: UTS OPUS or Publisher's site
Petrou, K, Ralph, PJ & Nielsen, DA 2017, 'A novel mechanism for host-mediated photoprotection in endosymbiotic foraminifera.', ISME Journal, vol. 11, no. 2, pp. 453-462.View/Download from: Publisher's site
Light underpins the health and function of coral reef ecosystems, where symbiotic partnerships with photosynthetic algae constitute the life support system of the reef. Decades of research have given us detailed knowledge of the photoprotective capacity of phototrophic organisms, yet little is known about the role of the host in providing photoprotection in symbiotic systems. Here we show that the intracellular symbionts within the large photosymbiotic foraminifera Marginopora vertebralis exhibit phototactic behaviour, and that the phototactic movement of the symbionts is accomplished by the host, through rapid actin-mediated relocation of the symbionts deeper into the cavities within the calcium carbonate test. Using a photosynthetic inhibitor, we identified that the infochemical signalling for host regulation is photosynthetically derived, highlighting the presence of an intimate communication between the symbiont and the host. Our results emphasise the central importance of the host in photosymbiotic photoprotection via a new mechanism in foraminifera that can serve as a platform for exploring host-symbiont communication in other photosymbiotic organisms.
Gardner, SG, Nielsen, DA, Laczka, O, Shimmon, R, Beltran, VH, Ralph, PJ & Petrou, K 2016, 'Dimethylsulfoniopropionate, superoxide dismutase and glutathione as stress response indicators in three corals under short-term hyposalinity stress', PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, vol. 283, no. 1824.View/Download from: UTS OPUS or Publisher's site
Brodersen, KE, Nielsen, DA, Ralph, PJ & Kuhl, M 2015, 'Oxic microshield and local pH enhancement protects Zostera muelleri from sediment derived hydrogen sulphide', NEW PHYTOLOGIST, vol. 205, no. 3, pp. 1264-1276.View/Download from: UTS OPUS or Publisher's site
Gardner, SG, Nielsen, DA, Petrou, K, Larkum, AWD & Ralph, PJ 2015, 'Characterisation of coral explants: a model organism for cnidarian-dinoflagellate studies', CORAL REEFS, vol. 34, no. 1, pp. 133-142.View/Download from: UTS OPUS or Publisher's site
Nielsen, DA, Pernice, M, Schliep, M, Sablok, G, Jeffries, TC, Kuehl, M, Wangpraseurt, D, Ralph, PJ & Larkum, AWD 2015, 'Microenvironment and phylogenetic diversity of Prochloron inhabiting the surface of crustose didemnid ascidians', ENVIRONMENTAL MICROBIOLOGY, vol. 17, no. 10, pp. 4121-4132.View/Download from: UTS OPUS or Publisher's site
Brodersen, KE, Nielsen, DA, Ralph, PJ & Kuhl, M 2014, 'A split flow chamber with artificial sediment to examine the below-ground microenvironment of aquatic macrophytes', MARINE BIOLOGY, vol. 161, no. 12, pp. 2921-2930.View/Download from: UTS OPUS or Publisher's site
Wangpraseurt, D, Polerecky, L, Larkum, A, Ralph, PJ, Nielsen, DA, Pernice, M & Kuhl, M 2014, 'The in situ light microenvironment of corals', Limnology and Oceanography, vol. 59, no. 3, pp. 917-926.View/Download from: UTS OPUS or Publisher's site
We used a novel diver-operated microsensor system to collect in situ spectrally resolved light fields on corals with a micrometer spatial resolution. The light microenvironment differed between polyp and coenosarc tissues with scalar irradiance (400700 nm) over polyp tissue, attenuating between 5.1- and 7.8-fold from top to base of small hemispherical coral colonies, whereas attenuation was at most 1.5-fold for coenosarc tissue. Fluctuations in ambient solar irradiance induced changes in light and oxygen microenvironments, which were more pronounced and faster in coenosarc compared with polyp tissue. Backscattered light from the surrounding benthos contributed . 20% of total scalar irradiance at the coral tissue surface and enhanced symbiont photosynthesis and the local O2 concentration, indicating an important role of benthos optics for coral ecophysiology. Light fields on corals are species and tissue specific and exhibit pronounced variation on scales from micrometers to decimeters. Consequently, the distribution, genetic diversity, and physiology of coral symbionts must be coupled with the measurements of their actual light microenvironment to achieve a more comprehensive understanding of coral ecophysiology.
Nielsen, DA, Schramm, A, Nielsen, LP & Revsbech, NP 2013, 'Seasonal methane oxidation potential in manure crusts', Applied and Environmental Microbiology, vol. 79, no. 1, pp. 407-410.View/Download from: UTS OPUS or Publisher's site
Organic crusts on liquid manure storage tanks harbor ammonia- and nitrite-resistant methane oxidizers and may significantly reduce methane emissions. Methane oxidation potential (0.6 mol CH4 m(-2) day(-1)) peaked during fall and winter, after 4 months of crust development. Consequences for methane mitigation potential of crusts are discussed.
Jokic, T, Borisov, SM, Saf, R, Nielsen, DA, Kuhl, M & Klimant, I 2012, 'Highly photostable near-infrared fluorescent pH indicators and sensors based on BF2-chelated tetraarylazadipyrromethene dyes', Analytical Chemistry, vol. 84, pp. 6723-6730.View/Download from: UTS OPUS or Publisher's site
In this study, a series of new BF2-chelated tetraarylazadipyrromethane dyes are synthesized and are shown to be suitable for the preparation of on/off photoinduced electron transfer modulated fluorescent sensors. The new indicators are noncovalently entrapped in polyurethane hydrogel D4 and feature absorption maxima in the range 660â 710 nm and fluorescence emission maxima at 680â740 nm. Indicators have high molar absorption coefficients of â¼80 000 Mâ1 cmâ1, good quantum yields (up to 20%), excellent photostability and low cross-sensitivity to the ionic strength. pKa values of indicators are determined from absorbance and fluorescence measurements and range from 7 to 11, depending on the substitution pattern of electron-donating and -withdrawing functionalities. Therefore, the new indicators are suitable for exploitation and adaptation in a diverse range of analytical applications. Apparent pKa values in sensor films derived from fluorescence data show 0.5â1 pH units lower values in comparison with those derived from the absorption data due to FoÌrster resonance energy transfer from protonated to deprotonated form. A dual-lifetime referenced sensor is prepared, and application for monitoring of pH in corals is demonstrated.
Kofoed, MV, Nielsen, DA, Revsbech, NP & Schramm, A 2012, 'Fluorescence in situ hybridization (FISH) detection of nitrite reductase transcripts (nirS mRNA) in Pseudomonas stutzeri biofilms relative to a microscale oxygen gradient', Systematic and Applied Microbiology, vol. 35, no. 8, pp. 513-517.View/Download from: UTS OPUS or Publisher's site
Microsensor measurements of oxygen were combined with mRNA-targeted ?uorescence in situ hybridization (FISH) to relate the expression of nitrite reductase (nirS) to oxygen concentrations in arti?- cial bio?lms of the denitri?er Pseudomonas stutzeri. A distinct zone of nirS transcript-containing cells was detected at the oxicanoxic transition zone, below an oxygen threshold concentration of 0.72.5 M, depending on incubation conditions. Although not a routine technique yet, the possibility of coupling microsensor and mRNA-targeted FISH analyses described here opens for studies addressing microenvironment, identity, and actual activity of microbes in strati?ed environments at single cell resolution
Nielsen, DA, Nielsen, LP, Schramm, A & Revsbech, NP 2010, 'Oxygen Distribution and Potential Ammonia Oxidation in Floating, Liquid Manure Crusts', Journal of Environmental Quality, vol. 39, no. 5, pp. 1813-1820.View/Download from: UTS OPUS or Publisher's site
Floating, organic crusts on liquid manure, stored as a result of animal production, reduce emission of ammonia (NH(3)) and other volatile compounds during storage. The occurrence of NO(2)(-) and NO(3)(-) in the crusts indicate the presence of actively metabolizing NH(3)-oxidizing bacteria (AOB) which may be partly responsible for this mitigation effect. Six manure tanks with organic covers (straw and natural) were surveyed to investigate the prevalence and potential activity of AOB and its dependence on the O(2) availability in the crust matrix as studied by electrochemical profiling. Oxygen penetration varied from <1 mm in young, poorly developed natural crusts and old straw crusts, to several centimeters in the old natural crusts. The AOB were ubiquitously present in all crusts investigated, but nitrifying activity could only be detected in old natural crusts and young straw crust with high O(2) availability. In old natural crusts, total potential NH(3) oxidation rates were similar to reported fluxes of NH(3) from slurry without surface crust. These results indicate that old, natural surface crusts may develop into a porous matrix with high O(2) availability that harbors an active population of aerobic microorganisms, including AOB. The microbial activity may thus contribute to a considerable reduction of ammonia emissions from slurry tanks with well-developed crusts.
Hansen, RR, Nielsen, DA, Schramm, A, Nielsen, LP, Revsbech, NP & Hansen, M 2009, 'Greenhouse Gas Microbiology in Wet and Dry Straw Crust Covering Pig Slurry', Journal of Environmental Quality, vol. 38, no. 3, pp. 1311-1319.View/Download from: UTS OPUS or Publisher's site
Liquid manure (Slurry) storages are sources of gases Such ammonia (NH(3)) and methane (CH(4)). Danish slurry storages are required to be covered to reduce NH(3) emissions and often a floating crust of straw is applied. This study investigated whether physical properties of the crust or crust microbiology had an effect oil the emission of the potent greenhouse gases CH(4) and nitrous oxide (N(2)O) when crust moisture was manipulated ("dry", "moderate", and "wet"). The dry crust had the deepest oxygen penetration (45 mm as compared to 20 mm in the wet treatment) as measured with microsensors, the highest amounts of nitrogen oxides (NO(2)(-) and NO(3)(-)) (up to 36 mu mol g(-1) wet weight) and the highest emissions of N(2)O and CH(4). Fluorescent in situ hybridization and gene-specific polymerase chain reaction (PCR) were used to detect occurrence of bacterial groups. Ammonia-oxidizing bacteria (AOB) were abundant in all three crust types, whereas nitrite-oxidizing bacteria (NOB) were undetectable and methane-oxidizing bacteria (MOB) were only sparsely present in the wet treatment. A change to anoxia did not affect the CH(4) emission indicating the virtual absence of aerobic methane oxidation in the investigated 2-mo old crusts. However, all increase in N(2)O emission was observed in all crusted treatments exposed to anoxia, and this was probably a result of denitrification based oil NO(x)(-) chat had accumulated in the crust during oxic conditions. To reduce overall greenhouse gas emissions, floating crust should be managed to optimize conditions for methanotrophs.
Ottosen, L, Poulsen, H, Nielsen, DA, Finster, K, Nielsen, LP & Revsbech, NP 2009, 'Observations on microbial activity in acidified pig slurry', Biosystems Engineering, vol. 102, no. 3, pp. 291-297.View/Download from: UTS OPUS or Publisher's site
Acidification of pig slurry to pH 5.5 is used as a measure to reduce ammonia emission from pits and storages. The slurry is acidified with sulphuric acid in a process tank and pumped back to the slurry pits or to a storage tank. We investigated the effect of acidification on microbial activity. Oxygen consumption rate, methanogenesis and sulphate reduction were all reduced by more than 98% in the stored acidified slurry compared to untreated slurry. Despite higher sulphate concentration, the microbial metabolism was greatly compromised or absent in the acidified slurry. This could be explained by the high concentration of protonized short-chained volatile fatty acids in the acidified slurry (approximately 25 mM, compared to untreated slurry <0.1 mM), which act as an uncoupling agent of the cell membrane potential and thereby arrest microbial metabolism. In total the consequences of slurry acidification are greatly reduced production rates and loss of sulphide and methane, and eliminated loss of ammonia. On the other hand, increased volatilization and loss of smelly fatty acids is to be expected.
Brodersen, KE, Kühl, M, Nielsen, DA, Pedersen, O & Larkum, AWD 2018, 'Rhizome, root/sediment interactions, aerenchyma and internal pressure changes in seagrasses' in Seagrasses of Australia: Structure, Ecology and Conservation, Springer, Germany, pp. 393-418.View/Download from: UTS OPUS or Publisher's site
© Springer International Publishing AG, part of Springer Nature 2018. Life in seawater presents several challenges for seagrasses owing to low O 2 and CO 2 solubility and slow gas diffusion rates. Seagrasses have evolved numerous adaptations to these environmental conditions including porous tissue providing low-resistance internal gas channels (aerenchyma) and carbon concentration mechanisms involving the enzyme carbonic anhydrase. Moreover, seagrasses grow in reduced, anoxic sediments, and aerobic metabolism in roots and rhizomes therefore has to be sustained via rapid O 2 transport through the aerenchyma. Tissue aeration is driven by internal concentration gradients between leaves and belowground tissues, where the leaves are the source of O 2 and the rhizomes and roots function as O 2 sinks. Inadequate internal aeration e.g., due to low O 2 availability in the surrounding water during night time, can lead to sulphide intrusion into roots and rhizomes, which has been linked to enhanced seagrass mortality. Under favourable conditions, however, seagrasses leak O 2 and dissolved organic carbon into the rhizosphere, where it maintains oxic microzones protecting the plant against reduced phytotoxic compounds and generates dynamic chemical microgradients that modulate the rhizosphere microenvironment. Local radial O 2 loss from belowground tissues of seagrasses leads to sulphide oxidation in the rhizosphere, which generates protons and results in local acidification. Such low-pH microniches can lead to dissolution of carbonates and protolytic phosphorus solubilisation in carbonate-rich sediments. The seagrass rhizosphere is also characterised by numerous high-pH microniches indicative of local stimulation of proton consuming microbial processes such as sulphate reduction via root/rhizome exudates and/or release of alkaline substances. High sediment pH shifts the sulphide speciation away from H 2 S towards non-tissue-penetrating HS - ions, which can alleviate the be...
Seymour, JR, Laverock, B, Nielsen, DA, Trevathan-Tackett, SM & Macreadie, PI 2018, 'The microbiology of seagrasses' in Seagrasses of Australia: Structure, Ecology and Conservation, pp. 343-392.View/Download from: Publisher's site
© Springer International Publishing AG, part of Springer Nature 2018. Like both terrestrial plants and other benthic marine organisms, seagrasses host abundant and diverse communities of microorganisms. These microbes fundamentally influence seagrass physiology and health, while also regulating the biogeochemical dynamics of entire seagrass meadows. Discrete populations of bacteria, fungi, microalgae, archaea and viruses inhabit seagrass leaves, roots and rhizomes and the surrounding sediments. The plethora of ecological interactions taking place between seagrasses and this microbiome span the continuum of symbiotic relationships from mutualism to parasitism. Indeed, the metabolic activities of some seagrass associated microbes, such as diazotrophic and sulphur oxidizing bacteria, govern the local chemical environment in ways that facilitate seagrass survival. On the other hand, pathogens, such as the protozoan parasite Labyrinthula cause disease outbreaks that can lead to mass seagrass die offs. While the role of the seagrass microbiome in defining the success of seagrass habitats is becoming increasingly apparent, there is still much to be learnt. For instance, the development of an understanding of how seagrass associated microbes may buffer or augment the negative impacts of growing environmental pressures will be valuable for informing decisions regarding the management and conservation of threatened seagrass habitats. In this chapter we will synthesise the current state of knowledge on the microbiology of seagrasses, with a goal of conveying the often overlooked importance of the seagrass microbiome in governing seagrass health and the biogeochemical stability of seagrass ecosystems.