Barratt, J, Kaufer, A, Peters, B, Craig, D, Lawrence, A, Roberts, T, Lee, R, McAuliffe, G, Stark, D & Ellis, J 2017, 'Isolation of Novel Trypanosomatid, Zelonia australiensis sp. nov. (Kinetoplastida: Trypanosomatidae) Provides Support for a Gondwanan Origin of Dixenous Parasitism in the Leishmaniinae.', PLoS Neglected Tropical Diseases, vol. 11, no. 1, pp. 1-26.View/Download from: Publisher's site
The genus Leishmania includes approximately 53 species, 20 of which cause human leishmaniais; a significant albeit neglected tropical disease. Leishmaniasis has afflicted humans for millennia, but how ancient is Leishmania and where did it arise? These questions have been hotly debated for decades and several theories have been proposed. One theory suggests Leishmania originated in the Palearctic, and dispersed to the New World via the Bering land bridge. Others propose that Leishmania evolved in the Neotropics. The Multiple Origins theory suggests that separation of certain Old World and New World species occurred due to the opening of the Atlantic Ocean. Some suggest that the ancestor of the dixenous genera Leishmania, Endotrypanum and Porcisia evolved on Gondwana between 90 and 140 million years ago. In the present study a detailed molecular and morphological characterisation was performed on a novel Australian trypanosomatid following its isolation in Australia's tropics from the native black fly, Simulium (Morops) dycei Colbo, 1976. Phylogenetic analyses were conducted and confirmed this parasite as a sibling to Zelonia costaricensis, a close relative of Leishmania previously isolated from a reduviid bug in Costa Rica. Consequently, this parasite was assigned the name Zelonia australiensis sp. nov. Assuming Z. costaricensis and Z. australiensis diverged when Australia and South America became completely separated, their divergence occurred between 36 and 41 million years ago at least. Using this vicariance event as a calibration point for a phylogenetic time tree, the common ancestor of the dixenous genera Leishmania, Endotrypanum and Porcisia appeared in Gondwana approximately 91 million years ago. Ultimately, this study contributes to our understanding of trypanosomatid diversity, and of Leishmania origins by providing support for a Gondwanan origin of dixenous parasitism in the Leishmaniinae.
Lawrence, AL, Brown, GK, Peters, B, Spielman, DS, Morin-Adeline, V & Šlapeta, J 2014, 'High phylogenetic diversity of the cat flea (Ctenocephalides felis) at two mitochondrial DNA markers.', Medical and veterinary entomology, vol. 28, no. 3, pp. 330-336.View/Download from: Publisher's site
The cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae) (Bouché), is the most common flea species found on cats and dogs worldwide. We investigated the genetic identity of the cosmopolitan subspecies C. felis felis and evaluated diversity of cat fleas from Australia, Fiji, Thailand and Seychelles using mtDNA sequences from cytochrome c oxidase subunit I (cox1) and II (cox2) genes. Both cox1 and cox2 confirmed the high phylogenetic diversity and paraphyletic origin of C. felis felis. The African subspecies C. felis strongylus (Jordan) is nested within the paraphyletic C. felis felis. The south East Asian subspecies C. felis orientis (Jordan) is monophyletic and is supported by morphology. We confirm that Australian cat fleas belong to C. felis felis and show that in Australia they form two distinct phylogenetic clades, one common with fleas from Fiji. Using a barcoding approach, we recognize two putative species within C. felis (C. felis and C. orientis). Nucleotide diversity was higher in cox1 but COX2 outperformed COX1 in amino acid diversity. COX2 amino acid sequences resolve all phylogenetic clades and provide an additional phylogenetic signal. Both cox1 and cox2 resolved identical phylogeny and are suitable for population structure studies of Ctenocephalides species.
Webb, G, Miller, PF & Peters, BA 2012, 'Pyriproxyfen For The Control Of Australian Salt-marsh Mosquito, Aedes Vigilax', Journal of the American Mosquito Control Association, vol. 28, no. 1, pp. 50-52.View/Download from: Publisher's site
The efficacy of pyriproxyfen against the Australian salt-marsh mosquito, Aedes vigilax, was examined in 2 laboratory and 1 semi-field study using both technical grade and formulated products. In a dose-response study, the median emergence inhibition (EI5
Greive, K, Staton, J, Miller, PF, Peters, BA & Oppenheim, V 2010, 'Development Of Melaleuca Oils As Effective Natural-Based Personal Insect Repellents', Australian Journal of Entomology, vol. 49, no. 1, pp. 40-48.View/Download from: Publisher's site
For many decades effective insect repellents have relied on synthetic actives such as N,N-diethyl-metatoluamide. Increasingly, consumers are seeking natural-based alternatives to many everyday products including insect repellents. While many studies have
An increase in the number of anecdotal reports of bedbug infestations i short-stay lodge type accommodation used by backpackers and other budget travellers inthe City of Sydney promoted a pilot survery to determine the extent of the problem. The aim of the survey was to obatin the perspective of the lodge managers on bedbug problems and how they handled them. This article describes the survey results and assesses the effectiveness of the methods of control reported.
Miller, PF, Peters, BA & Hort, CA 2001, 'A field study to evaluate integrated flea control using Lufenuron and Nitenpyram compared to Imidacloprid used alone', Australian Veterinary Practitioner, vol. 31, no. 2, pp. 60-66.
A one-year field study was carried out in 45 households in Cairns, North Queensland to evaluate the efficacy of three topical or systemic treatment programs in controlling cat flea (Ctenocephalides felis) infestations within the residence and on dogs and cats. Homes were randomly assigned to one of three treatments. In the first group of homes, lufenuron was administered orally to all household pets according to label recommendations for the study duration, and nitenpyram was administered orally once per week for the first 6 weeks. After 6 weeks, all pets in the household received a single dose of nitenpyram if fleas were observed on any animal. Treatments in the second group of homes were identical to those for the first group except that all pets in the household received two doses of nitenpyram per week. In the final group, imidacloprid was applied topically to all household pets once every 4 weeks for the duration of the study, and no other flea-control measures were applied. Flea populations on the pets of all treatment groups were assessed; environmental flea numbers were assessed only for Groups 1 and 3. The combination of lufenuron and nitenpyram provided superior control of flea populations on the animals and in the environment compared with using imidacloprid alone.
Miller, PF, Peters, BA & Vernon, F 2005, 'A field study on the control of European wasp Vespula germanica (Hymenoptera: Vespidae) and paper wasp Polisted sp. (Hymenoptera: Vespidae) using a novel aqueous powder formulation', Proceedings of the Fifth International Conference on Urban Pests, Perniagaan Ph'ng @P&Y Design Network, Malaysia, pp. 387-391.
Peters, BA, Miller, PF & Staton, JA 2005, 'A study on a novel personal insect repellent formulation against the mosquito (Diptera: Culicidae), the biting midge (Diptera: Ceratopogonidae) and the bush fly (Diptera: Miscidae)', Proceedings of the Fifth International Conference on Urban Pests, Singapore.
Miller, PF & Peters, BA 2002, 'Comparative field study of a new formulation of Goliath Cockroach Gel against German Cockroach (Dictyoptera: Blattellidae) and a Mixed Population of American Cockroach and Australian Cockroach (Dictyoptera: Blattidae)', Proceedings of the 4th International Conference on Urban Pests, South Carolina.
Miller, PF, Peters, BA & Scrivener, AM 2002, 'The efficacy of eight commercial cockroach baits against the American cockroach (Dictyoptera: Blattidae)', Proceedings of the 4th International Conference on Urban Pests, South Carolina.
Peters, BA, Miller, PF & Scrivener, AM 2002, 'The Efficacy of Eight Commercial Cockroach Baits against the American Cockroach (Dictyoptera: Blattidae)', Proceedings of the 4th International Conference on Urban Pests, South Carolina, USA, pp. 446-446.
Miller, PF & Peters, BA 1999, 'Performance of Goliath cockroach gel against German cockroach (Blattodea: Blattellidae) and a mixed population of American cockroach and Australian cockroach (Blattodea: Blattidae) in the field', Proceedings of the Third International Conference on Urban Pests, Graficke zavody Hronov, Prague.
Miller, PF & Peters, BA 1999, 'The efficacy of Imidacloprid gel at various rates against German cockroach (Blattodea: Blattellidea)', Proceedings of the 3rd International Conference on Urban Pests, Prague.