Anna is a plant stress physiologist, specializing in toxicology. Her research focuses on the optimization of algal culture growth and community composition for bioremediation, and assessment of toxicity of treated water to species of various trophic levels.
Prior to joining UTS:C3 in 2019, Anna completed a Ph.D. in Ecotoxicology at Masaryk University in Brno (Czech Republic). She conducted her experimental work at the Global Change Research Institute CAS (Czech Republic) at the Department of Adaptive Biotechnologies and during long-term research stays at the University of Copenhagen (Denmark) and Estación Experimental del Zaidín CSIC (Spain). Her research focused on (i) establishment of a multi-sensor system based on fluorescence imaging for high-throughput screening of early toxic stress in plants and plant cell cultures, and (ii) improvements in cultivation of photoautotrophic plant cell cultures.
- Tissue cultures
- Fluorescence-based imaging techniques
- Plant and algal stress physiology
- Photoautotrophic plant cell cultures
- Principal component analysis
Segečová, A, Pérez-Bueno, ML, Barón, M, Červený, J & Roitsch, TG 2019, 'Noninvasive determination of toxic stress biomarkers by high-throughput screening of photoautotrophic cell suspension cultures with multicolor fluorescence imaging.', Plant methods, vol. 15, no. 1.View/Download from: UTS OPUS or Publisher's site
Background:With increasing pollution, herbicide application and interest in plant phenotyping, sensors capturing early responses to toxic stress are demanded for screening susceptible or resistant plant varieties. Standard toxicity tests on plants are laborious, demanding in terms of space and material, and the measurement of growth-inhibition based endpoints takes relatively long time. The aim of this work was to explore the potential of photoautotrophic cell suspension cultures for high-throughput early toxicity screening based on imaging techniques. The investigation of the universal potential of fluorescence imaging methods involved testing of three toxicants with different modes of action (DCMU, glyphosate and chromium). Results:The increased pace of testing was achieved by using non-destructive imaging methods-multicolor fluorescence (MCF) and chlorophyll fluorescence (ChlF). These methods detected the negative effects of the toxicants earlier than it was reflected in plant growth inhibition (decrease in leaf area and final dry weight). Moreover, more subtle and transient effects not resulting in growth inhibition could be detected by fluorescence. The pace and sensitivity of stress detection was further enhanced by using photoautotrophic cell suspension cultures. These reacted sooner, more pronouncedly and to lower concentrations of the tested toxicants than the plants. Toxicant-specific stress signatures were observed as a combination of MCF and ChlF parameters and timing of the response. Principal component analysis was found to be useful for reduction of the collected multidimensional data sets to a few informative parameters allowing comparison of the toxicant signatures. Conclusions:Photoautotrophic cell suspension cultures have proved to be useful for rapid high-throughput screening of toxic stress and display a potential for employment as an alternative to tests on whole plants. The MCF and ChlF methods are capable of distinguishing early stress sig...
Segecova, A, Cerveny, J & Roitsch, T 2018, 'Advancement of the cultivation and upscaling of photoautotrophic suspension cultures using Chenopodium rubrum as a case study', PLANT CELL TISSUE AND ORGAN CULTURE, vol. 135, no. 1, pp. 37-51.View/Download from: UTS OPUS or Publisher's site
Červený, J, Zavřel, T, Chmelík, D, Búzová, D, Fedorko, J, Literáková, P, Sukačová, K, Segecova, A & Roitsch, T 2016, 'Microalgal adaptive biotechnologies for a sustainable future' in Vačkář, D & Janouš, D (eds), Global Change & Ecosystems, Global Change Research Institute, Czech Academy of Sciences, pp. 148-160.